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Technical Advances in Protoplast Isolation and Cultivation

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Plant Tissue Culture and Its Bio-technological Application

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Isolated plant protoplasts have become an important experimental system in some physiological, biochemical and genetic works. However, many experimental protoplast systems have proved to be limited by one or more technical problems, requiring extensive and sometimes fruitless investigation. This review focuses on technical problems and recent advances in isolation and cultivation of protoplasts. Earlier reviews have focused on different aspects of the handling of plant protoplasts. (Cocking, 1972; Gamborg et al., 1973; Eriksson et al., 1974). The first International Symposium on Higher Plant Protoplasts in Versailles, 1972, was convened to discuss significant advances and the basic difficulties in handling isolated protoplasts for cell transformation and cell fusion (Tempe, 1973). Vasil (1976) has in his review focused on progress, problems and prospects of plant protoplast research.

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References

  • Arnold, von, S., Eriksson, T.: Factors influencing the growth and division of Pea mesophyll protoplasts. Physiol. Plantariam 36, 193–196 (1976)

    Article  Google Scholar 

  • Binding, H.: Cell cluster formation by leaf protoplasts from axenic cultures of haploid Petunia hybrida L. Plant Sci. Lett. 2, 185–188 (1974)

    Article  Google Scholar 

  • Binding, H.: Reprodercibly high plating efficiencies of isolated mesophyll protoplasts from shoot cultures of tobacco. Physiol. Plantarum 35, 225–227 (1975)

    Article  Google Scholar 

  • Cocking, E.C.: Plant cell protoplasts-isolation and development. Ann. Rev. Plant Physiol. 23, 29–50 (1972)

    Article  CAS  Google Scholar 

  • Constabel, F.: Isolation and culture of plant protoplasts. In: Plant Tissue Culture Methods. Gamborg, O.L., Wetter, L.R. (eds.). Ottawa : National Research Council of Canada, 1975, pp. 11–22

    Google Scholar 

  • Constabel, F., Kirkpatrick, J.W., Gamborg, O.L.: Callus formation from mesophyll protoplasts of Pisum sativum. Can. J. Botany 51, 2105–2106 (1973)

    Article  Google Scholar 

  • Coutts, R.H.A., Wood, K.R.: The isolation and culture of cucumber mesophyll protoplasts. Plant Sci. Lett. 4, 189–193 (1975)

    Article  Google Scholar 

  • Dudits, D., Kao, K.N., Constabel, F., Gamborg, O.L.: Embryo-genesis and formation of tetraploid and hexaploid plants from carrot protoplasts. Can. J. Botany 54, 1063–1064 (1976)

    Article  CAS  Google Scholar 

  • Eriksson, T., Bonnett, H., Glimelius, K., Wallin, A.: Technical advances in protoplast isolation, culture and fusion. In: Tissue Culture and Plant Science. Street, H.E. (ed.). New York: Academic Press, 1974, pp. 213–231

    Google Scholar 

  • Gamborg, O.L., Kao, K.N., Miller, R.A., Fowke, L.C., Constabel, F.: Cell regeneration, division and plant development from protoplasts. Coll. Intern. Cent. Natl. Rech. Sci. 212; pp. 155–173, 1973

    Google Scholar 

  • Gamborg, O.L., Shyluk, J., Kartha, K.K.: Factors affecting the isolation and callus formation in protoplasts from the shoot apices of Visum sativum. Plant Sci. Lett. 4, 285–292 (1975)

    Article  CAS  Google Scholar 

  • Glimelius, K., Wallin, A., Eriksson, T.: Agglutinating effects of Concanavalin A on isolated protoplasts of Daucus carota. Physiol. Plant 30, 564–569 (1974)

    Google Scholar 

  • Howland, G.P., Hart, R.W.: Radiation biology of cultured plant cells. In: Applied and Fundamental Aspects of Plant Tissue Culture. Reinert, J., Bajaj, Y.P.S. (eds.). Berlin: Springer, 1976

    Google Scholar 

  • Kameya, T.: Culture of protoplasts from chimeral plant tissue of nature. Jap. J. Genetics 50 (5), 417–420 (1975)

    Article  Google Scholar 

  • Kanai, R., Edwards, G.E.: Purification of enzymatically isolated mesophyll protoplasts from C3, C4 and crassulacean acid metabolism plants using an aqueous dextran-poly-ethylene glycol two-phase system. Plant Physiol. 52. 484–490 (1973)

    Article  PubMed  CAS  Google Scholar 

  • Kao, K.N., Gamborg, O.L., Michayluk, M.R., Keller, W.A., Miller, R.A.: The effect of sugars and inorganic salts on cell regeneration and sustained division in plant protoplasts. C.N.R.S. 212, 207–213 (1973)

    Google Scholar 

  • Kao, K.N., Gamborg, O.L., Miller, R.A., Keller, W.A.: Cell division in cells regenerated from soybean and Haplopappus gracilis. Nature (New Biol.) 232, 124 (1971)

    Article  Google Scholar 

  • Kao, K.N., Michayluk, M.R.: Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126, 105–110 (1975)

    Article  CAS  Google Scholar 

  • Kartha, K.K., Michayluk, M.R., Kao, K.N., Gamborg, O.L.: Callus formation and plant regeneration from mesophyll protoplasts of rape plants, Brassica naupus L. CV Zephyr. Plant Sci. Lett. 3, 265–271 (1974)

    Article  CAS  Google Scholar 

  • Larkin, P.J.: Purification and viability determinations of plant protoplasts. Planta 128, 213–216 (1976)

    Article  Google Scholar 

  • Meyer, Y.: Isolation and culture of tobacco mesophyll protoplasts using a saline medium. Protoplasma 81, 363–372 (1974)

    Article  Google Scholar 

  • Nagata, T., Takebe, I.: Plating of isolated tobacco mesophyll protoplast on sugar medium. Planta 99, 12–20 (1971)

    Article  Google Scholar 

  • Norstog, K.: New synthetic medium for the culture of premature barley embryos. In Vitro 8, 307–308 (1973)

    Article  PubMed  CAS  Google Scholar 

  • Ohyama, K., Nitsch, J.P.: Flowering haploid plants obtained from protoplasts of tobacco leaves. Plant Cell Physiol. 13, 229–236 (1972)

    Google Scholar 

  • Pelcher, L.E., Gamborg, O.L., Kao, K.N.: Bean mesophyll protoplasts: production, culture and callus formation. Plant Sci. Lett. 3, 107–111 (1974)

    Article  Google Scholar 

  • Poirier-Hamon, S., Rao, P.S., Harada, H.: Culture of mesophyll protoplasts and stem segments of Antirrhinum majus (snapdragon): Growth and Organization of Embryoids. J. Exptl. Botany 25 (87), 752–760 (1974)

    Article  CAS  Google Scholar 

  • Raj, B., Herr, J.M.: Isolation of protoplasts from the placental cells of Lycopersicum pimpinellifolium Mill. Exptl. Cell. Res. 64, 479–480 (1970)

    Article  Google Scholar 

  • Shepard, J.F., Totten, R.E.: Isolation and regeneration of tobacco mesophyll cell protoplasts under low osmotic conditions. Plant Physiol. 55, 689–694 (1975)

    Article  PubMed  CAS  Google Scholar 

  • Taiz, L., Jones, R.L.: The isolation of barley-aleurone protoplasts. Planta 101, 95–100 (1971)

    Article  CAS  Google Scholar 

  • Takebe, I., Nagata, T.: Culture of isolated tobacco mesophyll protoplasts. In: In Protoplastes et Fusion de Cellules Somatiques Vegetales. Tempé, J. (ed.) . Paris: Inst. Nat. Roch. Agron, 1973, pp. 175–187

    Google Scholar 

  • Takebe, I., Otsuki, Y., Aoki, S.: Isolation of tobacco mesophyll cells in intact and active state. Plant Cell Physiol. 9, 115–124 (1968)

    Google Scholar 

  • Uchimiya, H., Murashige, T.: Influence of the nutrient medium on the recovery of dividing cells from tobacco protoplasts. Plant Physiol. 57, 424–429 (1976)

    Article  PubMed  CAS  Google Scholar 

  • Upadhya, M.D.: Isolation and culture of mesophyll protoplasts of potato (Solanum tuberosum L.) Potato Res. 18, 438–445 (1975)

    Article  Google Scholar 

  • Wallin, A., Glimelius, K., Eriksson, T.: The induction of aggregation and fusion of Daucus carota protoplasts by polyethylene glycol. Z. Pflanzenphysiol. 74, 64–80 (1974)

    CAS  Google Scholar 

  • Wallin, A., Eriksson, T.: Protoplast cultures from cell suspensions of Daucus carota. Physiol. Plantarum 28, 33–39 (1973)

    Article  Google Scholar 

  • Wakasa, K.: Isolation of protoplasts from various plant organs. Jap. J. Genetics 40, 279–289 (1973)

    Article  Google Scholar 

  • Vasil, I.K.: The progress, problems and prospects of plant protoplast research. In: Advances in Agronomy. Brady N.C. (ed.). New York: Academic Press, 1976, Vo. XXVIII, 28 (1976)

    Google Scholar 

  • Watts, J.W., Motoyoshi, F., King, J.M.: Problems associated with the production of stable protoplasts of cells of tobacco mesophyll. Ann. Botany 38, 667–671 (1974)

    Google Scholar 

  • Widholm, J.M.: The use of fluorescein diccetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 47, 189–194 (1972)

    PubMed  CAS  Google Scholar 

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Authors
  1. T. Eriksson
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Editors and Affiliations

  1. Lehrstuhl für Biochemie der Pflanzen, Westfälische Wilhelms-Universität, 4400, Münster, Germany

    W. Barz

  2. Lehrstuhl für Pharmazeutische Biologie der Universität Auf der Monrgenstelle, 7400, Tübingen, Germany

    E. Reinhard

  3. Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, 4630, Bochum-Querenburg, Germany

    M. H. Zenk

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© 1977 Springer-Verlag Berlin Heidelberg

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Eriksson, T. (1977). Technical Advances in Protoplast Isolation and Cultivation. In: Barz, W., Reinhard, E., Zenk, M.H. (eds) Plant Tissue Culture and Its Bio-technological Application. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66646-9_26

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  • DOI: https://doi.org/10.1007/978-3-642-66646-9_26

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-66648-3

  • Online ISBN: 978-3-642-66646-9

  • eBook Packages: Springer Book Archive

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