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Fixation of Kidney Tissue for Morphometric Study

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Nephrotoxicity in the experimental and clinical situation

Part of the book series: Developments in Nephrology ((DINE,volume 19-20))

Abstract

The classical biochemical approach to studying an organ has been to homogenize it. This technique has provided much of the present body of knowledge on intermediary metabolism, the nature of metabolites and the essential basic processes carried out in all cells. This “grind and measure” approach is especially successful in tissues and cell populations which are homogeneous. The more heterogeneous the collection of cells, the more the procedure based on destroying cell biological organization is seen to be limited in its usefulness. The kidney is heterogeneous at both a gross and a microscopic level. Cortex and medulla, though playing parts in the same overall process of urine production, are biochemically very dissimilar. Within the cortex and medulla there are numerous types of cells, each with special properties, many of which are highly directional. As a consequence the cells are polarized both along the kidney tubule length, and across its width on an axis from tubule lumen to tubule basement membrane. Methodologies that permit the study of chemical processes as they occur spatially, i.e. “anatomically based” approaches, are thus particularly pertinent to the study of the kidney and of nephrotoxicity.

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Authors
  1. M. A. Williams
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  2. J. I. Lowrie
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Editor information

Editors and Affiliations

  1. The Robens Institute, University of Surrey, Guildford, Surrey, England

    P. H. Bach

  2. Biochemical Toxicology Section, Imperial Chemical Industries plc, Alderley Park, Macclesfield, Cheshire, England

    E. A. Lock

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© 1987 Martinus Nijhoff Publishers

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Williams, M.A., Lowrie, J.I. (1987). Fixation of Kidney Tissue for Morphometric Study. In: Bach, P.H., Lock, E.A. (eds) Nephrotoxicity in the experimental and clinical situation. Developments in Nephrology, vol 19-20. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3367-5_4

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  • DOI: https://doi.org/10.1007/978-94-009-3367-5_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-8012-5

  • Online ISBN: 978-94-009-3367-5

  • eBook Packages: Springer Book Archive

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