Abstract
Corynebacterium fascians causes fasciation disease or witches’ broom in dicotyledonous plants. The disease is characterized by release of apical dominance and outgrowth of lateral buds, thus giving rise to the characteristic witches’ broom syndrome (Roussaux, 1965). The symptoms of the disease can be duplicated by treatment of seedlings with cytokinins, indicating that the disease may be caused by cytokinins produced by the microbe (Thimann and Sachs, 1966). This work deals with the kinds and quantities of cytokinins released into culture media and present in tRNA of five strains of C. fascians, ranging from highly virulent MW2, moderately virulent Cf2 and Cfl, weakly virulent Cf 15 to avirulent Cfl6. It examines the relationships of the presence of plasmids to cytokinin production and to pathogenicity. It also reports the isolation from highly virulent MW2 of activity of cytokinin synthase that catalyzes the formation of N6-(Δ2-isopentenyl) adenosine-5′-monophosphate (i6 AMP) from 5′-AMP and Δ2-IPP.
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References
Armstrong DJ, Scarbrough E, Skoog F, Cole DL, Leonard NJ (1976) Cytokinins in Corynebacterium fascians cultures. Isolation and identification of 6-(4-hydroxy-3-methyl-as-2-butenylamino)-2-methylthiopurine. Plant Physiol 58: 749–752
Banthorpe DV, Doonan S, Gwtowski J A (1977) Isopentenyl pyrophosphate isomerase from pig liver. Arch Biochem Biophys 184: 381–390
Chapman NW, Morris RO, Zaerr JB (1976) Occurence of iraws-ribosylzeatin in Agrobacterium tumefaciens tRNA. Nature (London) 262: 153–154
Chen CM, Melitz DK (1979) Cytokinin biosynthesis in a cell-free system from cytokinin-auto-trophic tobacco tissue cultures. FEBS Lett 107: 15–20
Doyle ME, Hanson RH (1980) Unpublished observation
Engelbrecht L (1971) Cytokinin activity in larval infected leaves. Biochem Physiol Pflanz 162: 9–27
Greene EM (1980) Cytokinin production by microorganisms. Bot Rev 46: 25–74
Helgeson JP, Leonard NJ (1966) Cytokinins: identification of compounds isolated from Corynebacterium fascians. Proc Natl Acad Sci USA 56: 60–63
Hurlbert RB, Schmitz H, Brumm AF, Potter VR (1954) Nucleotide metabolism II. Chromatographic separation of acid-soluble nucleotides. J Biol Chem 209: 23–39
Kaiss-Chapman RW, Morris RO (1977) Trans-zzaim in culture filtrates of Agrobacterium tumefacions. Biochem Biophys Res Commun 76: 453–459
Klämbt D, Thies G, Skoog F (1966) Isolation of cytokinins from Corynebacterium fascians. Proc Natl Acad Sci USA 56: 52–59
Kline LK, Fittler FA, Hall RG (1969) N6-(Δ2-Isopentenyl)adenosine. Biosynthesis in transfer ribonucleic acid in vitro. Biochemistry 8: 4361–4371
Miller CO (1967) Zeatin and zeatin riboside from a mycorrhizal fungus. Science 157: 1055–1057
Murai N, Skoog F, Doyle ME, Hanson RS (1979) Correlation between cytokinin production, presence of plasmids, and pathogenicity in strains ofCorynebacterium fascians. 10th Int Conf Plant Growth Subst, Madison, WI, p 268
Murai N, Skoog F, Doyle ME, Hanson RS (1980) Relationship between cytokinin production, presence of plasmids and fasciation-caused by strains of Corynebacterium fascians. Proc Natl Acad Sci USA 77: 619–623
Roussaux M J (1965) Etude préliminaire des modifications induites chez le pois express Alaska par le Corynebacterium fascians (Tilford) Dowson. Rev Gen Bot 72: 21–53
Scarbrough E, Armstrong DJ, Skoog F, Frihart CR, Leonard NJ (1973) Isolation of czs-zeatin from Corynebacterium fascians. Proc Natl Acad Sci USA 70: 3825–3829
Skoog F, Schmitz RY (1979) Biochemistry and Physiology of Cytokinins. In: Litwack G (ed) Biochemical actions of hormones, vol VI. Academic Press, London, New York, pp 335–413
Taya Y, Tanaka Y, Nishimura S (1978) 5′-AMP is a direct precursor of cytokinin in Dictyostelium discoideum. Nature (London) 271: 545–547
Tchen TT (1963) Preparation and determination of intermediates in cholesterol synthesis. In: Colowick SP, Kaplan NO (ed) Method Enzymol, vol VI, Academic Press, London, New York, pp 505–512
Thimann KV, Sachs T (1966) The role of cytokinins in the “fasciation” disease caused by Corynebacterium fascians. Am J Bot 53: 731–739
Van Studen J (1975) Cytokinins from larvae in Erythrina latissima galls. Plant Sci Lett 5: 227–230
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© 1981 Springer-Verlag, Berlin Heidelberg
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Murai, N. (1981). Cytokinin Biosynthesis and Its Relationship to the Presence of Plasmids in Strains of Corynebacterium fascians . In: Guern, J., Péaud-Lenoël, C. (eds) Metabolism and Molecular Activities of Cytokinins. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68035-9_2
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DOI: https://doi.org/10.1007/978-3-642-68035-9_2
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