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The Canonical RNA Interference Pathway in Animals

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Regulatory RNAs

Abstract

The canonical RNA interference (RNAi) pathway is defined as a sequence-specific mRNA degradation mediated by short RNA molecules which are generated from long double-stranded RNA. Since its discovery in 1998, RNAi has become a popular tool for experimental silencing of gene expression. On the other hand, its natural role received less attention. Recent studies in animal systems, particularly the use of the next generation sequencing and analysis of animals defective in some aspect of small RNA biogenesis, revealed novel functions of RNAi and cross talks between RNAi and other pathways employing small RNAs. This chapter provides a comprehensive view of the natural canonical RNAi pathway in animals including its molecular mechanism and different biological roles.

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Acknowledgments

We thank Radek Malik for help with manuscript preparation. Our research is supported by the following grants: EMBO SDIG project 1483, GACR 204/09/0085, GACR P305/10/2215, and Kontakt ME09039. P.S. is a holder of the J.E. Purkyne Fellowship.

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  1. Institute of Molecular Genetics AS CR, Prague, Czech Republic

    Jana Nejepinska, Matyas Flemr & Petr Svoboda

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  1. Jana Nejepinska
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    Bibekanand Mallick

  2. A.J.C. Bose Centenary Building, P-1/12, CIT Scheme - VII M, Bose Institute, Kolkata, 700054, India

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Nejepinska, J., Flemr, M., Svoboda, P. (2012). The Canonical RNA Interference Pathway in Animals. In: Mallick, B., Ghosh, Z. (eds) Regulatory RNAs. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22517-8_5

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