Summary
The Liquid-impelled Loop Reactor (LLR), a new type of density-difference mixed bioreactor, can be an interesting tool for the application of biocatalysis in two-phase systems. The LLR combines the advantages of the use of an ALR with those of two-phase systems.
These advantages will be more evident when the biocatalysts used are not inhibited by the solvent used in this type of fermenter and when the product formed will preferentially be transfered into the organic phase.
The cells used in this study are Rubia tinctorum and Morinda citrifolia, that produce anthraquinones, rather hydrophobic compounds.
First, organic solvents having no harmful effect on growth or production as compared to a one-phase system were selected. Then growth and production kinetics of these cells in the presence of the selected organic solvents were characterized.
The results of these experiments were used to start the LLR with hexadecane as the second phase and to compare the results with the performance of an ALR.
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Buitelaar, R.M., Susaeta, I., Tramper, J. (1990). Application of the Liquid-Impelled Loop Reactor for the Production of Anthraquinones by Plant Cell Cultures. In: Nijkamp, H.J.J., Van Der Plas, L.H.W., Van Aartrijk, J. (eds) Progress in Plant Cellular and Molecular Biology. Current Plant Science and Biotechnology in Agriculture, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2103-0_104
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