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Saliva Collection Devices and Diagnostic Platforms

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Advances in Salivary Diagnostics

Abstract

Over the last few years, there has been a tremendous growth in less invasive diagnostic testing as a replacement for painful and expensive blood draws. The role of saliva in this growth trend has been enormous, and this has been driven in part by a growing awareness of the broad utility of saliva as a diagnostic medium and reinforced by a rapidly growing number of publications supporting new and varied applications for saliva. The aim of this chapter is to highlight some of the tools now available that are responsible for this growth trend and provide a glimpse into the future for salivary diagnostics in the research and clinical environments.

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Notes

  1. 1.

    Definition from Jablonski S. Illustrated Dictionary of Dentistry, W B Saunders Co. July 1982.

References

  1. NIH Public Health Service Document “Healthy People 2010”. Available at: http://www.healthy.gov/healthypeople/. Accessed 31 May 2014.

  2. NIH Budget Proposal for the NIDCR 2006 Lawrence A Tabak DDS, PhD: Presentation to the House Sub-Committee March 9th 2005.

    Google Scholar 

  3. Tabak LA. The revolution in biomedical assessment: the development of salivary diagnostics. J Dent Educ. 2001;65(12):1335–9.

    Google Scholar 

  4. American Dental Association Position Statement on Oral Fluid Diagnostics. Available at: http://www.ada.org/prof/resources/positions/statements/fluid_diagnostics.asp. Accessed 31 May 2014.

  5. International Association of Dental Research [IADR]. http://www.iadr.com/i4a/search.cfm#.UqTeSfRDtrp. Accessed 31 May 2014.

  6. FDI Policy Statement on Salivary Diagnostics, Adopted by the General Assembly, Istanbul, Aug 2013. Accessible at: http://www.fdiworlddental.org/media/31116/salivary_diagnostics-2013.pdf. Accessed 31 May 2014.

  7. Hu S, Li Y, Wang J, Xie Y, Tjon K, Wolinsky L, Loo RRO, Loo JA, Wong DT, et al. Human salivary proteome and transcriptome. J Dent Res. 2006;85:1129–33.

    Article  PubMed Central  PubMed  Google Scholar 

  8. Streckfus CF, Bigler L, Tucci M, Thigpen JT. A Preliminary Study of CA 15-3, c-erbB-2, Epidermal growth factor, Cathepsin D and p53 in saliva of women with breast carcinoma. Cancer Invest. 2000;18(2):101–9.

    Article  PubMed  Google Scholar 

  9. Streckfus CF, Bigler L. The use of soluble, salivary c-erb-b2 for the detection and post-operative follow-up of breast cancer in women: the results of a five-year translational research study. Adv Dent Res. 2005;18(1):17–24.

    Article  PubMed  Google Scholar 

  10. Streckfus CF, Bigler L, Dellinger TD, Dai X, Kingman A, Thigpen JT. The presence of soluble c-erbB-2 in saliva and serum of women with breast carcinoma: a preliminary study. Clin Cancer Res. 2000;6(6):2363–70.

    PubMed  Google Scholar 

  11. Oral Diagnostics Symposium, Lanier Lakes [Atlanta] 2006. Oral-based diagnostics. Ann N Y Acad Sci. 2007;1098.

    Google Scholar 

  12. Boston Business Journal Bioflash, March 18th 2013. Available at: http://www.bizjournals.com/boston/blog/bioflash/2013/03/state-gives-41m-grant-to-build.html. Accessed 31 May 2014.

  13. Malamud, Rodriguez-Chavez IR. Saliva as a diagnostic fluid. Dent Clin North Am. 2011;55(1):159–78.

    Article  PubMed Central  PubMed  Google Scholar 

  14. Punyadeera C, Slowey PD. Chapter 22: Saliva as an emerging biofluid for clinical diagnosis and applications of MEMS/NEMS in salivary diagnostics. In: Subramani K, Ahmed A, Hartsfield JK, Eds. Nanobiomaterials in clinical dentistry. New York: Elsevier; 2013. p. 453–75. ISBN 978-1-4557-3127-5.

    Google Scholar 

  15. Streckfus C, Bigler L. Saliva as a diagnostic fluid. Oral Dis. 2002;8:69–76.

    Article  PubMed  Google Scholar 

  16. Slowey PD. Commercial saliva collections tools. J Calif Dent. 2013;41(2):97–105.

    Google Scholar 

  17. Malamud D, Tabak LA, editors. Saliva as a diagnostic fluid, vol. 694. New York: Annals of New York Academy of Sciences; 1992.

    Google Scholar 

  18. Samaranayake LP. Saliva tells the body’s health. In: Moss S, editor. The benefits of chewing. 2006. p. 24–35.

    Google Scholar 

  19. Rollins G. Saliva tests: ready to spit? Clinical Laboratory News. 2008;34(8).

    Google Scholar 

  20. Hofman LF. Human saliva as a diagnostic specimen. Am Soc Nutr Sci. 2001;131:1621S–5.

    Google Scholar 

  21. Punyadeera C. An often forgotten, but convenient diagnostic fluid. Clinical Laboratory News. 2013;39(1).

    Google Scholar 

  22. Why Your Saliva May be the Real Reason You Have Digestive Issues. http://taoofmedicine.com/category/mind-body-medicine/. Accessed 31 May 2014.

  23. Mandel ID. A contemporary view of salivary research. Clin Rev Oral Biol Med. 1993;4:599.

    Google Scholar 

  24. Kozaki T, Lee S, Nishimura T, Katsuura T, Yasukouchi A. Effects of saliva collection using cotton swabs on melatonin enzyme immunoassay. J Circadian Rhythms. 2011;9:1.

    Article  PubMed Central  PubMed  Google Scholar 

  25. Gleeson M, Li T-L. The cotton swab method for human saliva collection: effect on measurements of saliva flow rate and concentration of protein, secretory immunoglobulin A, amylase and cortisol. In: Proceedings of the Physiological Society, Human and Exercise Physiology abstracts, University College London. J Physiol. 2003;547P, PC5.

    Google Scholar 

  26. Mohamed R, Campbell J-L, Cooper White J, Dimeski G, Punyadeera C. The impact of saliva collection and processing methods on CRP, IgE and myoglobin immunoassays. Clin Transl Med. 2012;1:19.

    Article  PubMed Central  PubMed  Google Scholar 

  27. Shirtcliff EA, Granger DA, Schwartz E, Curran MJ. Use of salivary biomarkers in behavioral research: cotton-based sample collection methods can interfere with salivary immunoassay results. Psychoneuroendocrinology. 2001;26:165–73.

    Article  PubMed  Google Scholar 

  28. Frost and Sullivan report: globalization of IVD companies, summarized in Genetic Engineering News. 1 May 2013;33(9). Available at http://www.slideshare.net/FrostandSullivan/the-globalization-of-in-vitro-diagnostics-markets. Accessed 31 May 2014.

  29. http://newsroom.ucla.edu/portal/ucla/ucla-dentistry-receives-5-million246826.aspx. Accessed 31 May 2014.

  30. Mese H, Matsuo R. Salivary secretion, taste and hyposalivation. J Oral Rehabil. 2007;34:711–23.

    Article  PubMed  Google Scholar 

  31. Wolff A, Davis RL. Universal collector for submandibular and sublingual saliva. US Patent Number 5,050,616.

    Google Scholar 

  32. Wolff A, Begleiter A, Moskona D. A novel system of human submandibular and sublingual saliva collection. J Dent Res. 1997;76(11):1782–6.

    Article  PubMed  Google Scholar 

  33. Morales I, Dominguez P, López RO. Devices for saliva collection from the major salivary glands. Results in normal subjects. Rev Med Chil. 1998;126(5):538–47.

    PubMed  Google Scholar 

  34. Morales I, Urzūa-Orellana B, Aguilera S, Dominguez P, López RO. Patterns and variability of in electrophoretic polypeptide profiles of human saliva in a healthy population. J Physiol Biochem. 2006;62(3):179–88.

    Article  Google Scholar 

  35. Morales I, Urzūa-Orellana B, Dominguez P, Retamales P. Available at: http://www.captura.uchile.cl/bitstream/handle/2250/7027/MoralesB_Irene.pdf?sequence=1. Accessed 31 May 2014.

  36. Hanning S, Motio L, Medlicott NJ, Swindells S. A device for the collection of submandibular saliva. N Z Dent J. 2012;108(1):4–8.

    PubMed  Google Scholar 

  37. Bauman R, DeBoer J. A modification of the altered cast technique. J Prosthet Dent. 1982;47:212–3.

    Article  PubMed  Google Scholar 

  38. Nederfors T, Dahlöf C. A modified device for collection and flow rate measurement of submandibular and sublingual saliva. Eur J Oral Sci. 1993;101(4):210–4.

    Article  Google Scholar 

  39. Block P, Brotman SA. A method of submaxillary saliva without cannulization. N Y State Dent J. 1962;28:116–9.

    Google Scholar 

  40. Wong DT. Salivary diagnostics. Ames: Wiley-Blackwell; 2008. ISBN 978-0-8138-1333-2.

    Google Scholar 

  41. Jiang J, Park NJ, Hu S, Wong DT. A universal pre-analytic solution for concurrent stabilization of salivary proteins, RNA and DNA at ambient temperature. Arch Oral Biol. 2009;54:268–73.

    Article  PubMed Central  PubMed  Google Scholar 

  42. Park NJ, Yu T, Nabili V, Brinkman BMN, Henry S, Wang J, Wong DT. RNAprotect saliva: an optimal room temperature stabilization reagent for the salivary transcriptome. Clin Chem. 2006;52(12):2303–4.

    Article  PubMed  Google Scholar 

  43. Kurth A. Potential biohazard risk from infectious tissue and culture cells preserved with RNAlater™. Clin Chem. 2007;53(7):1389–90.

    Article  PubMed  Google Scholar 

  44. http://www.invitek.de/products_and_service/products. Accessed 31 May 2014.

  45. http://www.norgenbiotek.com/display-product.php?ID=385. Accessed 31 May 2014.

  46. http://www.salimetrics.com/articles/print/collecting-and-handling-saliva-for-dna-samples. Accessed 31 May 2014.

  47. Thomadaki K, Helmerhorst EJ, Tian N, Sun X, Siqueira WL, Walt DR, Oppenheim FG. Whole-saliva proteolysis and its impact on salivary diagnostics. J Dent Res. 2011;90(11):1325–30.

    Article  PubMed Central  PubMed  Google Scholar 

  48. Xiao H, Wong DT. Proteomic analysis of microvesicles in human saliva by gel electrophoresis with liquid chromatography-mass spectrometry. Anal Chim Acta. 2012;722:63–9.

    Article  PubMed  Google Scholar 

  49. Esser D, Alvarez-Llamas G, Dr Vries MP, Weening D, Vonk RJ, Roelofsen H. Sample stability and protein composition of saliva: implications for its use as a diagnostic fluid. Biomark Insights. 2008;3:25–37.

    PubMed Central  PubMed  Google Scholar 

  50. Warrener L, Slibinskas R, Chua KB, Brown KE, Nigatu W, Sasnauskas K, Samuel D, Brown D. A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid. Bull World Health Organ. 2011;89:675–82.

    Article  PubMed Central  PubMed  Google Scholar 

  51. Punyadeera C, Domeski G, Kostner K, Beyerlein P, Cooper-White J. One-step homogeneous C-reactive protein assay for saliva. J Immunol Methods. 2011;373:19–25.

    Article  PubMed  Google Scholar 

  52. Lane J, Flint J, Danks C. The development of a rapid diagnostic test for cortisol in the saliva of pigs. Report for the UK Government Department of the Environment Food and Rural Affairs [DEFRA]; London, England: 2010.

    Google Scholar 

  53. NIH Awarded Grant [NCCAM, 2R44AT006634-04]. VerOFy®, a new tool to improve productivity and reduce costs for stress research. Oasis Diagnostics Corporation, Aug 2013.

    Google Scholar 

  54. Weigum SE, Floriano PN, Cristodoulides N, McDevitt JT. Toward the development of a lab-on-a-chip dual function leucocyte and C-reactive protein analysis method for the assessment of inflammation and cardiac risk. Lab-on-a-Chip. 2007;7:995–1003.

    Article  PubMed  Google Scholar 

  55. Stevens RC, Soelberg SD, Near S, Furlong CE. Detection of cortisol in saliva with a flow-filtered, portable surface resonance biosensor system. Anal Chem. 2008;80(17):6747–51.

    Article  PubMed Central  PubMed  Google Scholar 

  56. Moore C, Kelley-Baker T, Lacey J. Field testing of the Alere DDS2 mobile test system for drugs in oral fluid. J Anal Toxicol. 2013;37:305–7.

    Article  PubMed  Google Scholar 

  57. Molecular Diagnostics Market Report 2009 Research and Markets Limited Report. Available at: http://www.researchandmarkets.com/product/65f2db/molecular_diagnostics_markets. Accessed 31 May 2014.

  58. Kalorama Information Market Report: World Molecular Diagnostics Market Update. Available at: http://www.kalroamainformation.com/Molecular-Diagnostics-Month-7969379/. Accessed 31 May 2014.

  59. Gallo A, Tandon M, Alevizos I, Illie GG. The majority of microRNAs detectable in serum and saliva is concentrated in exosomes. PLoS One. 2012;7(3):e30679. www.Plosone.org.

  60. Bahlo M, Stankovich J, Danoy P, Hickey PF, Taylor BV, Browning SR, et al. Saliva-derived DNA performs well in large-scale, high-density, single-nucleotide polymorphism microarray studies. Cancer Epidemiol Biomarkers Prev. 2010;19:794–8.

    Article  PubMed  Google Scholar 

  61. Abraham JE, Maranian MJ, Spiteri I, Russell R, Ingle S, Luccarini C, et al. Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping. BMC Genomics. 2012;5(19):1–6.

    Google Scholar 

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Correspondence to Paul Desmond Slowey BSc (Hons), PhD .

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Slowey, P.D. (2015). Saliva Collection Devices and Diagnostic Platforms. In: Streckfus, C. (eds) Advances in Salivary Diagnostics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-45399-5_3

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  • DOI: https://doi.org/10.1007/978-3-662-45399-5_3

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