Abstract
Rabbits immunized with complexes of methylated bovine serum albumin (MBSA) and denatured DNA produce antibodies directed toward DNA (Plescia et al., 1964). These antibodies, like those described for the glucosylated DNA of the T-even phages (Levine et al., 1960) react with the denatured antigen; a conformation in which the bases are not involved in hydrogen bonding and are therefore available for interaction with the antibody. The antibodies to the T-even phage DNA’s are highly specific for the glucosylated cytosine moieties (Murakami et al., 1961; Townsend et al., 1965) and can be used to determine the extent of phage DNA synthesis in T4-infected E. coli cells even though host cell DNA is present in large excess (Levine et al., 1960).
Aided by grants from the NIH (AI-01940 and AI-02792) and the Charles Simon Memorial Grant for Cancer Research from the American Cancer Society (E.222). L. L. is an American Cancer Society Professor of Biochemistry and H. V. V. is a recipient of a Public Health Service Research Career Award K6-AI-2372. Publication No. 565 from the Graduate Department of Biochemistry, Brandeis University.
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Seaman, E., Levine, L., Van Vunakis, H. (1968). Immunological Evidence for the Existence of Thymine Dimers in Ultraviolet Irradiated DNA. In: Plescia, O.J., Braun, W. (eds) Nucleic Acids in Immunology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-87668-4_12
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