Abstract
Arbuscular mycorrhizal (AM) symbiosis can protect the host plants against the detrimental effects of the water deficit caused by osmotic stresses such as drought and salinity. Stomatal conductance (gs) and water use efficiency (WUE) are among the most studied water relations parameters in the mycorrhizal literature, since they are considered critical to the long-term performance of host plants in semiarid environments. Mycorrhizal effects on gs have been observed in about 50% of experiments involving AM and nonAM plants of similar size. In fact, gs rates usually are higher in AM than in nonAM plants, which implies that AM plants have a lower resistance to vapour transfer from inside the leaves to the atmosphere. AM and nonAM plants have also shown different critical points or thresholds of stomatal behaviour during drought episodes. The higher gs rates in AM plants have been associated with lower xylem-sap abscisic acid (ABA) and lower ABA fluxes to leaves in AM plants. On the other hand, it has been suggested that extraradical hyphae or increased root branching may allow mycorrhizal roots to better explore a particular soil volume, extending soil water depletion zones and giving a mycorrhizal root system more access to available water. In addition, it has been estimated that about half of the promotion of gs by AM fungi can be attributable to the soil colonization by AM fungi. Nevertheless, these results can vary when the host plant shows a water conservative strategy. Moreover, different AM fungal species have been shown to modulate also differently the physiological response, including gs, of host plant to drought. The AM influence on gs can also be modulated by environmental conditions such as irradiance, air temperature or leaf temperature. There are also several reports in the literature showing an increase of plant WUE by the AM symbiosis either under well watered or under osmotic stress conditions. The effects of AM symbiosis on WUE depend on the fungal species involved, without a correlation with the percentage of root infection. These effects have been rather related to higher net photosynthetic rate and optimal quantum yield of photosystem II in AM plants than in nonAM ones and with enhanced activities of carbon assimilatory enzymes such as Rubisco. In any case, specific studies dealing with the effect of AM symbiosis on leaf morphology are needed in order to ascertain how these parameters influence the WUE of the host plant.
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Abbreviations
- ABA:
-
Abscisic acid
- AM:
-
Arbuscular mycorrhiza
- AMF:
-
Arbuscular mycorrhizal fungus
- gs :
-
Stomatal conductance
- LAR:
-
Leaf area ratio
- NAR:
-
Net assimilation rate
- QY:
-
Optimal quantum yield of photosystem II
- SLW:
-
Specific leaf weight
- Ψ:
-
Water potential
- WUE:
-
Water use efficiency
References
Allen MF (1991) The ecology of mycorrhizae. Cambridge University Press, Cambridge
Allen MF, Boosalis MG (1983) Effects of two species of VA mycorrhizal fungi on drought tolerance of winter wheat. New Phytol 93:67–76
Araus JL, Bort J, Steduto P, Villegas D, Royo C (2003) Breeding cereals for Mediterranean conditions: ecophysiology clues for biotechnology application. Ann Appl Biol 142:129–141
Aroca R, Irigoyen JJ, Sánchez-Díaz M (2003) Drought enhances maize chilling tolerance II. Photosynthetic traits and protective mechanisms against oxidative stress. Physiol Plant 117:540–549
Aroca R, Tognoni F, Irigoyen JJ, Sánchez-Díaz M, Pardossi A (2001) Different root low temperature response of two maize genotypes differing in chilling sensitivity. Plant Physiol Biochem 39:1067–1073
Augé RM (2000) Stomatal behavior of arbuscular mycorrhizal plants. In: Kapulnik Y, Douds DD (eds) Arbuscular mycorrhizas: physiology and function. Kluwer, Dordrecht, The Netherlands, pp. 201–237. ISBN 0-7923-6444-9
Augé RM (2001) Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza 11:3–42
Augé RM (2004) Arbuscular mycorrhizae and soil/plant water relations. Can J Soil Sci 84:373–381
Augé RM, Duan X (1991) Mycorrhizal fungi and nonhydraulic root signals of soil drying. Plant Physiol 97:821–824
Augé RM, Duan X, Ebel RC, Stodola AJ (1994) Nonhydraulic signaling of soil drying in mycorrhizal maize. Planta 193:74–82
Augé RM, Foster JG, Loescher WH, Stodola AW (1992a) Symplastic sugar and free amino acid molality of Rosa roots with regard to mycorrhizal colonization and drought. Symbiosis 12:1–17
Augé RM, Moore JL, Sylvia DM, Cho K (2004a) Mycorrhizal promotion of host stomatal conductance in relation to irradiance and temperature. Mycorrhiza 14:85–92
Augé RM, Schekel KA, Wample RL (1986) Osmotic adjustment in leaves of VA mycorrhizal nonmycorrhizal rose plants in response to drought stress. Plant Physiol 82:765–770
Augé RM, Stodola AJ, Brown MS, Bethlenfalvay GJ (1992b) Stomatal response of mycorrhizal cowpea and soybean to short-term osmotic stress. New Phytol 120:117–125
Augé RM, Stodola AJ, Ebel RC, Duan XR (1995) Leaf elongation and water relations of mycorrhizal sorghum in response to partial soil drying: two Glomus species at varying phosphorus fertilization. J Exp Bot 46:297–307
Augé RM, Stodola AW, Tims JE, Saxton AM (2001) Moisture retention properties of a mycorrhizal soil. Plant Soil 230:87–97
Augé RM, Sylvia DM, Park SJ, Buttery BR, Saxton AM, Moore JL, Cho K (2004b) Partitioning mycorrhizal influence on water relations of Phaseolus vulgaris into soil and plant components. Can J Bot 82:503–514
Augé RM, Toler HD, Moore JL, Cho K, Saxton AM (2007) Comparing contributions of soil versus root colonization to variations in stomatal behavior and soil drying in mycorrhizal Sorghum bicolor and Cucurbita pepo. J Plant Physiol 164:1289–1299
Augé RM, Toler HD, Sams CE, Nasim G (2008) Hydraulic conductance and water potential gradients in squash leaves showing mycorrhiza-induced increases in stomatal conductance. Mycorrhiza 18:115–121
Baker NR (2008) Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol 59:89–113
Berta G, Trotta A, Fusconi A, Hooker JE, Munro M, Atkinson D, Giovannetti M, Morini S, Fortuna P, Tisserant B, Gianinazzi-Pearson V, Gianinazzi S (1995) Arbuscular mycorrhizal induced changes to plant growth and root system morphology in Prunus cerasifera. Tree Physiol 15:281–293
Blum A (2005) Drought resistance, water-use efficiency, and yield potential – are they compatible, dissonant, or mutually exclusive? Aust J Agric Res 56:1159–1168
Bray EA (2004) Genes commonly regulated by water-deficit stress in Arabidopsis thaliana. J Exp Bot 55:2331–2341
Bray SR, Kitajima K, Sylvia DM (2003) Mycorrhizae differentially alter growth, physiology, and competitive ability of an invasive shrub. Ecol Appl 13:565–574
Bryla DR, Duniway JM (1998) The influence of the mycorrhiza Glomus etunicatum on drought acclimation in safflower and wheat. Physiol Plant 104:87–96
Caravaca F, Díaz E, Barea JM, Azcón-Aguilar C, Roldán A (2003) Photosynthetic and transpiration rates of Olea europaea subsp. sylvestris and Rhamnus lycioides as affected by water deficit and mycorrhiza. Biol Plant 46:637–639
Cho K, Toler HD, Lee J, Ownley BH, Jean C, Stutz JC, Moore JL, Augé RM (2006) Mycorrhizal symbiosis and response of sorghum plants to combined drought and salinity stresses. J Plant Physiol 163:517–528
Craufurd PQ, Wheeler TR, Ellis RH, Summerfield RJ, Williams JH (1999) Effect of temperature and water deficit on water-use efficiency, carbon isotope discrimination, and specific leaf area in peanut. Crop Sci 39:136–142
Davies WJ, Tardieu F, Trejo CL (1994) How do chemical signals work in plants that grow in drying soil? Plant Physiol 104:309–314
Denby K, Gehring C (2005) Engineering drought and salinity tolerance in plants: lessons from genome-wide expression profiling in Arabidopsis. Trends Biotechnol 23:547–552
Druge U, Schonbeck F (1992) Effect of vesicular–arbuscular mycorrhizal infection on transpiration, photosynthesis and growth of flax (Linum usitatissimum L.) in relation to cytokinin levels. J Plant Physiol 141:40–48
Duan X, Neuman DS, Reiber JM, Green CD, Saxton AM, Augé RM (1996) Mycorrhizal influence on hydraulic and hormonal factors implicated in the control of stomatal conductance during drought. J Exp Bot 47:1541–1550
Ebel RC, Duan X, Still DW, Augé RM (1997) Xylem sap abscisic acid concentration and stomatal conductance of mycorrhizal Vigna unguiculata in drying soil. New Phytol 135:755–761
Ebel RC, Welbaum GE, Gunatilaka M, Nelson T, Augé RM (1996) Arbuscular mycorrhizal symbiosis and nonhydraulic signaling of soil drying in Vigna unguiculata (L.) Walp. Mycorrhiza 6:119–127
Estrada-Luna AA, Davies FT Jr, Egilla JN (2000) Mycorrhizal fungi enhancement of growth and gas exchange of micropropagated guava plantlets (Psidium guajava L.) during ex vitro acclimatization and plant establishment. Mycorrhiza 10:1–8
Franks P (2006) Higher rates of leaf gas exchange are associated with higher leaf hydrodynamic pressure gradients. Plant Cell Environ 29:584–592
Ge Y, Chang J, Li WC, Sheng HY, Yue CL, Shan GYS (2003) Effect of soil moisture on the gas exchange of Changium smyrniodes and Anthriscus sylvestris. Biol Plant 47:605–608
Ghannoum O, von Caemmerer S, Conroy JP (2001) Carbon and water economy of australian NAD-ME and NADP-ME C-4 grasses. Aust J Plant Physiol 28:213–223
Goicoechea N, Antolin MC, Sánchez-Díaz M (1997) Gas exchange is related to the hormone balance in mycorrhizal or nitrogen-fixing alfalfa subjected to drought. Physiol Plant 100:989–997
Goicoechea N, Merino S, Sánchez-Díaz M (2004) Contribution of arbuscular mycorrhizal fungi (AMF) to the adaptations exhibited by the deciduous shrub Anthyllis cytisoides under water deficit. Physiol Plant 122:453–464
Green CD, Stodola A, Augé RM (1998) Transpiration of detached leaves from mycorrhizal and nonmycorrhizal cowpea and rose plants given varying abscisic acid, pH, calcium and phosphorus. Mycorrhiza 8:93–99
Hardie K (1985) The effect of removal of extraradical hyphae on water uptake by vesicular-arbuscular mycorrhizal plants. New Phytol 101:677–684
Kaya C, Higgs D, Kirnak H, Tas I (2003) Mycorrhizal colonisation improves fruit yield and water use efficiency in watermelon (Citrullus lanatus Thunb.) grown under well-watered and water stressed conditions. Plant Soil 253:287–292
Khalvati MA, Hu Y, Mozafar A, Schmidhalter U (2005) Quantification of water uptake by arbuscular mycorrhizal hyphae and its significance for leaf growth, water relations, and gas exchange of barley subjected to drought stress. Plant Biol 7:706–712
Khan IA, Ayub N, Mirza SN, Nizami SN, Azam M (2008) Yield and water use efficiency (WUE) of Cenchrus ciliaris as influenced by vesicular arbuscular mycorrhizae (VAM). Pak J Bot 40:931–937
Klingeman WE, van Iersel MW, Kang JG, Augé RM, Moore JL, Flanagan PC (2005) Whole-plant gas exchange measurements of mycorrhizal ‘Iceberg’ roses exposed to cyclic drought. Crop Protect 24:309–317
Kothari SK, Marschner H, George E (1990) Effect of VA mycorrhizal fungi and rhizosphere microorganisms on root and shoot morphology, growth and water relations in maize. New Phytol 116:303–311
Kramer PJ, Boyer JS (1997) Water relations of plants and soils. Academic, San Diego, CA
Kubikova E, Moore JL, Ownlew BH, Mullen MD, Augé RM (2001) Mycorrhizal impact on osmotic adjustment in Ocimum basilicum during a lethal drying episode. J Plant Physiol 158:1227–1230
Loreto F, Centritto M (2008) Leaf carbon assimilation in a water-limited world. Plant Biosyst 142:154–161
Lovelock CE, Kyllo D, Winter K (1996) Growth responses to vesicular-arbuscular mycorrhizae and elevated CO2 in seedlings of a tropical tree, Beislchmiedia pendula. Funct Ecol 10:662–667
Marulanda A, Azcón R, Ruiz-Lozano JM (2003) Contribution of six arbuscular mycorrhizal fungal isolates to water uptake by Lactuca sativa L. plants under drought stress. Physiol Plant 119:526–533
Masumoto C, Ishii T, Hatanaka T, Uchida N (2005) Mechanisms of high photosynthetic capacity in BC2F4 lines derived from a cross between Oryza sativa and wild relatives O-rufipogon. Plant Prod Sci 8:539–545
Munné-Bosch S, Alegre L (2004) Die and let live: leaf senescence contributes to plant survival under drought stress. Funct Plant Biol 31:203–216
Munné-Bosch S, Nogués S, Alegre L (1998) Daily patterns of photosynthesis of two Mediterranean Schrubs response to water deficit. In: Garab G (ed) Photosynthesis: mechanisms and effects. Kluwer, Dordrecht, pp 4015–4018
Osundina M (1995) Responses of seedlings of Parkia biglobes (African locust bean) to drought and inoculation with vesicular-arbuscular mycorrhiza. Nigerian J Bot 8:1–10
Poorter H, Remkes C (1990) Leaf-area ratio and net assimilation rate of 24 wild-species differing in relative growth-rate. Oecologia 83:553–559
Porcel R, Barea JM, Ruiz-Lozano JM (2003) Antioxidant activities in mycorrhizal soybean plants under drought stress and their possible relationship to the process of nodule senescence. New Phytol 157:135–143
Porcel R, Ruiz-Lozano JM (2004) Arbuscular mycorrhizal influence on leaf water potential, solute accumulation and oxidative stress in soybean plants subjected to drought stress. J Exp Bot 55:1743–1750
Querejeta JI, Allen MF, Alguacil MM, Roldán A (2007) Plant isotopic composition provides insight into mechanisms underlying growth stimulation by AM fungi in a semiarid environment. Funct Plant Biol 34:683–691
Querejeta JI, Allen MF, Caravaca F, Roldan A (2006) Differential modulation of host plant delta 13C and delta 18O by native and nonnative arbuscular mycorrhizal fungi in a semiarid environment. New Phytol 169:379–387
Querejeta JI, Barea JM, Allen MF, Caravaca F, Roldan A (2003) Differential response of delta 13C and water use efficiency to arbuscular mycorrhizal infection in two aridland woody plant species. Oecologia 135:510–515
Rood SB, Braatne JH, Hughes FMR (2003) Ecophysiology of riparian cottonwoods: stream flow dependency, water relations and restoration. Tree Physiol 23:1113–1124
Ruiz-Lozano JM (2003) Arbuscular mycorrhizal symbiosis and alleviation of osmotic stress. New perspectives for molecular studies. Mycorrhiza 13:309–317
Ruiz-Lozano JM, Azcón R (1995) Hyphal contribution to water uptake in mycorrhizal plants as affected by the fungal species and water status. Physiol Plant 95:472–478
Ruiz-Lozano JM, Azcón R, Gómez M (1995a) Effects of arbuscular mycorrhizal Glomus species on drought tolerance: physiological and nutritional plant responses. Appl Environ Microbiol 61:456–460
Ruiz-Lozano JM, Gómez M, Azcón R (1995b) Influence of different Glomus species on the time-course of physiological plant responses of lettuce to progressive drought stress periods. Plant Sci 110:37–44
Ruiz-Lozano JM, Azcón R, Palma JM (1996) Superoxide dismutase activity in arbuscular-mycorrhizal Lactuca sativa L. plants subjected to drought stress. New Phytol 134:327–333
Ruiz-Lozano JM, Collados C, Barea JM, Azcón R (2001) Arbuscular mycorrhizal symbiosis can alleviate drought-induced nodule senescence in soybean plants. New Phytol 151:493–502
Sánchez-Blanco MJ, Ferrández T, Morales MA, Morte A, Alarcón JJ (2004) Variations in water status, gas exchange, and growth in Rosmarinus officinalis plants infected with Glomus deserticola under drought conditions. J Plant Physiol 161:675–682
Scheidegger Y, Saurer M, Bahn M, Siegwolf R (2000) Linking stable oxygen and carbon isotopes with stomatal conductance and photosynthetic capacity: a conceptual model. Oecologia 125:350–357
Seki M, Kamei A, Yamaguchi-Shinozaki K, Shinozaki K (2003) Molecular responses to drought, salinity and frost: common and different paths for plant protection. Cur Op Biotechnol 14:194–199
Sheng M, Tang M, Chen H, Yang B, Zhang F, Huang Y (2008) Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza 18:287–296
Songsri P, Jogloy S, Holbrook CC, Kesmala T, VoraSoot N, Akkasaeng C, Patanothai A (2009) Association of root, specific leaf area, and SPAD chlorophyll meter reading to water use efficiency of peanut under different available soil water. Agric Water Manage 96:790–798
Tambussi EA, Bort J, Araus JL (2007) Water use efficiency in C3 cereals under Mediterranean conditions: a review of physiological aspects. Ann Appl Biol 150:307–321
Valentine AJ, Mortimer PE, Lintnaar A, Borgo R (2006) Drought responses of arbuscular mycorrhizal grapevines. Symbiosis 41:127–133
van der Heijden MGA (2004) Arbuscular mycorrhizal fungi as support systems for seedling establishment in grasslands. Ecol Lett 7:293–303
Varma A (2008) Mycorrhiza. State of the art, genetics and molecular biology, Eco-funcion, biotecnology, eco-physiology, structure and systematics, 3rd edn. Springer, Berlin Heidelberg
Wu QS, Xia RX (2006) Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions. J Plant Physiol 163:417–425
Zhu JK, Hasegawa PM, Bressan R (1997) Molecular aspects of osmotic stress in plants. Crit Rev Plant Sci 16:253–277
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The work was supported by a grant from Ministerio de Ciencia e Inovación, Spain (project AGL2008-00898).
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Ruiz-Lozano, J.M., Aroca, R. (2010). Host Response to Osmotic Stresses: Stomatal Behaviour and Water Use Efficiency of Arbuscular Mycorrhizal Plants. In: Koltai, H., Kapulnik, Y. (eds) Arbuscular Mycorrhizas: Physiology and Function. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9489-6_11
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