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Analytical Studies of Dye-Coupling between Plant Cells

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Parallels in Cell to Cell Junctions in Plants and Animals

Part of the book series: NATO ASI Series ((ASIH,volume 46))

Abstract

Cell-to-cell communication, defined as the regulated diffusion of ions, metabolites, and other informational molecules through the plasmodesmata, was first suggested by Tangl in 1879. The process allows for the distribution of metabolites throughout a plant and is required for the proper growth and development of plants. Renewed interest in this topic — 100 years after its proposal — has resulted in several reviews (Spanswick, 1975; Gunning and Robards, 1976; Gunning and Overall, 1983; Goodwin and Erwee, 1985). To understand this phenomenon our approach has been (1) to study the ultrastructure of plasmodesma; (2) to determine which plant cells are coupled by using dye passage studies; (3) to analyze the kinetics of cell-to-cell diffusion; (4) to examine the regulation of cell-to-cell communication; and (5) to observe changes in whole plant growth and development when cell-to-cell coupling has been altered. The tissue we use to study the kinetics and regulation of cell-to-cell transport is staminal hairs of Setcreasea purpurea, because such tissue provide a system in which symplastic cell to cell passage can be quantitatively measured. The tissue used to study growth and development is Onoclea sensibilis (fern) prothallia.

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Author information

Authors and Affiliations

  1. Department of Natural Sciences, Baruch College City University of New York, 17 Lexington Avenue, New York, NY, 10010, USA

    Edward B. Tucker

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  1. Edward B. Tucker
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Editor information

Editors and Affiliations

  1. Institute for Applied Biology, The University of York, Y01 5DD, York, UK

    A. W. Robards

  2. Department of Botany, University of California, 95616, Davis, CA, USA

    W. J. Lucas

  3. Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, G61 1BD, Bearsden, Glasgow, UK

    J. D. Pitts

  4. Department of Physiology, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands

    H. J. Jongsma

  5. Department of Neuroscience, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave, 10461, Bronx, NY, USA

    D. C. Spray

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© 1990 Springer-Verlag Berlin Heidelberg

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Tucker, E.B. (1990). Analytical Studies of Dye-Coupling between Plant Cells. In: Robards, A.W., Lucas, W.J., Pitts, J.D., Jongsma, H.J., Spray, D.C. (eds) Parallels in Cell to Cell Junctions in Plants and Animals. NATO ASI Series, vol 46. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83971-9_16

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  • DOI: https://doi.org/10.1007/978-3-642-83971-9_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-83973-3

  • Online ISBN: 978-3-642-83971-9

  • eBook Packages: Springer Book Archive

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