Abstract
Restriction Fragment Length Polymorphism (RFLP) analysis is based on two techniques that are widely used in modern molecular biology: the restriction endonuclease digestion of DNA and the transfer of DNA fragments to a filter, onto which can then be hybridized a labelled DNA fragment (1). Type II restriction endonucleases of bacteria recognize and cut specific nucleotide motifs in a DNA sequence (the enzymes commonly used for RFLP analysis recognize 4–6 base-pair sequences). They are, therefore, capable of reducing complex DNA, such as plant DNA, to a population of fragments with discrete sizes. In practice, fragments range in size from a few to more than several thousand base-pairs.
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Brettschneider, R. (1998). RFLP Analysis. In: Karp, A., Isaac, P.G., Ingram, D.S. (eds) Molecular Tools for Screening Biodiversity. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0019-6_18
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DOI: https://doi.org/10.1007/978-94-009-0019-6_18
Publisher Name: Springer, Dordrecht
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