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Advances in Sequencing the Barley Genome

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Genomics of Plant Genetic Resources

Abstract

Barley genome sequencing is lagging behind the status achieved for many other crop genomes although barley is ranking worldwide as fifth most important crop species. Whole genome sequencing of barley with classical Sanger sequencing technology was long meant to be too costly due to the very large genome size of more than 5 Gigabases. By the introduction of Next Generation Sequencing technology this situation has changed and fascinating new possibilities opened up for in depth barley genome analysis and whole genome sequencing. Genome composition has been revealed at unprecedented resolution. A linear gene order map comprising two thirds of all barley genes could be developed and the approach is currently adopted for other related and important cereal genomes like wheat and rye. Important technical limitations have been solved making even whole genome sequencing in barley a feasible endeavor. Provided these new possibilities, it is becoming obvious that soon sequencing per se is no longer the limiting factor but sequence assembly remains the challenge. This review will provide a brief summary of the recent developments in barley genome sequencing achieved since the introduction of Next Generation Sequencing.

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Authors and Affiliations

  1. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466, Gatersleben, Germany

    Nils Stein & Burkhard Steuernagel

Authors
  1. Nils Stein
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  2. Burkhard Steuernagel
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Correspondence to Nils Stein .

Editor information

Editors and Affiliations

  1. Agricultural Sciences, University of Bologna, Bologna, Italy

    Roberto Tuberosa

  2. Genebank, IPK, Gatersleben, Sachsen-Anhalt, Germany

    Andreas Graner

  3. Bioversity International, Rome, Italy

    Emile Frison

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Stein, N., Steuernagel, B. (2014). Advances in Sequencing the Barley Genome. In: Tuberosa, R., Graner, A., Frison, E. (eds) Genomics of Plant Genetic Resources. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7572-5_16

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