Abstract
Recently, the spectrum of secondary metabolites which might be produced in good levels by tissue culture techniques, has been extended by the use of organ cultures, especially of transformed root cultures. These hairy root cultures accumulate usually the spectrum of secondary metabolites in levels as found in roots of the intact plant. Nevertheless for technological purposes growth and product levels of many root cultures have to be optimized. Thus we wanted to analyze whether secondary pathways of root cultures would be affected by the same factors as known from studies with normal cultures. The ease of manipulating secondary pathways of suspension cultures has provided first insight into their regulatory controls. As activities of enzymes diverting primary metabolites into secondary pathways are often greatly induced under culture conditions favoring secondary metabolism, it has been suggested that these enzymes serve as one of diverse regulatory controls of secondary pathways. Therefore we were interested to see whether such enzymes undergo rapid changes in transformed root or suspension cultures. The availability of cell lines with different productivities let us compare the activities of such enzymes. Here we summarize some of our experiences in altering isoflavonoid levels of Lupinus and ß-carbolines and serotonin of Peganum by variation of the medium composition.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Berlin, J., C. Mollenschott, F. Sasse, L. Witte, H.G. Piehl, H. Büntemeyer (1987) J. Plant Physiol. 131, 225–236.
Berlin, J., C. Mollenschott, F. Sasse, L. Witte, H.G. Piehl, H. Büntemeyer (1987) J. Plant Physiol. 131, 225–236.
Sasse, F., J. Hammer, J. Berlin (1980) J. Chromatogr. 194, 234–238.
Berlin, J., K.-H. Knobloch, G. Höfle, L. Witte (1982) J.Nat.Prod. 45, 83–87.
Berlin, J., M. Rippert, C. Mollenschott, F. Maywald, D. Strack, V. Wray, C. Sator (1989) Planta Medica 55, 685–686.
Berlin, J. (1988) In: Production of metabolites by plant cell cultures. APRIA, Paris p. 89–98.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Kluwer Academic Publishers
About this chapter
Cite this chapter
Berlin, J., Mollenschott, C., Greidziak, N., Erdogan, S., Kuzovkina, I. (1990). Affecting Secondary Product Formation in Suspension and Hairy Root Cultures — a Comparison. In: Nijkamp, H.J.J., Van Der Plas, L.H.W., Van Aartrijk, J. (eds) Progress in Plant Cellular and Molecular Biology. Current Plant Science and Biotechnology in Agriculture, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2103-0_114
Download citation
DOI: https://doi.org/10.1007/978-94-009-2103-0_114
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7445-2
Online ISBN: 978-94-009-2103-0
eBook Packages: Springer Book Archive