Abstract
Role of carbon monoxide (CO) in hyperthermic brain injury induced brain pathology was examined in a rat model using immunohistochemistry of the hemeoxygenase-2 (HO-2) enzyme. Exposure of rats to 4 h heat stress at 38°C resulted in profound hyperthermia, breakdown of the blood-brain barrier (BBB), brain edema formation, cell damage and expression of HO-2 in several brain regions. Pretreatment with potent antioxidant compounds EGB-761 and BN-52021 markedly reduced the HO-2 expression, BBB breakdown, brain edema formation and cell damage without attenuating the hyperthermic response. This effect was most marked in animals treated with EGB-761. These observations suggest that upregulation of HO-2 representing generation of CO plays important roles in hyperthermic brain injury, and oxidative stress seems to be one of the most important signals in inducing HO-2 expression in hyperthermia, not reported earlier.
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References
Bastianetto S, Zheng WH, Quirion R (2000)The Gingko biloba extract (EGb 761) protects and rescues hippocampal cells against nitric oxide-induced toxicity: involvement of its flavonoid constituents and protein kinase C. J Neurochem 74: 2268–2277
Drieu K, Vrancks R, Benassayad C, Haourigi M, Hassid J, Yoa RG, Rapin JR, Nunez EA (2000) Effect of the extract of gingko biloba (EGB 761) on the circulating and cellular profiles of polyunsaturated fatty acids: correlation with the anti-oxidant properties of the extract. Prostaglandins Leukot Essential Fatty Acids 63: 293–300
Flanagan SW, Mooseley PL, Buettner GR (1998) Increased flux of free radicals in cells subjected to hyperthermia: detection by electron paramagnetic resonance spin trapping. FEBS Lett 431: 285–286
Freeman ML, Spitz DR, Meredith MJ (1990) Does heat shock enhance oxidative stress? Studies with ferrous and ferric iron. Radiat Res 124: 288–293
Hedlund S, Sharma HS, Sjoquist P-O, Westman J (1999) A new antioxidant compound H-290/51 attenuates heat shock protein (HSP 72 kD) response, edema and cell injury following acute heat exposure. An experimental study using light and electron microscopy in the rat. J Therm Biol 24: 409–414
Kim CK, Rivier CL (2000) Nitric oxide and carbon monoxide have a stimulatory role in the hypothalamic-pituitary-adrenal response to physico-emotional stressors in rats. Endocrinology 141: 2244–2253
Maines MD (1997) The heme oxygenase system: a regulator of second messengergases. Annu Rev Pharmacol Toxicol 37: 517–554
Miura T, Muraoka S, Fujimoto Y (1998) Temperature-dependent lipid peroxidation of rat brain homogenate. Res Commun Mol Pathol Pharmacol 100: 117–128
Pietri S, Seguin JR, d’Arbigny P, Drieu K, Culcasi M (1997) Gingko biloba extract (EGb 761) pretreatment limits free radical-induced oxidative stress in patients undergoing coronary bypass surgery. Cardiovasc Drugs Ther 11: 121–131
Sastre J, Millan A, Garcia de la Asuncion J, Pia R, Juan G, Pallardo, O’Conner E, Martin JA, Droy-Lefaix MT, Vina J (1998) A Gingko biloba extracts (EGb 761) prevents mitochondrial aging by protecting against oxidative stress. Free Radic Biol Med 24: 298–304
Sharma HS, Dey PK (1986) Probable involvement of 5-hydroxytryptamine in increased permeability of blood-brain barrier under heat stress. Neuropharmacology 25: 161–167
Sharma HS, Dey PK (1987) Influence of long-term acute heat exposure on regional blood-brain barrier permeability, cerebral blood flow and 5-HT level in conscious normotensive young rats. Brain Res 424: 153–162
Sharma HS, Aim P, Westman J (1997a) Upregulation of hemeoxygenase-II in the rat spinal cord following heat stress. Thermal physiology. In: Nielsen-Johanssen B, Nielsen R (eds) The August Krogh Institute, Copenhagen, 1997, pp 135–138
Sharma HS, Westman J, Cervos-Navarro J, Nyberg F (1997b) Role of neurochemicals in brain edema and cell changes following hyperthermic brain injury in the rat. Acta Neurochir (Wien) [Suppl] 70: 269–274
Sharma HS, Aim P, Westman (1998a) Nitric oxide and carbon monoxide in the pathophysiology of brain functions in heat stress. Prog Brain Res 115: 297–333
Sharma HS, Westman J, Nyberg F (1998b) Pathophysiology of brain edema and cell changes following hyperthermic brain injury. Prog Brain Res 115: 351–412
Sharma HS (1999) Pathophysiology of blood-brain barrier, brain edema and cell injury following hyperthermia: New role of heat shock protein, nitric oxide and carbon monoxide. An experimental study in the rat using light and electron microscopy. Acta Universitatis Upsaliensis 830: 1–94
Sharma HS, Drieu K, Aim P, Westman J (1999) Upregulation of neuronal nitric oxide synthase, edema and cell injury following heat stress are reduced by pretreatment with EGB-761 in the rat. J Therm Biol 24: 439–446
Sharma HS, Drieu K, Aim P, Westman J (2000a) Role of nitric oxide in the blood-brain barrier permeability, brain edema and cell damage following hyperthermic brain injury. An experimental study using EGB-761 and Gingkolide B pretreatment in the rat. Acta Neurochir (Wien) [Suppl] 76: 81–86
Sharma HS, Aim P, Sjoquist P-O, Westman J (2000b) A new antioxidant compound H-290/51 attenuates upregulation of constitutive isoform of heme oxygenase (HO-2) following trauma to the rat spinal cord. Acta Neurochir (Wien) [Suppl] 76: 153–157
Snyder SH, Ferris CD (2000) Novel neurotransmitters and their neuropsychiatric relevance. Am J Psychiatry 157: 1738–1751
Westman J, Drieu K, Sharma HS (2000) Antioxidant compounds EGB-761 and BN-52021 attenuate heat shock protein (HSP 72 kD) response, edema and cell changes following hyperthermic brain injury. An experimental study using immunohistochemistry in the rat. AminoAcids 19: 339–350
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© 2003 Springer-Verlag Wien
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Sharma, H.S., Drieu, K., Westman, J. (2003). Antioxidant compounds EGB-761 and BN-52021 attenuate brain edema formation and hemeoxygenase expression following hyperthermic brain injury in the rat. In: Kuroiwa, T., et al. Brain Edema XII. Acta Neurochirurgica Supplements, vol 86. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0651-8_68
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DOI: https://doi.org/10.1007/978-3-7091-0651-8_68
Publisher Name: Springer, Vienna
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