Abstract
The normally obtained photoreactivation (PhR) by continuous illumination raised three questions that could not be answered by the classical instrumental set-up:
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1.
How many molecules of the photoreactivating enzyme exist per cell?
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2.
What are the kinetics of PhR?
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3.
What is the action spectrum of PhR?
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References
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Harm, W., Rupert, C.S., Harm, H.: The study of photoenzymatic repair of UV lesions in DNA by flash photolysis. In: Photophysiology (Giese, A.C., ed.). New York: Academic Press, 1971, Vol. VI, pp. 279–324
Jagger, J., Stafford, R.S., Snow, J.M.: Thymine-dimer and action-spectrum evidence for indirect photoreactivation in Escherichia coli. Photochem. Photobiol. 10, 383–395 (1969)
Sutherland, J.C., Sutherland, B.M.: Human photoreactivating enzyme. Action spectrum and safelight conditions. Biophys. J. 15, 435–440 (1975)
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Anders, A., Yasui, A., Zacharias, H., Lamprecht, I., Laskowski, W. (1976). Evaluation of the Action Spectrum of Yeast Photoreactivation in vivo by Means of Pulsed Dye Lasers. In: Kiefer, J. (eds) Radiation and Cellular Control Processes. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66455-7_28
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DOI: https://doi.org/10.1007/978-3-642-66455-7_28
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