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Abstract

Pancreatic islets are highly vascularized mini-organs that include endocrine cells, endothelial cells, and extracellular matrix. The three-dimensional islet structure and relationship between endocrine cells are different in rodent islets and human islets. This chapter reviews recent studies on the morphology, cell composition, and cell arrangement in human islets and how this may affect islet function.

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References

  1. Bonner-Weir S (1991) Anatomy of islet of Langerhans. In: Samols E (ed) The endocrine pancreas. Raven, New York, pp 15–27

    Google Scholar 

  2. Lifson N, Lassa CV, Dixit PK (1985) Relation between blood flow and morphology in islet organ of rat pancreas. Am J Physiol 249:E43–48

    PubMed  CAS  Google Scholar 

  3. Bonner-Weir S (1993) The microvasculature of the pancreas, with emphasis on that of the islets of Langerhans. In: Go VLW, DiMagno ER, Gardner JD, Lebenthal FP, Reber HA, Scheele GA (eds) The Pancreas: Biology, Pathobiology, and Disease, 2nd edn. Raven, New York, pp 759–768

    Google Scholar 

  4. Prado CL, Pugh-Bernard AE, Elghazi L, Sosa-Pineda B, Sussel L (2004) Ghrelin cells replace insulin-producing beta cells in two mouse models of pancreas development. Proc Natl Acad Sci USA 101:2924–2929

    Article  PubMed  CAS  Google Scholar 

  5. Heller RS, Jenny M, Collombat P, Mansouri A, Tomasetto C, Madsen OD, Mellitzer G, Gradwohl G, Serup P (2005) Genetic determinants of pancreatic epsilon-cell development. Dev Biol 286:217–224

    Article  PubMed  CAS  Google Scholar 

  6. Wierup N, Sundler F (2005) Ultrastructure of islet ghrelin cells in the human fetus. Cell Tissue Res 319:423–428

    Article  PubMed  Google Scholar 

  7. Wierup N, Yang S, McEvilly RJ, Mulder H, Sundler F (2004) Ghrelin is expressed in a novel endocrine cell type in developing rat islets and inhibits insulin secretion from INS-1 (832/13) cells. J Histochem Cytochem 52:301–310

    PubMed  CAS  Google Scholar 

  8. Wierup N, Svensson H, Mulder H, Sundler F (2002) The ghrelin cell: a novel developmentally regulated islet cell in the human pancreas. Regul Pept 107:63–69

    Article  PubMed  CAS  Google Scholar 

  9. Bauer GE (1988) Islets of Langerhans. In: Weiss L (ed) Cell and tissue biology, a textbook of histology. Urban & Schwarzenberg, Baltimore, pp 738–749

    Google Scholar 

  10. Masharani U, Karam JH, German MS (2001) Pancreatic hormones and diabetes mellitus. In: Greenspan FS, Gardner DG (eds) Basic and clinical endocrinology. Lange Medical Books/McGraw-Hill, New York, pp 658–746

    Google Scholar 

  11. Orci L, Baetens D, Rufener C, Amherdt M, Ravazzola M, Studer P, Malaisse-Lagae F, Unger RH (1976) Hypertrophy and hyperplasia of somatostatin-containing D-cells in diabetes. Proc Natl Acad Sci USA 73:1338–1342

    Article  PubMed  CAS  Google Scholar 

  12. Dubois M, Pattou F, Kerr-Conte J, Gmyr V, Vandewalle B, Desreumaux P, Auwerx J, Schoonjans K, Lefebvre J (2000) Expression of peroxisome proliferator-activated receptor gamma (PPARgamma) in normal human pancreatic islet cells. Diabetologia 43:1165–1169

    Article  PubMed  CAS  Google Scholar 

  13. Brissova M, Fowler MJ, Nicholson WE, Chu A, Hirshberg B, Harlan DM, Powers AC (2005) Assessment of human pancreatic islet architecture and composition by laser scanning confocal microscopy. J Histochem Cytochem 53:1087–1097

    Article  PubMed  CAS  Google Scholar 

  14. Cabrera O, Berman DM, Kenyon NS, Ricordi C, Berggren PO, Caicedo A (2006) The unique cytoarchitecture of human pancreatic islets has implications for islet cell function. Proc Natl Acad Sci USA 103:2334–2339

    Article  PubMed  CAS  Google Scholar 

  15. Brelje TC, Scharp DW, Sorenson RL (1989) Three-dimensional imaging of intact isolated islets of Langerhans with confocal microscopy. Diabetes 38:808–814

    Article  PubMed  CAS  Google Scholar 

  16. Redecker P, Seipelt A, Jorns A, Bargsten G, Grube D (1992) The microanatomy of canine islets of Langerhans: implications for intra-islet regulation. Anat Embryol (Berl) 185:131–141

    CAS  Google Scholar 

  17. Stefan Y, Orci L, Malaisse-Lagae F, Perrelet A, Patel Y, Unger RH (1982) Quantitation of endocrine cell content in the pancreas of nondiabetic and diabetic humans. Diabetes 31:694–700

    Article  PubMed  CAS  Google Scholar 

  18. Rahier J, Goebbels RM, Henquin JC (1983) Cellular composition of the human diabetic pancreas. Diabetologia 24:366–371

    Article  PubMed  CAS  Google Scholar 

  19. Clark A, Wells CA, Buley ID, Cruickshank JK, Vanhegan RI, Matthews DR, Cooper GJ, Holman RR, Turner RC (1988) Islet amyloid, increased A-cells, reduced B-cells and exocrine fibrosis: quantitative changes in the pancreas in type 2 diabetes. Diabetes Res 9:151–159

    PubMed  CAS  Google Scholar 

  20. Shapiro AM, Lakey JR, Ryan EA, Korbutt GS, Toth E, Warnock GL, Kneteman NM, Rajotte RV (2000) Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med 343:230–238

    Article  PubMed  CAS  Google Scholar 

  21. Street CN, Lakey JR, Shapiro AM, Imes S, Rajotte RV, Ryan EA, Lyon JG, Kin T, Avila J, Tsujimura T, Korbutt GS (2004) Islet graft assessment in the Edmonton Protocol: implications for predicting long-term clinical outcome. Diabetes 53:3107–3114

    Article  PubMed  CAS  Google Scholar 

  22. Sakuraba H, Mizukami H, Yagihashi N, Wada R, Hanyu C, Yagihashi S (2002) Reduced beta-cell mass and expression of oxidative stress-related DNA damage in the islet of Japanese Type II diabetic patients. Diabetologia 45:85–96

    Article  PubMed  CAS  Google Scholar 

  23. Baetens D, Malaisse-Lagae F, Perrelet A, Orci L (1979) Endocrine pancreas: three-dimensional reconstruction shows two types of islets of Langerhans. Science 206: 1323–1325

    Article  PubMed  CAS  Google Scholar 

  24. Malaisse-Lagae F, Stefan Y, Cox J, Perrelet A, Orci L (1979) Identification of a lobe in the adult human pancreas rich in pancreatic polypeptide. Diabetologia 17:361–365

    Article  PubMed  CAS  Google Scholar 

  25. Gersell DJ, Gingerich RL, Greider MH (1979) Regional distribution and concentration of pancreatic polypeptide in the human and canine pancreas. Diabetes 28:11–15

    Article  PubMed  CAS  Google Scholar 

  26. Orci L, Stefan Y, Malaisse-Lagae F, Perrelet A (1979) Instability of pancreatic endocrine cell populations throughout life. Lancet 1:615–616

    Article  PubMed  CAS  Google Scholar 

  27. Brissova M, Fowler MJ, Wiebe P, Shostak A, Shiota M, Radhika A, Lin PC, Gannon M, Powers AC (2004) Intra-islet endothelial cells contribute to revascularization of transplanted pancreatic islets. Diabetes 53:1318–1325

    Article  PubMed  CAS  Google Scholar 

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Brissova, M., Powers, A.C. (2008). Architecture of Pancreatic Islets. In: Seino, S., Bell, G.I. (eds) Pancreatic Beta Cell in Health and Disease. Springer, Tokyo. https://doi.org/10.1007/978-4-431-75452-7_1

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