Skip to main content
SpringerLink
Log in

Somatic Embryogenesis and Artificial Seed in Carrot (Daucus carota L.)

  • Chapter
Somatic Embryogenesis and Synthetic Seed II

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 31))

Abstract

Carrot (Daucus carota L.) belongs to the Umbelliferae which is composed of over 350 genera and 3225 species, including many medicinal plants. This family is distributed in Eurasia and Africa, with many horticultural varieties, the roots of which are widely used as food throughout the world. The phenomenon of somatic embryogenesis has been extensively investigated using carrot and other members of the family Umbelliferae.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ammirato PV (1983) The regulation of somatic embryo development in plant cell cultures: suspension culture techniques and hormone requirements. Bio/Technology 1: 68–74

    Article  Google Scholar 

  • De Vries SC, Booij H, Meyerink P, Huiman G, Wilde HD, Thomas TL, van Kämmen A (1988) Acquisition of embryogenic potential in carrot cell-suspension cultures. Planta 176: 196–204

    Article  Google Scholar 

  • Fienberg AA, Choi JH, Lubich LP, Sung ZR (1984) Developmental regulation of polymine metabolism in growth and differentiation of carrot culture. Plant a 162: 532–539

    Article  CAS  Google Scholar 

  • Fujimura T, Komamine A (1975) Effects of various growth regulators on the embryogenesis in a carrot cell suspension culture. Plant Sci Lett 5: 359–364

    Article  CAS  Google Scholar 

  • Fujimura T, Komamine A (1979) Synchronization of somatic embryogenesis in a carrot cell suspension culture. Plant Physiol 64: 162–164

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968). Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Giuliano G, Rosellini D, Terzi M (1983) A new method for purification of the different stages of carrot embryoids. Plant Cell Rep 2: 216–218

    Article  Google Scholar 

  • Grambow HJ, Kao KN, Miller RA, Gamborg OL (1972) Cell division and plant development from protoplasts of carrot cell suspension cultures. Planta 103: 348–355

    Article  Google Scholar 

  • Halperin W (1966) Alternative morphogenetic events in cell suspension. Am J Bot 53: 443–453

    Article  Google Scholar 

  • Hari V (1980) Effects of a cell density change and conditioned media on carrot cell embryogenesis. Z Pflanzenphysiol 96: 227–231

    Google Scholar 

  • Kamada H, Harada H (1979a) Studies on the organogenesis in carrot tissue cultures. I. Effects of growth regulators on somatic embryogenesis and root formation. Z Pflanzenphysiol 91: 255–266

    CAS  Google Scholar 

  • Kamada H, Harada H (1979b) Studies on the organogenesis in carrot tissue cultures. II. Effects of amino acids and inorganic nitrogenous compounds on somatic embryogenesis. Z Pflanzenphysiol 91: 453–463

    CAS  Google Scholar 

  • Kamada H, Kobayashi K, Kiyosue T, Harada H (1989) Stress induced somatic embryogenesis in carrot and its application to synthetic seed production. In Vitro Cell Dev Biol 25: 1163–1166

    Article  Google Scholar 

  • Khan AJ, Minocha SC (1991) Polyamines and somatic embryogenesis in carrot. II. The effect of cyclohexylammonium phosphate. J Plant Physiol 137: 446–452

    CAS  Google Scholar 

  • Kitto SL, Janick J (1985) Hardening treatments increase survival of synthetically-coated asexual embryos of carrot. J Am Soc Hortic Sci 110: 285–286

    Google Scholar 

  • Lin M, Staba J (1961) Peppermint and spearmint tissue cultures. I. Callus formation and submerged culture. Lloydia 24: 139–145

    CAS  Google Scholar 

  • Metting B, Pyne J (1986) Biologically active compounds from microalgae. Enzyme Microb Technol 8: 386–394

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Nomura K, Komamine A (1985) Identification and isolation of single cells that produce somatic embryos at high frequency in a carrot suspension culture. Plant Physiol 79: 988–991

    Article  PubMed  CAS  Google Scholar 

  • Nomura K, Fukuei K, Nitta T (1983) Culture of protoplasts isolated from somatic embryos of carrot. Plant Sci Lett 29: 1–7

    Article  Google Scholar 

  • Nuti-Ronchi V, Caligo MA, Nozzolini M, Luccarini G (1984) Stimulation of carrot embryogenesis by proline and serine. Plant Cell Rep 3: 210–214

    Article  Google Scholar 

  • Redenbaugh K, Fujii J, Slade D, Viss P, Kossler M (1991) Artificial seeds - encapsulated somatic embryos. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 17. High-tech and micropropagation I. Springer, Berlin Heidelberg New York, pp 395–416

    Google Scholar 

  • Reinert J (1958) Untersuchungen über die Morphogenese an Gewebekulturen. Ber Dtsch Bot Ges 71: 15

    Google Scholar 

  • Robie CA, Minocha Sc (1989) Polyamines and somatic embryogenesis in carrot. I. The effects of difluoromethylornithine and difluoromethylarginine. Plant Sci 65: 45–54

    Article  CAS  Google Scholar 

  • Roustan JP, Latche A, Fallot J (1989) Stimulation of Daucus carotasomatic embryogenesis by inhibitors of ethylene synthesis: cobalt and nickel. Plant Cell Rep 8: 182–185

    Article  CAS  Google Scholar 

  • Smith DL, Krikorian AD (1988) Production of somatic embryos from carrot tissues in hormone-free medium. Plant Sci 58: 103–110

    Article  Google Scholar 

  • Smith RH, Kerns HA, Anthony JL, Wild JR (1987) Methotrexate and aminopterin effects on growth and regenearation in Daucus carota.Plant Cell Rep 6: 60–62

    Article  CAS  Google Scholar 

  • Steward FC, Mapes MO, Mears K (1958) Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Am J Bot 45: 705–708

    Article  Google Scholar 

  • Stuart DA, Redenbaugh K (1987) Use of somatic embryogenesis for the regeneration of plants. In: LeBaron HM, Mumma RO, Honeycutt RC, Duesing JH (eds) Biotechnology in agricultural chemistry. Am Chem Soc, Washington, DC. Am Chem Soc Symp Ser 334: 87–96

    Chapter  Google Scholar 

  • Stuart DA, Nelsen J, Strickland SG (1985) Factors affecting developmental processes in alfalfa cell cultures. In: Henke RR, Hughes KW, Costantin MP (eds) Tissue culture in forestry and agriculture. Plenum Press, New York, pp 59–73

    Google Scholar 

  • Sung ZR, Feinberg A, Chorneau R, Borkird C, Turner I, Smith J, Terzi M, LoSchiavo F, Giuliano G, Pitto L, Nuti-Ronchi V (1984) Developmental biology of embryogenesis from carrot cultures. Plant Mol Biol Rep 2: 3–14

    Article  Google Scholar 

  • Wake H, Umetsu H, Ozeki Y, Shimomura K, Matsunaga T (1991) Extracts of marine cyanobacteria stimulated somatic embryogenesis of Daucus carotaL. Plant Cell Rep 9: 655–658

    Article  Google Scholar 

  • Wake H, Akasaka A, Umetsu H, Ozeki Y, Shimomura K, Matsunaga T (1992a) Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium. Plant Cell Rep 11: 62–65

    Article  Google Scholar 

  • Wake H, Akasaka A, Umetsu H, Ozeki Y, Shimomura K, Matsunaga T (1992b) Enhanced germination of artificial seeds by marine cyanobacterial extract. Appl Microbiol Biotechnol 36: 684–688

    Article  Google Scholar 

  • Wethereil DF (1969) Phytochrome in cultured wild carrot tissue. I. Synthesis. Plant Physiol 44: 1734–1737

    Article  Google Scholar 

  • Wethereil DF, Dougall DK (1976) Sources of nitrogen supporting growth and embryogenesis in cultured wild carrot tissue. Physiol Plant 37: 97–103

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biotechnology Section, Central Research Laboratory, Pentel Co. Ltd., Saitama 340, Soka, Japan

    H. Wake

  2. Department of Bioscience and Biotechnology, Aomori Univeristy, Aomori 030, Kobata, Japan

    H. Umetsu

  3. Department of Biotechnology, Tokyo University of Agriculture and Technology, Tokyo 184, Koganei, Japan

    T. Matsunaga

Authors
  1. H. Wake
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Umetsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Matsunaga
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. A-137 New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Wake, H., Umetsu, H., Matsunaga, T. (1995). Somatic Embryogenesis and Artificial Seed in Carrot (Daucus carota L.). In: Bajaj, Y.P.S. (eds) Somatic Embryogenesis and Synthetic Seed II. Biotechnology in Agriculture and Forestry, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78643-3_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-78643-3_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78645-7

  • Online ISBN: 978-3-642-78643-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation