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The Current Status of Cytokinin-Binding Moieties

  • Conference paper
Metabolism and Molecular Activities of Cytokinins

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Several years ago we described (Fox and Erion, 1975, 1977) the properties of a soluble protein (CBF-1) isolated from wheat germ which binds eytokinins with relatively high affinity and specificity. This study was stimulated by an earlier report from investigators in Letham’s laboratory (Berridge et al., 1970) who described nonsaturable, multisite, reversible binding of kinetin to ribosomes isolated from Brassica pekinensis leaves. Although Scatchard plots of binding were not presented and the investigators were unable to solubilize a binding substance, their data indicate a low affinity, nonspecific binding of the kind seen in wheat germ ribosomes (Fox and Erion, 1977) at the high cytokinin concentrations used in their study. However a wheat embryo protein having a relatively high affinity for eytokinins has recently been studied in two other laboratories (Polya and Davis, 1978; Polya and Bowman, 1979; Moore, 1979) and is apparently identical with CBF-1.

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Abbreviations

CBF-1:

wheat germ cytokinin binding protein

bzl6 Ade:

6-benzylaminopurine

i6 Ade:

6(Δ2-isopentenylamino) purine

io6 Ade:

Zeatin

OH-PA:

6 (5’-hydroxy-n-pentylamino) purine

i6 Ado:

N6 (Δ2-isopentenyl)adenosine

io6 Ado:

Zeatin Riboside

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Author information

Authors and Affiliations

  1. Departments of Biochemistry and Botany, University of Kansas, Lawrence, KS, 66045, USA

    J. E. Fox

Authors
  1. P. Keim
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  2. J. Erion
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  3. J. E. Fox
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Editor information

Editors and Affiliations

  1. Laboratoire de Génétique et Physiologie du Développement des Plantes, CNRS, F-91190, Gif-sur-Yvette, France

    Jean Guern

  2. Faculté des Sciences de Luminy, Case 901 Laboratoire de Biochimie, Fonctionnelle des Plantes, ER 104 CNRS, F-13288, Marseille Cedex 9, France

    Claude Péaud-Lenoël

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© 1981 Springer-Verlag, Berlin Heidelberg

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Keim, P., Erion, J., Fox, J.E. (1981). The Current Status of Cytokinin-Binding Moieties. In: Guern, J., Péaud-Lenoël, C. (eds) Metabolism and Molecular Activities of Cytokinins. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68035-9_17

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  • DOI: https://doi.org/10.1007/978-3-642-68035-9_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-68037-3

  • Online ISBN: 978-3-642-68035-9

  • eBook Packages: Springer Book Archive

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