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Polytene chromosome preparation from anopheline mosquitoes

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The Molecular Biology of Insect Disease Vectors

Abstract

Within certain Dipteran tissues chromatids replicate repeatedly without the occurrence of any mitosis-like events. This phenomenon — known as endoreplication — leads to the production of thousands of sister chromatids within the same nucleus resulting in more extended axial parts and a number of more contracted chromomeric loops of different size. The outcome are the so-called polytene (or giant) chromosomes, which appear as bundles of extended interphase chromatids characterised by peculiar banding patterns and by an axial length approximately 100–200 times the length of the same chromosomes during mitotic metaphase. Such chromosomes can easily be observed under a compound microscope (Figure 28.1).

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References

  • Bryan, J.H. and Coluzzi, M. (1971) Cytogenetic observations on Anopheles favrauti Laveran. Bull. WHO45, 266–267.

    PubMed  CAS  Google Scholar 

  • Bryan, J.H., Petrarca V., Di Deco, M.A. and Coluzzi, M. (1987) Adult behaviour of members of the Anopheles gambiae complex in the Gambia with special reference to An. melas and its chromosomal variants. Parassitologia29, 221–249.

    PubMed  CAS  Google Scholar 

  • Coluzzi, M. (1966) Osservazioni comparative sul cromosoma X nelle specie A e B del comm-lesso Anopheles gambiae. Rc. Accad. Naz.. Lincei40, 671–678.

    Google Scholar 

  • Coluzzi, M. (1968) Cromosomi politenici delle cellule nutrici ovariche del complesso gambiae del genere Anopheles. Parassitologia10, 179–184.

    Google Scholar 

  • Coluzzi, M. (1982) Spatial distribution of chromosomal inversions and speciation in anophe-line mosquitoes. In Mechanisms of Speciation (C Barigozzi, ed.), pp. 143–149. A.R. Liss Inc., New York.

    Google Scholar 

  • Coluzzi, M., Petrarca V. and Di Deco, M.A. (1985) Chromosomal inversion intergradation and incipient speciation in Anopheles gambiae. Boll. Zool.52, 45–63.

    Article  Google Scholar 

  • Coluzzi, M., Sabatini, A., Petrarca, V. and Di Deco, M.A. (1979) Chromosomal differentiation and adaptation to human environments in the Anopheles gambiae complex. Trans. R. Soc. Trop. Med. Hyg.73, 483–497.

    Article  PubMed  CAS  Google Scholar 

  • Conger, A.D. and Fairchild, L.M. (1953) A quick-freeze method for making smear slides permanent. Stain Tech.28, 281–283.

    CAS  Google Scholar 

  • Davidson, G. and Hunt, R. (1973) The crossing and chromosome characteristics of a new sixth species in the Anopheles gambiae complex. Parassitologia15, 121–128.

    PubMed  CAS  Google Scholar 

  • French, W.L., Baker, R.H. and Kitzmiller, J.B. (1962) Preparation of mosquito chromosomes. Mosquito News, 22, 377–383.

    Google Scholar 

  • Frizzi, G. (1947) Salivary gland chromosomes of Anopheles. Nature160, 226–227.

    Article  Google Scholar 

  • Frizzi, G. (1953) Etude cytogénétique d’Anopheles maculipennis en Italic Bull. Wld. Hlth. Org.9, 335–344.

    CAS  Google Scholar 

  • Green, C.A. (1982) Cladistic analysis of mosquito chromosome data of Anopheles (Celia) Myzomyia. J. Hered.73, 2–11.

    PubMed  CAS  Google Scholar 

  • Green, CA. and Miles, S.J. (1980) Chromosomal evidence for sibling species of the malaria vector Anopheles (Celia) culicifacies Giles. J. Trop. Med. Hyg.83, 75–78.

    PubMed  CAS  Google Scholar 

  • Green, CA., Baimai, V., Harrison, B.A. and Andre, R.G. (1985) Cytogenetic evidence for a complex of species within the taxon Anopheles maculatus (Diptera: Culicidae). Biology J. Linnean Soc.24, 321–336.

    Article  Google Scholar 

  • Hunt, R.H. (1973) A cytological technique for the study of Anopheles gambiae complex. Parassitologia, XV, 137–139.

    Google Scholar 

  • Kitzmiller, J.B., Frizzi, G. and Baker, R.H. (1967) Evolution and speciation within the maculipennis complex of the genus insect vectors of disease. In Genetics of insect vectors of disease (J.W. Wright and R. Pal, eds), pp.151–158. Elsevier, Amsterdam.

    Google Scholar 

  • Mahon, R.J. (1983) Identification of the three sibling species of Anopheles farauti Laveran by the banding pattern of their polytene chromosomes. J. Aust. Entomol. Soc.22, 31–34.

    Article  Google Scholar 

  • Mahon, R.J. and Miethke, P.M. (1982) Anopheles farauti No.3, a hitherto unrecognized biological species of mosquito within the taxon A. farauti Laveran (Diptera: Culicide). Trans. R. Soc. Trop. Med. Hyg.76, 8–12.

    Article  PubMed  CAS  Google Scholar 

  • Petrarca, V. and Beier, J.C. (1992) Intraspedfk chromosomal polymorphism in the Anopheles gambiae complex as a factor affecting malaria transmission in the Kisumu area of Kenya. Am. J. Trop. Med. Hyg.46, 229–237.

    PubMed  CAS  Google Scholar 

  • Sorsa, V. (1988) Polytene chromosomes in genetic research. Ellis Horwood Ltd, England.

    Google Scholar 

  • Subbarao, S.K. Vasantha, K., Adak, T. and Sharma, V.P. (1983) Anopheles culicifacies complex: Evidence for a new sibling species, species C. Ann. Entomol. Soc. Am.76, 985–988.

    Google Scholar 

  • White, G.B. (1973) Comparative studies on sibling species of the Anopheles gambiae Giles complex (Dipt. Culicidae): III. The distribution, ecology, behaviour and vectorial importance of species D in Bwambae County, Uganda, with an analysis of biological, ecological, morphological and cytogenetical ralationships of Ugandan species D. Bull. Entomol. Res.63, 65–97.

    Article  Google Scholar 

  • White, G.B., Coluzzi, M. and Zahar, A.R. (1975) Review of cytogenetic studies on anopheline vectors of malaria. WHO Techn. Rep. Mal/75.849, VBC/75.538. Annex 2, 14–20.

    Google Scholar 

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della Torre, A. (1997). Polytene chromosome preparation from anopheline mosquitoes . In: Crampton, J.M., Beard, C.B., Louis, C. (eds) The Molecular Biology of Insect Disease Vectors. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1535-0_28

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  • DOI: https://doi.org/10.1007/978-94-009-1535-0_28

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7185-7

  • Online ISBN: 978-94-009-1535-0

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