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In Vitro Testing in Contact Hypersensitivity

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Textbook of Contact Dermatitis

Abstract

The development of testing procedures to aid in the diagnosis of allergic contact dermatitis began with the introduction of the concept of patch testing by Jadassohn [1]. This involved reproducing allergic eczema, following application of antigen to a small area of unbroken skin in a sensitized individual. Almost a century later the technique, despite its empirical basis, has with refining and standardization developed into a useful tool in the investigation of contact dermatitis. Patch testing, however, gives rise to a number of problems. Interpretation is subjective and relies on observer experience. False-positive and false-negative reactions can occur [2–4]. In addition, the procedure is not without possible risks. In particular, patch testing may cause active sensitization of previously nonallergic patients.

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Authors
  1. Clifford McMillan
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  2. Desmond Burrows
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Editors and Affiliations

  1. St. John’s Institute of Dermatology, St. Thomas’s Hospital, SE1 7EH, London, UK

    Richard J. G. Rycroft

  2. Department of Dermatology, Gentofte Hospital, University of Copenhagen, Niels Andersens Vej 65, 2900, Hellerup, Denmark

    Torkil Menné

  3. Hautklinik der Städtischen Kliniken Dortmund, Abteilung Dermatologie, Universität Witten/Herdecke, Beurhausstraße 40, 44123, Dortmund, Germany

    Peter J. Frosch

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McMillan, C., Burrows, D. (1995). In Vitro Testing in Contact Hypersensitivity. In: Rycroft, R.J.G., Menné, T., Frosch, P.J. (eds) Textbook of Contact Dermatitis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03104-9_18

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  • DOI: https://doi.org/10.1007/978-3-662-03104-9_18

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