Summary
Indol-3-acetic acid (IAA) was analysed in the culture medium of Azospirillum brasilense using a fluorimetric detector coupled to a reversed phase high performance liquid chromatography column. Accuracy of the analysis was confirmed by successive approximation and additionally verified by the 2-methyl indolo-α-pyrone assay. When A. brasilense was grown aerobically and in the presence of ammonium up to 40 ng.ml−1 IAA was excreted into the medium in the absence of any added tryptophane. IAA concentrations in the medium increased mainly during the early stationary phase and remained remarkably constant afterwards.
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References
Baldani, V.L.D., Baldani, J.I. and Dôbereiner, J. (1983). Can. J. Microbiol. 29, 924–929
Millet, E., Avivi, Y. and Feldman, M. (1984). Plant and Soil 80, 261–266
O’Hara, G.W., Davey, M.R. and Lucas, J.A. (1981). Can. J. Microbiol. 21, 871–877
Okon, Y., Heytier, P.G. and Hardy, R.F.W. (1983). Applied and Env. Microbiol. 46 (3), 694–697
Reynders, L. and Vlassak, K. (1982). In “Azospirillum Genetics, Physiology, Ecology” Experientia Suppl., Klingmuller (Ed.) 42, 121–129
Tien, T.M., Gaskins, M.H. and Hubbell, D.H. (1979). Applied and Environm. Microbiol. 37 (5), 1016–1024
Inbal, E. and Feldman, M. (1982). Israël J. Botany 31, 257–263
Dubois, M. (1983). Ph.D. Thesis, Institut national agronomique, Paris
Plazinski, J. and Rolfe, B.G. (1985). Applied and Environmental Microbiol. 49 (4), 990–993
Reynders, L. and Vlassak, K. (1979). Soil Biol. Biochem. 11, 547–548
Hartmann, A., Mahavir Singh and Klingmiiller, W. (1983). Canadian J. Microbiol. 29 (8), 916–923
Pegg, G.F. (1976). In “Physiological Plant Pathology”, Hertefuss and Williams (Eds.) 560–591
Crozier, A. and Reeve, D.R. (1980). In “Hormonal regulation of development I”, McMillan (Ed.), Enc. of Plant Physiology New Series 9, 203–280
Trewavas, A. (1981). Plant, Cell and Environment 203–228
Raven, J.A. (1975). New Phytol. 74, 163–172
Rinaudo, G. (1983). Ph.D. Thesis, Université de Paris-Sud, Centre d’Orsay
Döbereiner, R.J. and Day, J.M. (1976). In A“Proceedings of the 1st Int. Symp. on Nitrogen Fixation”Newton, E. and Nijman, C.J. (Eds.) 2, 518–538
Horemans, S., Van Onckelen, H.A., Rudelsheim, P. and De Greef, J.A. (1984). J. Exp. Bot., 35, 1832–1845
Hartmann, A., Fusseder, A. and Klingmuller, W. (1983). In Klingmuller, W. (ed.): Azospirillum II, Genetics Physiology Ecology. Experientia Suppl. 48, 78–88
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© 1985 Springer-Verlag Berlin Heidelberg
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Horemans, S., Vlassak, K. (1985). Production of Indol-3-Acetic Acid by Azospirillum Brasilense. In: Klingmüller, W. (eds) Azospirillum III. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70791-9_10
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DOI: https://doi.org/10.1007/978-3-642-70791-9_10
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