Abstract
Nitrogen fixing Enterobacter agglomerans strains, isolated from the innermost rhizosphere of wheat, contained all genes essential for nitrogen fixation on large (100–150 kb) indigenous plasmids. One of these plasmids, pEA9, was marked with either Tn1725 or Tn5, and studied with regard to its transmissibility by conjugation with other recipient strains. In a homologous system (cured parental strains as recipients) the rate of transfer was in the order of 10-5 per recipient cell. It could be drastically increased by introduction of a mob site (on Tn5) and offering plasmid RP4 as helper. However, the host range was narrow. To obtain nif-plasmids with a wide host range, the nif-genes have been cut out and ligated to a broad host range vector, pRK415, derivative of RP4. These studies are a prerequisite for later releasing experiments.
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© 1989 Kluwer Academic Publishers
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Klingmüller, W., Herterich, S., Min, BW. (1989). Self-transmissible nif-plasmids in Entevobacter . In: Skinner, F.A., Boddey, R.M., Fendrik, I. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 35. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0889-5_21
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DOI: https://doi.org/10.1007/978-94-009-0889-5_21
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