Abstract
DNA purified from more than 170 forensic samples was characterized by restriction fragment length polymorphism analysis. DNA isolated from dried blood stains, various tissues, and semen stains was digested with the restriction endonuclease Pst 1, size separated by agarose gel electrophoresis, transferred to nylon membrane and hybridized with IM probes which recognize hypervariable regions in the human genome. The size of DNA fragments, reacting to these probes, was measured with a computer assisted digitizing system and a likelihood of identity calculated from databases containing the frequency of the alleles identified with each probe. The combined discrimination potential with 4 DNA probes that hybridize to hypervariable regions is greater than 108. The present report also summarizes some of our experience in the analysis of forensic biological evidence.
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References
Botstein, D., White, R., Skolnick, M. and Davis, R., “Construction of a Genetic Linkage Map in Man Using Restriction Fragment Length Polymorphisms”, American Journal of Human Genetics, vol. 32, 1980, pp. 314–331.
Kanter, E., Baird, M., Shaler, R., Balazs, I., “Analysis of Restriction Length Polymorphisms in Deoxyribonucleic Acid (DNA) Recovered from Dried Bloodstains”, Journal of Forensic Sciences, vol. 31, 1986, pp. 403–408.
Giusti, A., Baird, M., Pasquale, S., Balazs, I., and Glassberg, J., “Application of Deoxyribonucleic Acid (DNA) Polymorphisms to the Analysis of DNA Recovered from Sperm”, Journal of Forensic Sciences, vol. 31, 1986, pp. 409–417.
Baird, M., Balazs, Giusti, A., Miyazaki, L., Nicholas, L., Wexler, K., Kanter, E., Glassberg, J., Allen, F., Rubinstein, P., and Sussman, L., “Allele Frequency Distribution of Two Highly Polymorphic DNA Sequences in Three Ethnic Groups and Its Application to the Determination of Paternity,” American Journal of Human Genetics, vol. 39, 1986, pp. 489–501.
Cooke, H., Brown, W. and Rappold, G., “Hypervariable Telomeric Sequences From The Human Sex Chromosomes Are Pseudoautosomal”, Nature, vol. 317, 1985, pp. 687–692.
Feinberg, A. and Vogelstein, B., “A Technique For Radiolabeling DNA Restriction Endonuclease Fragments To High Specific Activity”, Analytical Biochemistry, vol. 137, 1983, pp. 6–13.
Fisher, R., “Standard Calculations for Evaluating a Blood-Group System”, Heredity, vol. 5, 1960, pp. 95–102.
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© 1988 Springer-Verlag Berlin Heidelberg
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Baird, M. et al. (1988). The Application of DNA-Print for Identification from Forensic Biological Materials. In: Mayr, W.R. (eds) Advances in Forensic Haemogenetics. Advances in Forensic Haemogenetics, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73330-7_77
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DOI: https://doi.org/10.1007/978-3-642-73330-7_77
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-18765-3
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