Abstract
Microbial ecosystems contain a large diversity of bacterial species. They are dominated by complex interactions between the different microorganisms, whereby each of the individual species has a specific role in the maintenance of the system. The active communities can efficiently scavenge nutrients from the environment and eliminate toxic compounds. However, not all of the species are active; most of them are dormant until environmental conditions change to favour their growth. Because of the great metabolic diversity and flexible organisation microbial ecosystems can be found nearly everywhere. Examples are the bacterial biofilms in waste water treatment reactors, on ship walls, or the microbial mats, found in hypersaline environments, tidal sediments and hot springs.
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References
Amann RI, Binder B. Chisholm SW, Olsen R, Devereux R, Stahl DA (1990) Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56: 1919–1925
Corstjens P, Muyzer G (1993) Phylogenetic analysis of the metal-oxidizing bacteria Leptothrix discophora and Sphaerotilus natans using 16S rDNA sequencing data. System Appl Microbiol 16: 219–223
Furhman JA, McCallum K, Davis AA (1992) Novel major archaebacterial group from marine plankton. Nature 356: 148–149
Giovannoni SJ (1991) The polymerase chain reaction. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. John Wiley and sons Ltd, Chichester, pp 177–203
Giovannoni SJ, DeLong EF, Olsen GJ, Pace NR (1990) Genetic diversity in Sargasso Sea bacterioplankton. Nature 345: 60–63
Höfle M (1992) Bacterioplankton community structure and dynamics after large-scale release of nonindignous bacteria as revealed by low-molecular-weight-RNA analysis. Appl Environ Microbiol 58
Huber R, Stetter KO (1992) The order Thermotogales. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleiffer K-H (eds) The Prokaryotes 2nd edition, pp 3809–3815
Lee S, Fuhrman JA (1990) DNA hybridization to compare species compositions of natural bacterioplankton assemblages. Appl Environ Microbiol 56: 739–746
Lee S, Furhman JA (1991) Spatial and temporal variation of natural bacterioplankton assemblages studied by total genomic DNA cross-hybridization. Limnol Oceanogr 36: 1277–1287
Lerman LS, Fischer SG, Hurley I, Silverstein K, Lumelsky N (1984) Sequence-determined DNA separations. Annu Rev Biophys Bioeng 13: 399–423
Liesack W, Weyland H, Stackebrandt E (1991) Potential risks of gene amplification by PCR as determined by 16S rDNA analysis of a mixed-culture of strict barophilic bacteria. Microb Ecol 21: 191–198
Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59: 695–700
Neefs J-M, van de Peer Y, Hendriks L, de Wachter R (1990) Compilation of small ribosomal subunit RNA sequences. Nucleic Acid Res 18: 2237–2317
Olsen GJ, Larses N, Woese CR (1991) The ribosomal RNA database project. Nucl Acids Res 19: 2017–2021
Pedrós-Alió C (1993) Diversity of bacterioplankton. TREE 8: 86–90
Schmidt TM, DeLong EF, Pace NR (1991) Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing. J Bacteriol 173: 4371–4378
Stackebrandt E, Liesack W, Goebel BM (1993) Bacterial diversity in a soil sample from a subtropical Australian environment as determined by 16S rDNA analysis. FASEB J 7: 232–236
Swofford DL (1991) PAUP: Phylogenetic Analysis Using Parsimony version 3.1, Computer program distibuted by the Illinois Natural History Survey Champaign, Illinois
Torsvik V, Goksøyr J, Daae FL (1990) High Diversity in DNA of Soil Bacteria. Appl Environ Microbiol 56: 782–787
Ward DM (1989) Molecular probes for analysis of microbial communities. In: Characklis WG, Wilderer PA (eds) Structure and function of biofilms. John Wiley & Sons Ltd, pp 145–163
Ward DM, Weiler R, Bateson MM (1990) 16S rRNA sequences reveal numerous uncultivated microorganisms in a natural environment. Nature 345: 63–65
Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandier O, Krichevsky MI, Moore C Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) Report of the ad hoc committee on reconciliation of approches to bacterial systematics. Int J Syst Bacteriol 37: 463–464
Weiler R, Weiler JW, Ward DM (1991) 16S rRNA Sequences of uncultivated Hot Spring cyanobacterial mat inhabitants retrieved as randomly primed cDNA. Appl Environ Microbiol 57: 1146–1151
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© 1994 Springer-Verlag Berlin Heidelberg
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Muyzer, G., de Waal, E.C. (1994). Determination of the genetic diversity of microbial communities using DGGE analysis of PCR-amplified 16S rDNA. In: Stal, L.J., Caumette, P. (eds) Microbial Mats. NATO ASI Series, vol 35. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78991-5_21
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DOI: https://doi.org/10.1007/978-3-642-78991-5_21
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