Abstract
The covalent binding of [3H]aflatoxin B1 (AF) to liver DNA was determined, 6h after oral administration to male rabbits. A Covalent Binding Index, CBI (μmol AF/mol DNA-P)/(mmol AF/kg b. w.) = 8,500 was found. Pretreatment of rabbits with AF coupled to bovine serum albumin in Freund’s adjuvant led to the production of AF-directed antibodies. Administration of [3H]AF to such immunized rabbits resulted in a CBI of only 2,500, i.e., the immunization provided a protection by a factor of more than 3. Although this is encouraging evidence for the potential of active immunization against genotoxic carcinogens, a number of points will have to be clarified, such as the time course for the DNA binding and the question of a possible shift to other target cells.
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© 1984 Springer-Verlag
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Caviezel, M., Aeschbach, A.P., Lutz, W.K., Schlatter, C. (1984). Reduction of Covalent Binding of Aflatoxin B1 to Rabbit Liver DNA After Immunization Against this Carcinogen. In: Chambers, P.L., Preziosi, P., Chambers, C.M. (eds) Disease, Metabolism and Reproduction in the Toxic Response to Drugs and Other Chemicals. Archives of Toxicology, vol 7. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69132-4_36
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DOI: https://doi.org/10.1007/978-3-642-69132-4_36
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