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Chlorophyll Fluorescence Terminology: An Introduction

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Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications

Part of the book series: Developments in Applied Phycology ((DAPH,volume 4))

Abstract

The terminology used to describe the various components of variable chlorophyll fluorescence has evolved as our understanding of variable chlorophyll fluorescence has increased. For the newcomer to in vivo chlorophyll a (chl-a) fluorescence studies one of the most confusing aspects can be the large number of terms and notations used, many of which often refer to the same parameter. Several proposals to standardise fluorescence notation, most notably by van Kooten and Snel (1990) and Maxwell and Johnson (2000), have reduced the extent of this variation in more recent publications, but some variation still occurs (Baker and Oxborough 2004). Furthermore, in recent years the notation used necessarily has become more complex as new instruments have allowed researchers to apply several techniques within a single study. On such occasions it is essential that notation also distinguishes between techniques (e.g. single turnover vs. multiple turnover) or method (e.g. steady-state light curve vs. non-steady-state light curve).

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Notes

  1. 1.

    DCMU must be added in total darkness and the sample should not be exposed to any light before measurements are made: Since DCMU functions by displacing QB, even low light can cause quick net formation of Q –A artificially raising the measured F0 as Chl fluorescence is high when Q –A is present (Govindjee 2004; Huot and Babin Chapter 3).

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  1. Algae R&D Center, School of Biological Sciences and Biotechnology, Murdoch University, Murdoch, 6150, WA, Australia

    Jeff Cosgrove & Michael A. Borowitzka

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  1. , Department of Biological Sciences, University of Essex, Colchester, CO4 3SQ, United Kingdom

    David J. Suggett

  2. Institute of Microbiology, Opatovciky mlyn, Trebon, 379 81, Czech Republic

    Ondrej Prášil

  3. Murdoch University, School of Biological Sciences and Biotec, Algae R&D Center, Murdoch, 6150, West Australia, Australia

    Michael A. Borowitzka

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Cosgrove, J., Borowitzka, M.A. (2010). Chlorophyll Fluorescence Terminology: An Introduction. In: Suggett, D., Prášil, O., Borowitzka, M. (eds) Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications. Developments in Applied Phycology, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9268-7_1

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