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Cytochemistry of mature angiosperm pollen

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Pollen and Pollination

Abstract

The problems involved in applying histochemical and cytochemical methods to mature angiosperm pollen for bright light and fluorescence microscopy are discussed. These methods can be used for general examination or to reveal particular structures or groups of substances. The main methods of testing pollen viability and germinability based on stains and semiquantitative methods are also reviewed. The main methods of staining and their applications are summarised.

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Author information

Authors and Affiliations

  1. Department of Environmental Sciences, University of Siena, Via Mattioli 4, 1-53100, Siena, Italy

    M. Nepi

  2. Department of Pharmacology, University of Siena, Siena, Via Delle Scotte 6, I-53100, Italy

    G. G. Franchi

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  1. M. Nepi
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  2. G. G. Franchi
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Editors and Affiliations

  1. Laboratory of Pollination Ecology, Institute of Evolution, Haifa University, Haifa, Israel

    Amots Dafni

  2. Institut für Botanik und Botanischer Garten, Universität Wien, Wien, Austria

    Michael Hesse

  3. Dipartimento di Biologia Ambientale, Università degli Studi di Siena, Siena, Italy

    Ettore Pacini

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Nepi, M., Franchi, G.G. (2000). Cytochemistry of mature angiosperm pollen. In: Dafni, A., Hesse, M., Pacini, E. (eds) Pollen and Pollination. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6306-1_3

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  • DOI: https://doi.org/10.1007/978-3-7091-6306-1_3

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