Changes in Important Biochemical Parameters Due to Extreme Haemodilution with Cross-linked Stromafree Haemoglobin Solution

Abstract

Transport of biological available oxygen in vertebrates is performed by two different principles: physical solution in plasma and chemical binding to the haemoglobin molecule. According to these principles the development of oxygen-carrying colloids proceeds along two different pathways. The fluorocarbons increase the amount of physically dissolved oxygen due to the extraordinary oxygen-solubility of these compounds [7]. Some research groups have favoured the development of stroma-free haemoglobin solutions (SFH). These compounds chemically bind oxygen similar to the natural intraerythrocytic haemoglobin [2, 6]. In particular, Bonhard and coworkers have been able to improve the oxygen-transporting property and the intravascular persistence of these SFH solutions [1, 3]. The former was achieved by pyridoxalation and the latter by intermolecular crosslinkage of haemoglobin molecules. This new type of SFH was called “nhpa”, where “n” means polymer, “h” means haemoglobin, “p” means pyridoxalation and “a” means albumin addition for correction of oncotic pressure. This 8.5% nhpa is a research product of Biotest-Pharma, Frankfurt. With this oxygen-carrying colloid we performed extreme isovolemic haemodilution in dogs and examined the changes in different biochemical parameters.