Abstract
The long-term effects of an enriched CO2 atmosphere on the primary carbon metabolism of four year old spruce trees (Picea abies L. Karst) were examined. Six key enzymes were studied in one year old needles of trees exposed for two years in open-top chambers to three CO2 levels (350, 480 and 570 ppmv). The specific activity and quantity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO, EC 4.1.1.39) and the specific activities of the photorespiratory enzymes, glycolate oxidase (EC 1.1.3.15) and hydroxypyruvate reductase (HPR, EC 1.1.1.29) showed significant decreases in the CO2-enriched atmospheres. In contrast, a net increase was found for the specific activities of the mitochondrial enzymes, NAD-malic enzyme (NAD-ME, EC 1.1.1.39) and especially fumarase (EC 4.2.1.2). The carboxylating enzyme, phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31), showed a marked decrease in activity. These results clearly demonstrate increases in the activities of enzymes linked to the respiratory process and decreases in CO2-fixing enzymes as a result of long-term exposure to less than double the present ambient level of CO2.
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van Oosten, JJ., Dizengremel, P., Laitat, E., Impens, R. (1993). Too Much of a Good Thing? Long-Term Exposure to Elevated CO2 Decreases Carboxylating and Photorespiratory Enzymes and Increases Respiratory Enzyme Activity in Spruce. In: Jackson, M.B., Black, C.R. (eds) Interacting Stresses on Plants in a Changing Climate. NATO ASI Series, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78533-7_11
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DOI: https://doi.org/10.1007/978-3-642-78533-7_11
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