Abstract
Colony-stimulating factors (CSFs) are a family of hematopoietic growth factors required for the proliferation and differentiation of hematopoietic progenitor cells [1, 2]. In the human system, purification to homogeneity and biochemical characterization has only been reported for macrophage- active CSF (CSF-1) [3]. However, there are many reports about highly purified human granulocyte-macrophage CSFs (e. g. [4–7]), but not about pluripotent human CSF.
Laboratory of Developmental Hematopoiesis and Laboratory of Molecular Hematology,, Memorial Sloan-Kettering Cancer Center, New York, NY 10021 Supported by NIH grants CA 20194, CA 31780, CA 32516, CA 33873, and CA 34995, American Cancer Society grants CH 251 and CH-3GH, NCI grant K08-CA00966-01, and the Gar Reichman Foundation
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References
Burgess AW, Metealf D (1980) The nature and action of granulocyte-macrophage colony-stimulating factors. Blood 56: 947–958
Nicola NA, Vadas M (1984) Hematopoietic colony-stimulating factors. Immunol Today 5: 76–81
Das SK, Stanley ER (1982) Structure-function studies of a colony stimulating factor (CSF-1). J Biol Chem 257:13 679–13 684
Wu MC, Yunis AA (1980) Common pattern of two distinet types of colony-stimulating factor in human tissues and cultured cells. J Cün Invest 65: 772–775
Lusis AJ, Quon DH, Golde DW (1981) Purification and characterization of a human T-lymphocyte-derived granulocyte-macrophage colony-stimulating factor. Blood 57: 13–21
Abboud CN, Brennan JK, Barlow GH, Lichtman MA (1981) Hydrophobic adsorption chromatography of colony-stimulating activities and erythroid-enhancing activity from the human monocyte-like cell line CGT. Blood 58: 1148–1154
Motoyoshi K, Suda T, Kusumoto K, Takaku F, Miura Y (1982) Granulocyte-macrophage colony-stimulating and binding activities of purified human urinary colony-stimulating factor to murine and human bone marrow cells. Blood 60: 1378–1391
Fauser AA, Messner HA (1978) Granulocyte- erythropoietic colonies in human bone marrow, peripheral blood and cord blood. Blood 52: 1243–1248
Fauser AA, Messner HA (1979) Identification of megakaryocytes, macrophages and eosinophils in colonies of human bone marrow containing neutrophilic granulocytes and erythroblasts. Blood 53: 1023–1027
Messner HA, Izaguirre CA, Jamal N (1981) Identification of T lymphocytes in human mixed hematopoietic colonies. Blood 58: 402–405
Ruppert S, Lohr GW, Fauser AA (1983) Characterization of stimulatory activity for human pluripotent stem cells ( CFUGEMM ). Exp Hematol 11: 154–161
Myers CD, Katz FE, Joshi G, Miliar JL (1984) A cell line secreting stimulating factors for CFU-GEMM culture. Blood 64: 152–155
Salahuddin SZ, Markham PD, Lindner SG, Gootenberg J, Popovic M, Hemmi H, Sarin PS, Gallo RC (1984) Lymphokine production by cultured human T cells transformed by human T cell leukemia-lymphoma virus-I. Science 223: 703–707
Broxmeyer HE, Moore MAS, Ralph P (1977) Cell-free granulocyte colony inhibitory activity derived from human polymorphonuclear neutrophils. Exp Hematol 5: 87–102
Li L, Broxmeyer HE, Meyers PA, Moore MAS, Tzvi Thaler H (1983) Association of cell cycle expression of Ia-like antigenic determinants on normal human multipotential (CFU-GEMM) and erythroid ( BFU-E) progenitor cells with regulation in vitro by acidic isoferritins. Blood 61: 250–256
Metealf D (1980) Clonal extinetion of myelomonocytic leukemic cells by serum from mice injected with endotoxin. Int J Cancer 25: 225–233
Svet-Modalsky GJ, Zinzer SN, Svet-Modalsky IA, Mann PE, Holland JF, Fogh J, Arlin Z, Clarkson BD (1980) CSF-producing human tumor cell lines. Lack of CSF activity of human stromal bone marrow fibroblasts. Exp Hematol 8 [Suppl] 7: 76 (abstract)
Welte K, Wang CY, Mertelsmann R, Venuta S, Feldman S, Moore MAS (1982) Purification of human interleukin 2 to apparent homogeneity and its molecular heterogeneity. J Exp Med 165: 454–464
Ralph P, Welte K, Levi E, Nakoinz I, Litkovsky PB, Mertelsmann R, Moore MAS (1984) Human B cell-inducing factor(s) for production of IgM, IgG and IgA: independence from IL2. J Immunol 132: 1858–1862
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Ihle JN, Keller J, Henderson L, Klein F, Palaszynski E (1982) Procedures for the purification of interleukin 3 to homogeneity. J Immunol 129: 2431–2436
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Welte, K. et al. (1985). Purification to Apparent Homogeneity and Biochemical Characterization of Human Pluripotent Hematopoietic Colony-Stimulating Factor. In: Neth, R., Gallo, R.C., Greaves, M.F., Janka, G. (eds) Modern Trends in Human Leukemia VI New Results in Clinical and Biological Research Including Pediatric Oncology. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 29. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70385-0_82
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DOI: https://doi.org/10.1007/978-3-642-70385-0_82
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