Abstract
We have extended the experimental system of Mugnier and Tepfer (1985) to study root-pathogen interactions in vitro. This experimental system effectively simulates the interactions of roots and pathogens when roots are attached to whole plants and growing in the soil. The system consists of plant roots genetically transformed by Agrobacterium rhizogenes and a bicompartmental root culture system (BRC system). Using the BRC system we have obtained infections, in vitro, of Plasmodiophora brassicae on transformed rape and kohlrabi roots and of Polymyxa betae on transformed sugar beet roots. The study of the biology of certain host-pathogen interactions and agrichemical screening and research in crop disease resistance have been enhanced by the BRC system.
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© 1986 Martinus Nijhoff Publishers, Dordrecht
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Mugnier, J., Ready, P.W., Riedel, G.E. (1986). Root culture system useful in the study of biotrophic root pathogens in vitro. In: Augustine, P.C., Danforth, H.D., Bakst, M.R. (eds) Biotechnology for Solving Agricultural Problems. Beltsville Symposia in Agricultural Research, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-4396-4_11
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DOI: https://doi.org/10.1007/978-94-009-4396-4_11
Publisher Name: Springer, Dordrecht
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