Abstract
European Aspen(Populus tremula), quaking aspen (P. tremuloi-des) and their hybrids are fast-growing, short rotation trees that have a wide range of adaptability and can be grown on marginal sites. Aspen wood has been employed in matchstick, paper-and plywood industries and has potential value for biomass and energy production. Vegetative propagation of aspen is extremely difficult by woody cuttings and uneconomical by root suckers and grafting. Consequently, large scale clonal multiplication of superior trees has not been feasible with conventional methods of vegetative propagation. On the other hand, tissue culture technology has been successfully exploited for large scale clonal propagation of aspen clones.
The present study was undertaken to determine the plant regeneration capacity and its association with growth performance, the genotype-culture media interaction and the optimal dose of 6-benzylaminopurine (BA) for tissues obtained from 15 clones of five aspen and hybrid aspen families.
It was found that there exists an optimal concentration of BA. The variation of microshoots per explant was significantly large for BA-treatments, families and clones within families. Certain families showed considerable genotype-culture media interaction. The regeneration capacity seems to be independent of the growth performance under field conditions, which is advantageous for breeding purposes.
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© 1988 Kluwer Academic Publishers
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Ahuja, M.R., Krusche, D., Melchior, G.H. (1988). Determination of Plantlet Regeneration Capacity of Selected Aspen Clones in Vitro . In: Ahuja, M.R. (eds) Somatic Cell Genetics of Woody Plants. Forestry Sciences, vol 30. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2811-4_15
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DOI: https://doi.org/10.1007/978-94-009-2811-4_15
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