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Specific Volumes of Biological Macromolecules and Some Other Molecules of Biological Interest

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Thermodynamic Data for Biochemistry and Biotechnology

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Abbreviations

B:

binding of a component to a macromolecule (g g- 1)

E:

electrostatic (Donnan) exclusion of a component from a macromolecule (g g-1)

M:

molecular weight

N:

number of components

P:

pressure

T:

temperature (K)

V:

total volume (cm3)

V:

partial molar volume of a component (cm3 mol- 1)

W:

weight percentage of a component

c:

concentration of a component (g cm- 3)

f:

weight fraction of a component

g:

grams of a component

h:

hydration (mol mol- 1)

i:

any solution component

j:

any nonmacromolecular solution component

k:

apparatus constant of digital density meter

m:

molality

n:

number of moles

\(\rm \overline v \) :

partial specific volume of a component (em3 g- 1); cf. Eq. (8)

\(\rm \overline v \):a :

assumed (average) value for the partial specific volume (cm3 g- 1)

\(\rm \overline v \) c :

calculated partial specific volume (cm3 g- 1)

\(\rm \overline v \):c)mod.:

calculated partial specific volume (cm3 g-1), obtained from modified procedures

v′:

isopotential specific volume (cm3 g- 1); cf. Eq. (11)

w:

weight molality (g g-1)

δ:

amount of ligand (e.g., detergent) bound to a macromolecule (g g-1)

μ:

chemical potential

ξ:

preferential interaction parameter (g g- 1)

ρ:

density (g cm- 3), density of the solution (g cm- 3)

τ:

period of oscillation

Φ:

apparent specific volume (cm3 g- 1); cf. Eq. (9)

Φ′:

apparent isopotential specific volume (cm3 g-1); cf. Eq. (12)

Φ′c :

calculated isopotential specific volume (cm3 g- 1)

1:

component 1, principal solvent (generally water)

2:

component 2, macromolecule (e.g., a protein)

3:

component 3, a membrane-diffusible solute in the solvent medium (e.g., a salt)

d:

detergent

l:

ligand

np:

nonprotein component

p:

protein component

r:

amino acid residue

s:

complex solvent mixture (e.g., water+salt(s), buffer)

0:

vanishing concentration of the ith component

′:

designates isopotential conditions

BSA:

bovine serum albumin

DTE:

dithioerythritol

DTT:

dithiothreitol

GuHCl:

guanidine hydrochloride

2-ME:

2-mercaptoethanol

SDS:

sodium dodecyl sulfate

cmc:

critical micellar concentration

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Durchschlag, H. (1986). Specific Volumes of Biological Macromolecules and Some Other Molecules of Biological Interest. In: Hinz, HJ. (eds) Thermodynamic Data for Biochemistry and Biotechnology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71114-5_3

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  • DOI: https://doi.org/10.1007/978-3-642-71114-5_3

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71116-9

  • Online ISBN: 978-3-642-71114-5

  • eBook Packages: Springer Book Archive

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