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In Vivo Labeling of DNA Probes with 5-BrdU

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Nonradioactive Labeling and Detection of Biomolecules

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Abstract

The thymidine analogue 5-bromodeoxyuridine (BrdU) can specifically and quantitatively replace thymidine in DNA molecules using either an in vivo (Dunn and Smith, 1954; Zamenhof and Griboff, 1954; Hackett and Hanawalt, 1966) or in vitro (Bessman et al., 1958) enzymatic reactions.

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Authors
  1. Jean-Luc Guesdon
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Editors and Affiliations

  1. Forschungszentrum Penzberg, Boehringer Mannheim GmbH, Nonnenwald 2, W-8122, Penzberg, Germany

    Christoph Kessler

  2. Institut für Biochemie, Ludwig-Maximilians-Universität München, Karlstr. 21, W-8000, München 2, Germany

    Christoph Kessler

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© 1992 Springer-Verlag Berlin Heidelberg

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Guesdon, JL. (1992). In Vivo Labeling of DNA Probes with 5-BrdU. In: Kessler, C. (eds) Nonradioactive Labeling and Detection of Biomolecules. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00144-8_5

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  • DOI: https://doi.org/10.1007/978-3-662-00144-8_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-00146-2

  • Online ISBN: 978-3-662-00144-8

  • eBook Packages: Springer Book Archive

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