Abstract
Yeasts have held great promise as hosts for production of economically important proteins and peptides using recombinant DNA technology. Among the heralded features of the bakers yeast, Saccharomyces cerevisiae, are its safety (lack of toxicity, pyrogenicity and allergenicity), ability to be grown on an industrial scale, and the vast wealth of information regarding its genetics and molecular biology which has led to the development of a number of expression vector systems. Invariably these vectors are based on the endogenous multicipy plasmid, 2 micron circle, and use a variety of promoter systems, either constitutive or regulated, to drive the expression of the desired gene [1] [2].
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References
DeWilde M, Cabezon T, Harford N, Rutgers T, Simoen E, and Wijnerdaele F van. (1983) “Eighteenth Congress Joint IABDS/WHO Symposium on Standardization and Control of Biologicals Produced by Recombinant DNA Technology, Geneva Switzerland, Vol 59 Pp. 99–107.
Bitter GA and Egan KM. (1984) Gene 32, 263–274.
Hitzeman RA, Chen CY, Hagie FE, Patzer EJ, Lio C, Estell DA, Miller JV, Yaffe A, Kleid DG, Levinson AD and Opperman H. (1983) Nucleic Acids Research 11, 2745–2763.
Hailing SM and Smith S. (1985) Biotechnology 13, 715–726.
Ballou CE. (1982) “The Molecular Biology of the Yeast Saccharomyces” (Strathern J, Jones E, Broach J). Cold Spring Harbor Laboratory, Vol. II Pp. 335–360.
Williams RS, Trumbly RJ, MacGoll R, Trimble RB and Maley F. (1985) The Journal of Biological Chemistry 260, 13334–13341.
Brunt J van. (1986) Biotechnology 4, 505–510.
Taitkamradt AG, Turner KJ, Kramer RA, Elliott QD, Bostian SJ, Thill GP, Rogers DT and Bostian KA. (1986) Molecular and Cellular Biology 6, 1855–1865.
Murray AW and Szostak JW. (1983) Cell 34, 961–970.
Srienc F, Campbell JL and Bailey JE. (1986) Biotechnology and Bioengineering 28, 996–1006.
Parulekar SJ, Semones GB, Rolf MJ, Lievense JC and Lim HC. (1986) Biotechnology & Bioengineering 28, 700–710.
Brunt J van. (1986) Biotechnology 4, 395–401.
Ellis S, Brust P, Waters A, Koutz PJ, Harpold M and Gingeras TR. (1985) Molecular and Cellular Biology 5, 1111–1121.
Francis DP. (1983) Infectious Diseases Review 5, 322–335.
Peterson DL, Roberts IM and Vyas GN. (1977) Proceedings of the National Academy of Sciences USA 74, 1530–1534.
Tiollais P, Charnay P, Vyas GN. (1981) Science 213, 406–411.
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© 1987 Martinus Nijhoff Publishers, Dordrecht
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Thill, G. et al. (1987). The Methylotrophic Yeast Pichia Pastoris as a Host for Heterologous Protein Production. In: van Verseveld, H.W., Duine, J.A. (eds) Microbial Growth on C1 Compounds. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3539-6_35
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DOI: https://doi.org/10.1007/978-94-009-3539-6_35
Publisher Name: Springer, Dordrecht
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