Skip to main content
SpringerLink
Log in

The Mode of Action of Soybean Trypsin Inhibitor as Revealed by Crystal Structure Analysis of the Complex with Porcine Trypsin

  • Conference paper
Proteinase Inhibitors

Part of the book series: Bayer-Symposium ((BAYER-SYMP,volume 5))

Abstract

In this paper we would like to accomplish four things:

  1. 1.

    To describe in a general way, and then in some detail, how the soybean trypsin inhibitor (Kunitz) (STI) binds to porcine trypsin, based on X-ray diffraction results to 2.6 Å resolution.

  2. 2.

    To show the evidence from which we deduce the structure of the complex at the active site.

  3. 3.

    To explain why this might be a more stable form for the complex than other alternatives.

  4. 4.

    To compare this complex to that of bovine trypsin with the basic pancreatic trypsin inhibitor (Kunitz) [1,2] (PTI).

Supported by Damon Runyon Memorial Fund and the European Molecular Biology Organisation

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ruhlman, A., Kukla, D., Schwager, P., Bartels, K., Huber, R.: J. molec. Biol. 77, 417–436 (1973).

    Article  Google Scholar 

  2. Huber, R., et al.: This volume, p. 497.

    Google Scholar 

  3. Stroud, R.M, Kay, L.M., Dickerson, R.E.: Cold Spr. Harb. Symp. quant. Biol. 36, 125–140 (1971).

    Article  CAS  Google Scholar 

  4. Hermodson, M.A., Ericsson, L.H., Neurath, H., Walsh, K.A.: Biochemistry 12, 3146–3153 (1973).

    Article  PubMed  CAS  Google Scholar 

  5. Koide, T., Ikenaka, T.: Europ. J. Biochem. 32, 417–431 (1973).

    Article  PubMed  CAS  Google Scholar 

  6. Neil, G.L., Niemann, C., Hein, G.E.: Nature (Lond.) 210, 903 (1966).

    Article  CAS  Google Scholar 

  7. Blow, D. M.: This volume, p.473.

    Google Scholar 

  8. Diamond, R.: Acta Cryst. A 27, 436 (1971).

    Article  CAS  Google Scholar 

  9. Private communication from R. HUBER.

    Google Scholar 

Download references

Author information

Author notes

  1. J. Janin

    Present address: Service de Biochimie Cellulaire, Institut Pasteur, Paris, France

  2. R. M. Sweet

    Present address: Department of Chemistry, University of California, Los Angeles, CA, USA

Authors and Affiliations

  1. Medical Research Council Laboratory of Molecular Biology, Cambridge, Great Britain

    J. Janin, R. M. Sweet & D. M. Blow

Authors
  1. J. Janin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. M. Sweet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. M. Blow
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Klinische Chemie und Klinische Biochemie, Universität München, 8000, Munich 2, Fed. Rep. Germany

    Hanz Fritz

  2. Organisch-Chemisches Laboratorium, Technischen Universität München, 8000, Munich 2, Fed. Rep. Germany

    Harald Tschesche

  3. Department of Biology, Brookhaven National Laboratory, Upton, NY, 11973, USA

    Lewis J. Greene

  4. Biochemisches Laboratorium, Bayer AG, 5600, Wuppertal 1, Fed. Rep. Germany

    Ernst Truscheit (Editor-in-Chief) (Editor-in-Chief)

Rights and permissions

Reprints and permissions

Copyright information

© 1974 Springer-Verlag

About this paper

Cite this paper

Janin, J., Sweet, R.M., Blow, D.M. (1974). The Mode of Action of Soybean Trypsin Inhibitor as Revealed by Crystal Structure Analysis of the Complex with Porcine Trypsin. In: Fritz, H., Tschesche, H., Greene, L.J., Truscheit, E. (eds) Proteinase Inhibitors. Bayer-Symposium, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87966-1_56

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-87966-1_56

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-87968-5

  • Online ISBN: 978-3-642-87966-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation