Abstract
Reaction centers (RCs) from purple photosynthetic bacteria have been used as a model system for the study of herbicides which act as competitive inhibitors of the secondary quinone, QB, of the acceptor quinone complex (1). The x-ray structures of RCs for Rps. viridis (2) and Rb. sphaeroides (3) have revealed some details of the QB/herbicide binding domain, as well as features of the interaction. Many herbicide resistant mutants have been isolated by various groups by selecting cells that grow in the presence of herbicide (4,5). However, it is now possible to learn more about the structure/function relationships of the RC by selectively altering any amino acid in the QB binding domain through site-directed mutagenesis. Here, we report the construction of a RC deletion mutant of Rb. sphaeroides which can be complemented by mutant RC genes to produce mutant RC proteins. Preliminary characterization of a site-directed mutant at Tyr L222 in the QB binding domain of the RC is also presented.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Wraight, CA. (1981) Israel J. Chem. 21,348–354.
Michel, H., Epp, O., and Deisenhofer, J. (1986) EMBO J. 5,2445–2451.
Allen, J.P., Feher, G., Yeates, T.O., Komiya, H., and Reese, D.C. (1987) Proc. Natl. Acad. Sci. USA 84,6162–6166.
Stein, R.R., Castellvi, A.L., Bogacz, J.P., and Wraight, CA. (1984) J. Cell. Biochem. 24,243–259.
Paddock, M.L., Rongey, S.H., Abresch, E.C., Feher, G. and Okamura, M.Y. (1988) Photosynthesis Res. 17,75–96.
Williams, J.C., Steiner, LA. and Feher, G. (1986) Proteins 1,312–325.
Simon, R, Preifer, U. and Puhler, A. (1983) Biotechnology 1:784–791.
Davis, J., Donohue, T.J. and Kaplan, S. (1988) J. Bacteriol. 170,320–329.
Mead, DA, Szczena-Scorupa, E., and Kemper, B. (1986) Protein Engineering 1:67–74.
Kunkle, TA. (1987) in Current protocols in molecular biology (Ausubel, F.M. et al. eds.) pp. 8.8.1.–8.8.6. John Wiley and Sons, New York.
Ditta, G., Schmidhauser, T., Yakobson, E., Lu, P., Iiang, X-W., Finlay, D.R., Guiney, D. and Helinski, D.R. (1985) 13,149–153.
Jolchine, G. and Reiss-Husson, F. (1974) FEBS Lett. 40,5–8.
Allen, J.P., Feher, G., Yeates, T.O., Komiya, H., and Reese, D.C. (1988) Proc. Natl. Acad. Sci. USA 85,8487–8491.
Paddock, M.L., Rongey, S.H., Feher, G., and Okamura, M.Y. (1989) Proc. Acad. Sci. USA, in press.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer Science+Business Media New York
About this chapter
Cite this chapter
Takahashi, E., Maroti, P., Wraight, C.A. (1990). Site-Directed Mutagenesis of Rhodobacter sphaeroides Reaction Center: The Role of Tyrosine L222. In: Baltscheffsky, M. (eds) Current Research in Photosynthesis. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0511-5_36
Download citation
DOI: https://doi.org/10.1007/978-94-009-0511-5_36
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6716-4
Online ISBN: 978-94-009-0511-5
eBook Packages: Springer Book Archive