Abstract
Leukemic cell lines represent an essential instrument for the biological characterization of human leukemia. In contrast to primary cell preparations, almost unlimited cell quantities are available with relative ease. Established lymphoblasts form a homogeneous population of cells without contamination by normal leukocytes and in addition some experimental techniques (e.g. radioactive labelling) imply a proliferation in vitro. One has to ensure, however, that the in vitro cultivated lymphoblasts represent truly leukemic cells. In practice the overgrowth by Epstein-Barr-Virus (EBV)-transformed lymphocytes handicaps the establishment of leukemic cell lines and only a few of them have been described so far (1, 2, 3, 4). Encouraged by the fact that acute lymphoblastic leukemia (ALL) occurs most frequently in young children, and also that infection with EBV in this age group is less frequent than in adults, we started long term cultures with ALL derived cell preparations. Similarly we put lymphoblasts from children with leukemic transformation of a Non-Hodgkin lymphoma (NHL) into culture. As a result we obtained three leukemic cell lines and some of their characteristics are reported in this communication.
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© 1977 Springer-Verlag Berlin Heidelberg
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Schneider, U., Schwenk, H.U. (1977). Characterization of “T” and “Non-T” Cell Lines Established From Children With Acute Lymphoblastic Leukemia and Non-Hodgkin Lymphoma After Leukemic Transformation. In: Thierfelder, S., Rodt, H., Thiel, E. (eds) Immunological Diagnosis of Leukemias and Lymphomas. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 20. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66639-1_32
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DOI: https://doi.org/10.1007/978-3-642-66639-1_32
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