Abstract
The haemolytic plaque assay introduced by Jerne and Nordin (1963) has been the most widely used test system for enumeration of specific antibody-secreting cells (ASC). This system was originally developed to detect cells secreting complement binding antibodies against erythrocytes which eventually resulted in lysis of the red target cells. When this assay was applied to soluble antigens several inherent limitations became obvious, such as instability of the conjugated erythrocytes, inconsistent lysability of different batches of antigen-coated red cells, uncertainty about the antibody isotype involved in formation of direct plaque, and effort and time required by this assay procedure, making it unsuited for a large number of samples.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Borrebaeck CAK (1983a) In vitro immunization for the production of antigen-specific lymphocyte hybridomas. Scand J Immunol 18:9–12
Borrebaeck CAK (1983b) In vitro immunization of mouse spleen cells and the production of monoclonal antibodies. Acta Chem Scand B37 7:647–648
Borrebaeck CAK (1984) Dependence on T cell replacing factor and immunogenic dose for the production of monoclonal antibodies using the in vitro immunization technique. Mol Immunol 21:841–845
Borrebaeck CAK, Etzler ME (1981) Production and characterization of a monoclonal antibody against the seed lectin of the Dolichos biflorus plant. J Biol Chem 256:4723–4725
Czerkinsky CC, Nilsson LÅ, Nygren H, Ouchterlony Ö, Tarkowski A (1983) A solid-phase enzyme linked immunospot (ELISPOT) assay for enumeration of antibody-secreting cells. J Immunol Methods 65:109–121
Jerne NK, Nordin AA (1963) Plaque formation in agar by single antibody producing cells. Science 140:405
Sedgwick JD, Holt PO (1983) A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J Immunol Methods 57:301–309
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Möller, S.A., Borrebaeck, C.A.K. (1985). A Microfilter Assay for the Detection of Antibody-Producing Cells In Vitro. In: Habermehl, KO. (eds) Rapid Methods and Automation in Microbiology and Immunology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69943-6_16
Download citation
DOI: https://doi.org/10.1007/978-3-642-69943-6_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-69945-0
Online ISBN: 978-3-642-69943-6
eBook Packages: Springer Book Archive