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Factors Modulating Mutagenicity in Microbial Tests

  • Conference paper
Short-Term Test Systems for Detecting Carcinogens

Summary

The preincubation of test compound, bacterial tester strain, and S-9 mix or buffer before pouring a minimal-glucose agar plate enhanced the sensitivity of mutation test and increased the spectrum of mutagens detected. Addition of NADH and ATP in S-9 mix enhanced the mutagenicity of some compounds. Addition of norharman in the preincubation mixture made it possible to detect a marginal or weak mutagenicity of certain types of mutagens. Addition of riboflavin revealed the mutagenicity of azo compounds. Glycosidase was required to detect the mutagenicity of glycosides or natural products.

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Author information

Authors and Affiliations

  1. Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Shirokane-dai, Minato-ku, Tokyo, 108, Japan

    T. Matsushima, A. Shirai & M. Sawamura

  2. Biochemistry Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, 104, Japan

    T. Sugimura, M. Nagao & T. Yahagi

Authors
  1. T. Matsushima
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  2. T. Sugimura
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  3. M. Nagao
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  4. T. Yahagi
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  5. A. Shirai
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  6. M. Sawamura
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Editor information

Editors and Affiliations

  1. Institut für Staublungenforschung und Arbeitsmedizin, Westfälischen Wilhelms-Universität, Westring 10, 4400, Münster, Germany

    K. H. Norpoth

  2. Cancer Research Unit, University of York, Heslington, York, YO15DD, Great Britain

    R. C. Garner

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© 1980 Springer-Verlag Berlin Heidelberg

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Matsushima, T., Sugimura, T., Nagao, M., Yahagi, T., Shirai, A., Sawamura, M. (1980). Factors Modulating Mutagenicity in Microbial Tests. In: Norpoth, K.H., Garner, R.C. (eds) Short-Term Test Systems for Detecting Carcinogens. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67202-6_20

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  • DOI: https://doi.org/10.1007/978-3-642-67202-6_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-67204-0

  • Online ISBN: 978-3-642-67202-6

  • eBook Packages: Springer Book Archive

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