Abstract
The complement-dependent bactericidal activities of rabbit polyclonal and mouse monoclonal anti-OMP-MC antibodies were evaluated against homologous and heterologous strains of serum-resistant gonococci. Rabbit anti-OMP-MC promoted killing of homologous FA 19 organisms and two of three heterologous strains at dilutions up to 1:200; mouse IgM monoclonal promoted killing of homologous organisms. Bactericidal activities were not influenced by the presence of proteins II. Partial proteolysis of OMP-MC indicated that antibodies in bactericidal sera bound to papain-sensitive portions of the complex that represent <25% of the mass of OMP-MC.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Hansen, M. V. & C. E. Wilde, III (1984) Conservation of peptide structure of OMP-MC from Neisseria gonorrhoeae. Infect. Immun. 43: 839–845
Newhall, W. J., C. E. Wilde, III, W. D. Sawyer & R. A. Haak (1980) High molecular weight antigenic protein complex in the outer membrane of Neisseria gonorrhoeae. Infect. Immun. 27: 475–482
Schoolnik, G. K., T. M. Buchanan & K. K. Holmes (1976) Gonococci causing disseminated gonococcal infection are resistant to the bactericidal action of normal human sera. J. Clin. Invest. 58: 1163–1173
Virji, M. & J. E. Heckels (1985) Role of anti-pilus antibodies in host defense against gonococcal infection studied with monoclonal anti-pilus antibodies. Infect. Immun. 49: 621–628
Virji, M., K. Zak & J. E. Heckels (1986) Monoclonal antibodies to gonococcal outer membrane protein IB: Use in investigation of the protective effect of antibodies directed against conserved and type-specific epitopes. J. Gen. Micro. 132: 1621–1629
Wilde, C. E. III & M. V. Hansen (1985) Serological characterization of Outer Membrane Protein- Macromolecular Complex from Neisseria gonorrhoeae and other members of the family Neisseria-ceae. In: G. K. Schoolnik (Ed) The Pathogenic Neisseriae (pp. 37 - 45 ). ASM Publications. Washington, D.C.
Zollinger, W. D. & R. E. Mandrell (1983) Importance of complement source in bactericidal activity of human antibody and murine monoclonal antibody to meningococcal group B polysaccharide. Infect. Immun. 40: 257–264
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Kluwer Academic Publishers, Dordrecht
About this chapter
Cite this chapter
Corbett, M.J., Black, J.R., Wilde, C.E. (1988). Antibodies to outer membrane protein — macromolecular complex (OMP-MC) are bactericidal for serum-resistant gonococci. In: Poolman, J.T. (eds) Gonococci and Meningococci. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1383-7_107
Download citation
DOI: https://doi.org/10.1007/978-94-009-1383-7_107
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7117-8
Online ISBN: 978-94-009-1383-7
eBook Packages: Springer Book Archive