Skip to main content

High Speed Analytical and Preparative Separations of Biological Macromolecules

  • Chapter
Separations for Biotechnology 2

Abstract

A wide pore, 4000A, macroporous resin has been developed which is rigid and able to operate under HPLC conditions of flow rate and pressure. The poly(styrene/divinylbenzene) matrix can be used for reversed-phase separations and for both anion and cation exchange chromatography after surface coating/derivatization.

The open pore structure of this resin makes it suitable for the HPLC analysis of biological macromolecules due to the significant improvement in mass transfer. The rigidity and rapid equilibration enables small columns, 50 x 4.6mm, to be operated with steep gradients at high flow rates for both high resolution analytical separations and high load preparative fractionations.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Guiochan G., Martin M., J. Chromatogr., 326 (1985) 3

    Article  Google Scholar 

  2. Unger K.K., Janzen R., Jilge G., Chromatographia;, 24 (1987) 144

    Article  CAS  Google Scholar 

  3. Unger K.K., Jilge G., Kinkel J.N., Hearn M.T.W., J. Chromatogr., 397 (1987) 91

    Article  PubMed  Google Scholar 

  4. Anspach B., Unger K.K., Giesche H., Hearn M.T.W., 11th International Symposium on HPLC of Proteins, Peptides and Polynucleotides, Baltimore, December 1984.

    Google Scholar 

  5. Burke D.J., Duncan J.K., Siebart C., Ott G.S., J. Chromatogr., 359 (1986) 533

    Article  PubMed  CAS  Google Scholar 

  6. Lloyd L.L., Warner F.P., J. Chromatogr; in press

    Google Scholar 

  7. Dawkins J.V., Lloyd L.L., Warner F.P., J. Chromatogr., 352 (1986) 157

    Article  CAS  Google Scholar 

  8. Bristow P.A., LC in Practise, hetp, Cheshire, 1976, p. 41.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1990 SCI

About this chapter

Cite this chapter

Lloyd, L.L., Warner, F.P. (1990). High Speed Analytical and Preparative Separations of Biological Macromolecules. In: Pyle, D.L. (eds) Separations for Biotechnology 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0783-6_42

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-0783-6_42

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6839-0

  • Online ISBN: 978-94-009-0783-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics