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Anther Culture

  • Chapter
Experimental Embryology of Vascular Plants

Abstract

More than two decades ago, C.D. LaRue (1954) of the University of Michigan, Ann Arbor, USA, conceived the idea of culturing pollen (gametophytes) of higher plants in order to explore their growth potential under in vitro conditions. He carried out extensive investigations with the male and female gametophytes of a number of gymnosperms in aseptic media and showed that these were capable of unusual growth by repeated mitotic divisions of the pollen or the ovular nucellus. The nucellar tissue regenerated roots and shoots. In spite of their sporophytic appearance, these structures were haploid. Similar studies with angiosperms, however, did not succeed.

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References

  • Abo-el-Nil MM, Hildebrandt AC (1971) Differentiation of virus-symptomless geranium plants from anther callus. Plant Dis Rep 55:1017–1020

    Google Scholar 

  • Anagnostakis SL (1974) Haploid plants from anthers of tobacco: enhancement with charcoal. Planta 115:281–283

    Google Scholar 

  • Babbar SB, Mittal A, Gupta SC (1980) In vitro induction of androgenesis, callus formation and organogenesis in Iberis amara Linn, anthers. Z. Pflanzenphysiol 100:409–414

    CAS  Google Scholar 

  • Bajaj YPS (1976) In vitro induction of haploid plants. In: Evans PK (ed) Towards plant improvement by in vitro methods. Academic Press, London New York, in press

    Google Scholar 

  • Bajaj YPS (1978) Regeneration of haploid tobacco plants from isolated pollen grown in drop culture. Indian J Exp Biol 16:407–409

    Google Scholar 

  • Bajaj YPS (1981) Regeneration of plants from ultra-low frozen anthers of Primula obconica. Scientia Hort 14:93–95

    Google Scholar 

  • Bajaj YPS, Pierik RLM (1974) Vegetative propagation of Freesia through callus cultures. Neth J Agric Sci 22:153–159

    Google Scholar 

  • Bajaj YPS, Reinert J, Heberle E (1977) Factors enhancing in vitro production of haploid plants. In: Gautheret RJ (ed) La culture de tissus et des cellules de végétaux. Masson, Paris, pp 47–58

    Google Scholar 

  • Ban Y, Kokubu T, Miyaji Y (1971) Production of haploid plants by anther culture of Setaria italica. Bull Fac Agric Kagoshima Univ 21:77–81

    Google Scholar 

  • Barclay IR (1975) High frequencies of haploid production in wheat (Triticum aestivum) by chromosome elimination. Nature (London) 256:410–411

    Google Scholar 

  • Bernhard S (1971) Développement d’embryons haploîdes a partir d’anthères cultivées in vitro. Etude cytologique comparée chez le tabac et le Petunia. Rev Cytol Biol Vég 34:165–188

    Google Scholar 

  • Binding H, Binding K, Straub J (1970) Selektion in Gewebekulturen mit haploiden Zellen. Naturwissenschaften 57:138–139

    PubMed  CAS  Google Scholar 

  • Bourgin JP (1978) Valine-resistant plants from in vitro selected tobacco cells. Mol Gen Genet 161:225–230

    CAS  Google Scholar 

  • Bourgin JP, Nitsch JP (1967) Obtention de Nicotiana haploîdes à partir d’étamines cultivées in vitro. Ann Physiol Vég 9:377–382

    Google Scholar 

  • Carlson PS (1970) Induction and isolation of auxotrophic mutants in somatic cell cultures of Nicotiana tabacum. Science 168:487–489

    PubMed  CAS  Google Scholar 

  • Carlson PS (1973) Methionine sulfoximine-resistant mutants of tobacco. Science 180:1366

    PubMed  CAS  Google Scholar 

  • Chandy LP, Narayanaswamy S (1971) Diploid and haploid androgenic plantlets from haploid Datura in vitro. Indian J Exp Biol 9:472–475

    Google Scholar 

  • Chen CH, Chen FT, Chien CF, Wang CH, Chang SJ, Hsu HE, Ou HH, Ho YT, Lu TM (1979) A process of obtaining pollen plants of Hevea brasiliensis Muell-Arg. Sci Sinica 22:81–90

    Google Scholar 

  • Cheng-hua Chen, Fa-tsu Chen, Chang-fa Chien, Chuan-hua Wang (1978) Obtaining pollen plants of Hevea brasiliensis Muell. Arg. In: Proc Symp Plant Tissue Culture, Peking Sci Press, Peking, pp 11–21

    Google Scholar 

  • Clapham D (1971) In vitro development of callus from the pollen of Lolium and Hordeum. Z Pflanzenzucht 65:285–292

    Google Scholar 

  • Clapham D (1973) Haploid Hordeum plants from anthers in vitro. Z Pflanzenzücht 69:142–155

    Google Scholar 

  • Clapham D (1977) Haploid induction in cereals. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 279–340

    Google Scholar 

  • Collins GB, Dunwell JM, Sunderland N (1974a) Irregular microspore formation in Datura innoxia and its relevance to anther culture. Protoplasma 82:365–378

    Google Scholar 

  • Collins GB, Legg PD, Kasperbauer MJ (1974b) Use of anther-derived haploids in Nicotiana. I. Isolation of breeding lines differing in total alkaloid content. Crop Sci 14:77–80

    CAS  Google Scholar 

  • Corduan G (1975) Regeneration of anther-derived plants of Hyoscyamus niger L. Planta 127:27–36

    Google Scholar 

  • Corduan G, Spix C (1975) Haploid callus and regeneration of plants from anthers of Digitalis purpurea L. Planta 124:1–12

    Google Scholar 

  • Corduan G, Spix C (1978) Anther culture of a hybrid of the genus Hyoscyamus, a rapid method to obtain homozygous recombinants. In: Alfermann AW, Reinhard E (eds) Production of natural compounds by cell culture methods. Int Symp Plant Cell Culture Munich, pp 295–302

    Google Scholar 

  • Craig IL (1974) Haploid plants (2n = 21) from in vitro anther culture of Triticum aestivum. Can J Genet Cytol 16:697–700

    Google Scholar 

  • Debergh P, Nitsch C (1973) Premiers résultats sur la culture in vitro de grains de pollen isolés chez la tomate. CR Acad Sci Paris 276:1281–1284

    Google Scholar 

  • De Buyser J, Henry Y (1980) Induction of haploid and diploid plants through in vitro anther culture of haploid wheat (n = 3x = 21). Theor Appl Genet 57:57–58

    Google Scholar 

  • Devreux M (1970) New possibilities for the in vitro cultivation of plant cells. Euro-Spectra 9:105–110

    Google Scholar 

  • Devreux M, de Nettancourt D (1974) Screening mutations in haploid plants. In: Kasha KJ (ed) Haploids in higher plants: Advances and potential. Guelph Univ, Guelph, pp 309–322

    Google Scholar 

  • Devreux M, Saccardo F (1971) Mutazioni sperimentali osservate su piante aploidi di tabacco ottenute per culture in vitro di antere irradiate. Am Ass Genet 16:69–71

    Google Scholar 

  • Dix PJ, Street HE (1975) Sodium chloride resistant cultured cell lines from Nicotiana sylvestris and Capsicum annuum. Plant Sci Lett 5:231–237

    Google Scholar 

  • Doreswamy R, Chacko EK (1973) Induction of plantlets and callus from anthers of Petunia axillaris (Lam.) BSP cultured in vitro. Hort Res 13:41–44

    Google Scholar 

  • Doy CH, Gresshoff PM, Rolfe BG (1972) Transfer and expression of bacterial genes in plant cells. Search 3:447–448

    Google Scholar 

  • Dunwell JM, Sunderland N (1973) Anther culture of Solanum tuberosum L. Euphytica 22:317–323

    Google Scholar 

  • Engvild KC (1973) Triploid petunias from anther cultures. Hereditas 74:144–147

    Google Scholar 

  • Engvild KC, Linde-Laursen I, Lundqvist A (1972) Anther cultures of Datura innoxia: flower bud stage and embryoid level of ploidy. Hereditas 72:331–332

    Google Scholar 

  • Foroughi-Wehr B, Wilson HM, Mix G, Gaul H (1977) Monohaploid plants from anthers of a dihaploid genotype of Solanum tuberosum L. Euphytica 26:361–367

    Google Scholar 

  • Gamborg OL, Constabel F, Miller RA (1970) Embryogenesis and production of albino plants from cell cultures of Bromus inermis. Planta 95:355–358

    Google Scholar 

  • Geier T, Kohlenbach HW (1973) Entwicklung von Embryonen und embryogenem Kallus aus Pollenkörnern von Datura meteloides und Datura innoxia. Protoplasma 78:381–396

    Google Scholar 

  • George L, Narayanaswamy S (1973) Haploid Capsicum through experimental androgenesis. Protoplasma 78:467–470

    Google Scholar 

  • George L, Rao PS (1979) Experimental induction of triploid plants of Physalis through anther culture. Protoplasma 100:13–19

    Google Scholar 

  • Goodsell SF (1961) Male sterility in corn by androgenesis. Crop Sci 1:227–228

    Google Scholar 

  • Gresshoff PM, Doy CH (1972a) Haploid Arabidopsis thaliana callus and plants from anther culture. Aust J Biol Sci 25:259–264

    Google Scholar 

  • Gresshoff PM, Doy CH (1972b) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 107:161–170

    Google Scholar 

  • Grunewaldt LJ, Malepszy S (1975) Observations on anther callus from Hordeum vulgare. Z Pflanzenphysiol 75:55–62

    Google Scholar 

  • Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature (London) 204:497

    Google Scholar 

  • Guha S, Maheshwari SC (1966) Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Nature (London) 212:97–98

    Google Scholar 

  • Guha S, Maheshwari SC (1967) Development of embryoids from pollen grains of Datura in vitro. Phytomorphology 17:454–461

    Google Scholar 

  • Guha S, Iyer RD, Gupta S, Swaminathan MS (1970) Totipotency of gametic cells and the production of haploids in rice. Curr Sci 39:174–176

    Google Scholar 

  • Guha-Mukherjee S (1973) Genotypic differences in the in vitro formation of embryoids from rice pollen. J Exp Bot 24:139–144

    Google Scholar 

  • Harn C (1971) Studies on anther culture in Solanum nigrum. SABRAO Newslett 3:39–42

    Google Scholar 

  • Harn C, Kim MZ (1972) Induction of callus from anthers of Prunus armeniaca. Korean J Breed 4:49–53

    Google Scholar 

  • Hoffmann F (1978) Mutation and selection of haploid cell culture systems of rape and rye. In: Alfermann AW, Reinhard E (eds) Production of natural compounds by cell culture methods. Proc Int Symp Plant Cell Culture, Munich, pp 319–329

    Google Scholar 

  • Horner M, McComb JA, McComb A J, Street HE (1977) Ethylene production and plantlet formation by Nicotiana anthers cultured in the presence and absence of charcoal. J Exp Bot 28:1365–1372

    CAS  Google Scholar 

  • Hughes KW, Bell SL, Caponetti JD (1975) Anther-derived haploids of the African violet. Can J Bot 53:1442–1444

    Google Scholar 

  • Iyer RD, Raina SK (1972) The early ontogeny of embryoids and callus from pollen and subsequent organogenesis in anther cultures of Datura metel and rice. Planta 104:146–156

    Google Scholar 

  • Jensen CJ (1977) Monoploid production by chromosome elimination. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 299–340

    Google Scholar 

  • Kameya T, Hinata K (1970) Induction of haploid plants from pollen grains of Brassica. Jpn J Breed 20:82–87

    Google Scholar 

  • Kasha KJ (ed) (1974) Haploids in higher plants: Advances and potential. Guelph Univ, Guelph

    Google Scholar 

  • Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare L.). Nature (London) 225:874–875

    CAS  Google Scholar 

  • Kasperbauer M J, Collins GB (1972) Reconstitution of diploids from leaf tissue of anther-derived haploids in tobacco. Crop Sci 12:98–101

    Google Scholar 

  • Kasperbauer M J, Buckner RC, Springer WD (1980) Haploid plants by anther-panicle culture of tall fescue. Crop Sci 20:103–106

    Google Scholar 

  • Keller WA, Armstrong KC (1977) Embryogenesis and plant regeneration in Brassica napus anther cultures. Can J Bot 55:1383–1388

    Google Scholar 

  • Keller WA, Armstrong KC (1978) High frequency production of microspore-derived plants from Brassica napus anther cultures. Z. Pflanzenzücht 80:100–108

    Google Scholar 

  • Keller WA, Rajhathy T, Lacapra J (1975) In vitro production of plants from pollen in Brassica campestris. Can J Genet Cytol 17:655–666

    CAS  Google Scholar 

  • Kermicle JL (1973) Androgenesis and the indeterminate gametophyte mutation: source of the cytoplasm. Maize Genet Coop Newslett 47:208–209

    Google Scholar 

  • Kimato M, Sakamoto S (1972) Production of haploid albino plants of Aegilops by anther culture. Jpn J Genet 47:61–63

    Google Scholar 

  • Kochbar T, Sabharwal P, Engelberg J (1971) Production of homozygous diploid plants by tissue culture technique. J Hered 62:59–61

    Google Scholar 

  • Kohlenbach HW, Geier T (1972) Embryonen aus in vitro kultivierten Antheren von Datura meteloides Dun., D. wrightii Regel und Solanum tuberosum L. Z Pflanzenphysiol 67:161–165

    Google Scholar 

  • Kuo JS, Wang YY, Chien NF, Ku SJ, Kung ML, Hsu UC (1973) Investigations on the anther culture in vitro of Nicotiana tabacum L. and Capsicum annuum L. Acta Bot Sinica 15:37–50

    Google Scholar 

  • LaRue CD (1954) Studies on growth and regeneration in gametophytes and sporophytes of gymnosperms. Brookhaven Symp Biol 6:187–208

    Google Scholar 

  • Li-Kung C (1978) Induction and culture of regenerated plantlets from anthers of genus Populus. Abstr. In: Proc Symp Plant Tissue Culture Peking. Science Press, Peking, p 241

    Google Scholar 

  • Maheshwari SC, Tyagi AK, Malhotra K, Sopory SK (1980) Induction of haploidy from pollen grains in angiosperms: The current status. Theor Appl Genet 58:193–206

    Google Scholar 

  • Malepszy ST, Grunewaldt J (1974) A contribution to the production of haploids in Hordeum vulgare L. Z Pflanzenzücht 72:206–211

    Google Scholar 

  • Maliga P, Marton L, Sz-Breznovits A (1973) 5-bromodeoxyuridine-resistent cell lines from haploid tobacco. Plant Sci Lett 1:119–121

    CAS  Google Scholar 

  • Mastrangelo IA, Smith HH (1977) Selection and differentiation of aminopterin resistant cells of Datura innoxia. Plant Sci Lett 10:171–179

    CAS  Google Scholar 

  • Melchers G (1972) Haploid higher plants for plant breeding. Z Pflanzenzücht 67:19–32

    Google Scholar 

  • Melchers G, Labib G (1970) Die Bedeutung haploider höherer Pflanzen für Pflanzenphysiologie und Pflanzenzüchtung. Ber Dtsch Bot Ges 83:129–150

    Google Scholar 

  • Melchers G, Labib G (1974) Somatic hybridization of plants by fusion of protoplasts. I. Selection of light-resistant hybrids of “haploid” light-sensitive varieties of tobacco. Mol Gen Genet 135:277–294

    Google Scholar 

  • Murakami M, Jakahashi N, Harada H (1973) Induction of haploid plant by anther culture in maize. I. Callus formation and root differentiation. Sci Rep Kyoto Prefect Univ Agric 24:1–8

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Nakamura A, Yamada T, Kadotani N, Itagaki R, Oka M (1974) Studies on the haploid method of breeding in tobacco. SABRAO J 6:107–131

    Google Scholar 

  • Nakata K, Tanaka M (1968) Differentiation of embryoids from developing germ cells in anther culture of tobacco. Jpn J Genet 43:65–71

    Google Scholar 

  • Narayanaswamy S, Chandy LP (1971) In vitro induction of haploid, diploid, and triploid androgenic embryoids and plantlets in Datura metel L. Ann Bot 35:535–542

    Google Scholar 

  • Narayanaswamy S, George L (1972) Morphogenesis of belladonna (Atropa belladonna L.) plantlets from pollen in culture. Indian J Exp Biol 10:382–384

    Google Scholar 

  • Narayanaswamy S, George L (1974) Experimental androgenesis in triploid and tetraploid Datura metel. Indian J Genet 34A:270–276

    Google Scholar 

  • Niizeki H, Oono K (1968) Induction of haploid rice plant from anther culture. Proc Jpn Acad 44:554–557

    Google Scholar 

  • Niizeki H, Oono K (1971) Rice plants obtained by anther culture. In: Colloq Int, CNRS 193. Les cultures de tissus de plantes, pp 251–257

    Google Scholar 

  • Niizeki M (1977) Haploid, polyploid and aneuploid plants from cultured anthers and calluses in species of Nicotiana and forage crops. J Fac Agric Hokkaido Univ 58:343–466

    Google Scholar 

  • Nishi T, Mitsuoka S (1969) Occurrence of various ploidy plants from anther and ovary culture of rice plant. Jpn J Genet 44:341–346

    Google Scholar 

  • Nitsch C (1974) La culture de pollen isolé sur milieu synthétique. CR Acad Sci Paris 278:1031–1034

    CAS  Google Scholar 

  • Nitsch C, Norreel B (1973) Effect d’un choc thermique sur le pouvoir embryogène due pollen de Datura innoxia cultivé dans l’anthèreou isolé de l’anthère. CR Acad Sci Paris 276:303–306

    Google Scholar 

  • Nitsch JP (1951) Growth and development in vitro of excised ovaries. Am J Bot 38:556–576

    Google Scholar 

  • Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404

    Google Scholar 

  • Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87

    PubMed  CAS  Google Scholar 

  • Nitsch JP, Nitsch C, Hamon S (1968) Réalisation expérimentale de 1’androgènese chez divers Nicotiana. CR Soc Biol 162:369–372

    Google Scholar 

  • Nitzsche W (1970) Herstellung haploider Pflanzen aus Festuca-Lolium Bastarden. Naturwissenschaften 57:199–200

    Google Scholar 

  • Nitzsche W, Wenzel G (1977) Haploids in plant breeding. Parey, Berlin Hamburg

    Google Scholar 

  • Ouyang T, Hu H, Chuang C, Tseng C (1973) Induction of pollen plants from anthers of Triticum aestivum L. cultured in vitro. Sci Sinica 16:79–90

    Google Scholar 

  • Pagniez M, Demarly Y (1979) Obtention d’individus androgenetiques par culture in vitro d’antheres de Ray-grass d’Italie (Lolium multiflorum Lam.). Ann Amelior Plantes 29:631–637

    Google Scholar 

  • Pandey KK (1973) Theory and practice of induced androgenesis. New Phytol 72:1129–1140

    Google Scholar 

  • Pandey KK, Couchman P, De Lautour G, Williams E (1975) A tissue culture technique for rapid clonal multiplication of androgenic haploids of tobacco. N Z J Bot 13:317–320

    Google Scholar 

  • Park SJ, Walsh EJ, Reinbergs E, Song LSP, Kasha KJ (1976) Field performance of doubled haploid barley lines in comparison with lines developed by the pedigree and single seed descent methods. Can J Plant Sci 56:467–474

    Google Scholar 

  • Pelletier G, Raquin C, Simon G (1972) La culture in vitro d’anthères d’asperge (Asparagus officinalis) CR Acad Sci Paris 274:848–851

    CAS  Google Scholar 

  • Picard E, De Buyser J (1975) New results on anther culture of wheat (Triticum aestivum L.) in vitro: conditions of regeneration of haploid plantlets and production of homozygous lines. CR Acad Sci Paris 281:989

    Google Scholar 

  • Preil W, Huhnke W, Engelhardt M, Hoffmann (1977) Haploide bei Gerbera jamesonii aus in vitro: Kulturen von Blutenkopfchen. Z. Pflanzenzücht 79:167–171

    Google Scholar 

  • Radojevic L (1978) In vitro induction of androgenic plantlets in Aesculus hippocastanum. Protoplasma 96:369–374

    Google Scholar 

  • Raghavan V (1975) Induction of haploid plants from anther cultures of henbane. Z Pflanzenphysiol 76:89–92

    Google Scholar 

  • Raina SK, Iyer RD (1973) Differentiation of diploid plants from pollen callus in anther cultures of Solanum melongena. Z Pflanzenzücht 70:275–280

    Google Scholar 

  • Raquin C, Pilet V (1972) Production de plantules à partir d’anthères de Pétunias cultivées in vitro. CR Acad Sci Paris 274:1019–1022

    Google Scholar 

  • Rashid A, Street HE (1973) The development of haploid embryoids from anther cultures of Atropa belladonna L. Planta 113:263–270

    Google Scholar 

  • Rashid A, Street HE (1974) Segmentation in microspores of Nicotiana sylvestris and Nicotiana tabacum which lead to embryoid formation in anther cultures. Protoplasma 80:323–334

    Google Scholar 

  • Reinbergs E, Song LSP, Choo TM, Kasha KJ (1978) Yield stability of double haploid lines of barley. Can J Plant Sci 58:929–933

    Google Scholar 

  • Reinert J, Bajaj YPS (1977) Anther culture: Haploid production and its significance. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue and organ culture. Springer, Berlin Heidelberg New York, pp 251–340

    Google Scholar 

  • Reinert J, Bajaj YPS, Heberle E (1975) Induction of haploid tobacco plants from isolated pollen. Protoplasma 84:191–196

    Google Scholar 

  • Rosati P, Devreux M, Laneri U (1975) Anther culture in strawberry. Hort Sci 10:119–120

    Google Scholar 

  • Sangwan RS, Norreel B (1975) Induction of plants from pollen grains of Petunia cultured in vitro. Nature (London) 257:222–224

    CAS  Google Scholar 

  • Schieder O (1978) Haploids from Datura innoxia as a tool for the production of homozygous lines with high content of scopolamine and for induction of mutants. In: Alfermann AW, Reinhard E (eds) Production of natural compounds by cell culture methods. Proc Int Symp Plant Cell Culture, Munich, pp 330–336

    Google Scholar 

  • Scowcroft WR (1977) Somatic cell genetics and plant improvement. Adv Agron 29:39–81

    CAS  Google Scholar 

  • Sharp WR, Raskin RS, Sommer HE (1972) The use of nurse culture in the development of haploid clones in tomato. Planta 104:357–361

    Google Scholar 

  • Sharp WR, Caldas LS, Crocomo OJ (1973) Studies on the induction of Coffea arabica callus from both somatic and microsporogenous tissue, and subsequent embryoid and plantlet formation. Am J Bot 60:13

    Google Scholar 

  • Sheldrake AR (1973) The production of hormones in higher plants. Biol Rev 48:509–559

    CAS  Google Scholar 

  • Sink KC, Padmanabhan V (1977) Anther and pollen culture to produce haploids: progress and application for the plant breeder. Hort Sci 12:143–148

    Google Scholar 

  • Song LSP, Park S J, Reinbergs E, Choo TM, Kasha KJ (1978) Doubled haploid vs the bulk plot method for production of homozygous lines in barley. Z Pflanzenzücht 81:271–280

    Google Scholar 

  • Stow I (1930) Experimental studies on the formation of the embryo sac-like giant pollen grain in the anther of Hyacinthus orientalis. Cytologia 1:417–439

    Google Scholar 

  • Sun CS, Wang CC, Chu CC (1973) Cytological studies on the androgenesis of Triticale. Acta Bot Sinica 15:163–173

    Google Scholar 

  • Sunderland N (1970) Pollen plants and their significance. New Sci 47:142–144

    Google Scholar 

  • Sunderland N (1971) Anther culture: a progress report. Sci Prog Oxford 59:527–549

    Google Scholar 

  • Sunderland N (1973) Pollen and anther culture. In: Street HE (ed) Plant tissue and cell culture. Blackwell, Oxford, pp 205–239

    Google Scholar 

  • Sunderland N (1974) Anther culture as a means of haploid induction. In: Kasha KJ (ed) Haploids in higher plants: Advances and potential. Guelph Univ, Guelph, pp 91–122

    Google Scholar 

  • Sunderland N (1977) Observations on anther culture of ornamental plants. In: Gautheret RJ (ed) La culture des tissues et des cellules de végétaux. Travaux dédiés à la mémoire de Georges Morel. Masson, Paris, pp 34–46

    Google Scholar 

  • Sunderland N (1978) Strategies in the improvement of yields in anther culture. In: Proc Plant Tissue Culture, Peking, pp 65–86

    Google Scholar 

  • Sunderland N, Roberts M (1977) A new approach to pollen culture. Nature (London) 270:236–238

    Google Scholar 

  • Sunderland N, Wicks FM (1969) Cultivation of haploid plants from tobacco pollen. Nature (London) 224:1227–1229

    Google Scholar 

  • Sunderland N, Wicks FM (1971) Embryoid formation in pollen grains of Nicotiana tabacum. J Exp Bot 22:213–226

    Google Scholar 

  • Sunderland N, Collins GB, Dunwell JM (1974) The role of nuclear fusion in pollen embryo-genesis of Datura innoxia Mill. Planta 117:227–241

    Google Scholar 

  • Thomas E, Wenzel W (1975) Embryogenesis from microspores of Brassica napus. Z Pflanzenzücht 74:77–81

    Google Scholar 

  • Thomas E, Hoffmann V, Wenzel G (1975) Haploid plantlets from microspores of rye. Z Pflanzenzücht 75:215–224

    Google Scholar 

  • Vasil IK (1980) Androgenetic haploids. In: Vasil IK (ed) Perspectives in plant cell and tissue culture. Int Rev Cytol, Suppl 11A. Academic Press, New York London, pp 195–223

    Google Scholar 

  • Vasil IK, Nitsch C (1975) Experimental production of haploids and their uses. Z Pflanzenphysiol 76:191–212

    Google Scholar 

  • Wagner G, Hess D (1974) Haploide, diploide, und triploide Pflanzen von Petunia hybrida aus Pollenkörnern. Z Pflanzenphysiol 73:273–276

    Google Scholar 

  • Wang CC, Chu CC, Sun CS, Wu SH, Yin KC, Hsu C (1973) The androgenesis in wheat (Triticum aestivum) anthers cultured in vitro. Sci Sinica 16:218–222

    Google Scholar 

  • Wang YY, Sun CS, Wang CC, Chien NF (1973) The induction of pollen plantlets of Triticale and Capsicum annuum from anther culture. Sci Sinica 16:147–151

    Google Scholar 

  • Wenzel G (1978) Production of haploids of rape and rye. In: Alfermann AW, Reinhard E (ed) Production of natural compounds by cell culture methods. Proc Int Symp Plant Cell Culture, Munich, pp 312–318

    Google Scholar 

  • Wenzel G (1979) Recent progress in microspore culture of crop plants. In: Proc 4th John Innes Symp, pp 185–196

    Google Scholar 

  • Wenzel G, Hoffmann F, Thomas E (1977) Increased induction and chromosome doubling of androgenetic haploid rye. Theor Appl Genet 51:81–86

    Google Scholar 

  • Wenzel G, Thomas E (1974) Observations on the growth in culture of anthers of Secale cereale. Z Pflanzenzücht 72:89–94

    Google Scholar 

  • Wernicke W, Kohlenbach HW (1976) Investigations on liquid culture medium as a means of anther culture in Nicotiana. Z Pflanzenphysiol 79:189–198

    Google Scholar 

  • Wernicke W, Kohlenbach HW (1977) Versuch zur Kultur isolierter Microsporen von Nicotiana und Hyoscyamus. Z Pflanzenphysiol 81:330–340

    Google Scholar 

  • Wernicke W, Harms CT, Lorz H, Thomas E (1978) Selective enrichment of embryogenic microspore populations. Naturwissenschaften 65:540–541

    Google Scholar 

  • White PR (1963) The cultivation of animal and plant cells. 2nd edition. Ronald Press, New York

    Google Scholar 

  • Woo SC, Huang CY (1980) Anther culture of Oryza glaberrima Steud. and its hybrids with Oryza sativa L. Bot Bull Academia Sinica 21:75–79

    Google Scholar 

  • Woo SC, Tung IJ (1972) Induction of rice plants from hybrid anthers of indica and japonica cross. Bot Bull Acad Sinica 13:67–69

    Google Scholar 

  • Zenkteler M (1971) In vitro production of haploid plants from pollen grains of Atropa belladonna L. Experientia 27:1087

    Google Scholar 

  • Zenkteler M (1972) Development of embryos and seedlings from pollen grains in Lycium halimifolium Mill. in the in vitro culture. Biol Plant Prague 14:420–422

    Google Scholar 

  • Zenkteler M (1973) In vitro development of embryos and seedlings from pollen grains of Solanum dulcamara. Z Pflanzenphysiol 69:189–192

    Google Scholar 

  • Zenkteler M, Misiura E (1974) Induction of androgenic embryos from cultured anthers of Hordeum, Secale, and Festuca. Biochem Physiol Pflanz 165:337–340

    Google Scholar 

  • Zenkteler M, Misiura E, Ponitka A (1975) Induction of androgenetic embryoids in the in vitro cultured anthers of several species. Experientia 31:289–291

    Google Scholar 

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Authors
  1. S. Narayanaswamy
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  2. L. George
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Editors and Affiliations

  1. Department of Botany, University of Delhi, Delhi, 110007, India

    Brij Mohan Johri

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© 1982 Springer-Verlag Berlin Heidelberg

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Narayanaswamy, S., George, L. (1982). Anther Culture. In: Johri, B.M. (eds) Experimental Embryology of Vascular Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67798-4_5

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  • DOI: https://doi.org/10.1007/978-3-642-67798-4_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-67800-4

  • Online ISBN: 978-3-642-67798-4

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