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Electrophoretic Karyotyping of Yeasts

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Nonconventional Yeasts in Biotechnology

Abstract

Electrophoretic karyotyping means the separation of intact chromosomal DNA according to its size on an agarose gel. Depending on the number and size of the chromosomes present in a strain, a specific banding pattern will be obtained. In order to reach this goal, two demands must be met. First, it is important to prepare the DNA without degradation by mechanical stress or by DNAses. Second, a method for the electrophoretic separation of the extremely large molecules must be developed. Conventional DNA electrophoresis is able to separate molecules of up to 50 kilobases. Yeast chromosomes range from several hundred to several thousand kilobases.

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Authors and Affiliations

  1. Institut für Biologie IV (Mikrobiologie) der Rheinisch-Westfälischen Technischen Hochschule Aachen, Worringer Weg, 52056, Aachen, Germany

    Martin Zimmermann

  2. Centre de Biotechnologies Agro-Industrielles, Laboratoire de Génétique Moléculaire et Cellulaire, Institut National de la Recherche Agronomique, 78850, Thiverval-Grignon, France

    Philippe Fournier

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© 1996 Springer-Verlag Berlin Heidelberg

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Zimmermann, M., Fournier, P. (1996). Electrophoretic Karyotyping of Yeasts. In: Nonconventional Yeasts in Biotechnology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79856-6_3

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  • DOI: https://doi.org/10.1007/978-3-642-79856-6_3

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  • Print ISBN: 978-3-642-79858-0

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