Skip to main content
SpringerLink
Log in

Growth of Biotrophic Parasites in Tissue Culture

  • Chapter
Physiological Plant Pathology

Part of the book series: Encyclopedia of Plant Physiology ((PLANT,volume 4))

Abstract

Plant tissue culture techniques were originally developed for the purpose of studying fundamental problems of nutrition and morphogenesis, and have made important contributions in these fields. In addition it has become increasingly clear that cells and tissues cultured under controlled aseptic conditions have an important role to play in other branches of plant biology, including pathology. They have already been extensively employed in studies of the crown gall disease caused by Agrobacterium tumefaciens (Butcher, 1973), and their potential uses in the study of other plant diseases are being explored and developed (Ingram, 1973). In the case of biotrophic parasites they provide a potential method for the maintenance of monoxenic cultures and offer the attractive possibility of a simplified experimental system for studies of some aspects of host-parasite interaction. By using plant tissue cultures large numbers of apparently uniform host cells may be exposed to a parasite without excessive tissue injury, cell number and inoculum density may be controlled with precision, and the precursors and products of metabolic interaction may be added or removed with comparative ease.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Albouy, J., Kusiak,C., Lapierre, H., Laquerrière, F., Maury,Y.: Isolement et propriétés de cellules de mésophylle de chou de Chine (Brassica chinensis) infectées par le virus de la mosaique jaune du navet. Ann. Phytopathol. 2, 607–616 (1970).

    Google Scholar 

  • Aoki, S., Takebe,I.: Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39, 439–448 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Augier de Montgremier, H., Morel, G.: Sur la diminution de la teneur en virus (Marmor tabaci Holmes) de tissus de tabac cultivés in vitro. Compt. Rend. 227, 688–689 (1948).

    Google Scholar 

  • Bailey, J.A.: Pisatin production by tissue cultures of Pisum sativum L. J. Gen. Microbiol. 61, 409–415 (1970).

    CAS  Google Scholar 

  • Beachy, R.N., Murakishi, H.H.: Local lesion formation in tobacco tissue culture. Phytopathology 61, 877–878 (1971a).

    Article  Google Scholar 

  • Beachy, R.N., Murakishi,H.H.: Proteins from cultured cells of Xanthi-nc tobacco inoculated with tobacco mosaic virus. Phytopathology 61, 884 (abstr.) (1971b).

    Article  Google Scholar 

  • Bingefors, S.: Resistance against stem nematodes Ditylenchus dipsaci (Kühn). Filipjev. EPPO Publications Ser. A No. 54, 63–75 (1970).

    Google Scholar 

  • Bingefors, S., Eriksson, K.B.: Some problems connected with resistance breeding against stem nematodes in Sweden. Z. Pflanzenzucht. 59, 359–375 (1968).

    Google Scholar 

  • Black, L.M.: Insect tissue cultures as tools in plant virus research. Ann. Rev. Phytopathol. 7, 73–100 (1969).

    Article  Google Scholar 

  • Braun, A.C., Lipetz,J.: The use of tissue culture in phytopathology. In: Cells and Tissues in Culture, vol. 3, p. 691–722 (E.N. Willmer, ed.). London-New York: Academic Press 1966.

    Google Scholar 

  • Brian, P.W.: Obligate parasitism in fungi. Proc. Roy. Soc. (London) Ser. B 168, 101–118 (1967).

    Article  CAS  Google Scholar 

  • Burgess, J., Motoyoshi, F., Fleming, E.N.: Effect of poly-L-ornithine on isolated tobacco mesophyll protoplasts: evidence against stimulated pinocytosis. Planta 111, 199–208 (1973a).

    Article  CAS  Google Scholar 

  • Burgess, J., Motoyoshi,F., Fleming, E.N.: The mechanism of infection of plant protoplasts by viruses. Planta 112, 323–332 (1973b).

    Article  Google Scholar 

  • Butcher, D.N.: The origins, characteristics and culture of plant tumour cells. In: Plant Tissue and Cell Culture, p. 356–391, (H.E. Street, ed.). Oxford-Edinburgh: Blackwell 1973.

    Google Scholar 

  • Butcher, D.N., Connolly, J.D.: An investigation of factors which influence the production of abnormal terpenoids by callus cultures of Andro graphis paniculata Nees. J. Exptl. Botany 22, 314–322 (1971).

    Article  CAS  Google Scholar 

  • Butcher, D.N., Street,H.E.: Excised root culture. Botan. Rev. 30, 513–586 (1964).

    Article  CAS  Google Scholar 

  • Carlson,P.S.: Methionine sulfoxamine-resistant mutants of tobacco. Science 180, 1366–1368 (1973a).

    Article  PubMed  CAS  Google Scholar 

  • Carlson, P.S.: The use of protoplasts for genetic research. Proc. Natl. Acad. Sci. U.S. 70, 598–602 (1973b).

    Article  CAS  Google Scholar 

  • Chandra, N., Hildebrandt, A.C.: Differentiation of plants from TMV inclusion-bearing and inoculum-free tobacco cells. Virology 31, 414–421 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Cocking, E.C.: An electron microscopic study of the initial stages of infection of isolated tomato fruit protoplasts by tobacco mosaic virus. Planta 68, 206–214 (1966).

    Article  Google Scholar 

  • Cocking, E.C.: Virus uptake, cell wall regeneration and virus multiplication in isolated plant protoplasts. Intern. Rev. Cytol. 28, 89–124 (1970).

    Article  CAS  Google Scholar 

  • Cocking, E.C: Plant cell protoplasts —isolation and development. Ann. Rev. Plant Physiol. 23, 29–50 (1972).

    Article  CAS  Google Scholar 

  • Cruse, P.F., Patterson, M.K. (ed.): Tissue Culture: Methods and Applications. London-New York: Academic Press 1973.

    Google Scholar 

  • Coutts, R.H.A., Cocking, E.C., Kassanis, B.: Infection of tobacco mesophyll protoplasts with TMV. J. Gen. Virol. 17, 289–294 (1973).

    Article  Google Scholar 

  • Cutter, V.M.: Studies on the isolation and growth of plant rusts in host tissue cultures and upon synthetic media. I Gymnosporangium. Mycologia 51, 248–295 (1959).

    Article  Google Scholar 

  • Cutter, V.M.: Studies on the isolation and growth of plant rusts in host tissue cultures and upon synthetic media. II Uromyces ari-triphylli. Mycologia 52, 726–742 (1960).

    Article  Google Scholar 

  • Dekhuijzen, H.M., Overeem, J.C: The role of cytokinins in clubroot formation. Physiol. Plant Pathol. 1, 151–162 (1971).

    Article  CAS  Google Scholar 

  • Francki, R.I.B., Zaitlin,M., Jensen, R.G.: Metabolism of separated leaf cells. II Uptake and incorporation of protein and RNA precursors by tobacco cells. Plant Physiol. 48, 14–18 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Friend, J.: The resistance of potato to Phytophthora. In: Fungal Pathogenicity and the Plant’s Response, p. 383–399, (R.J.W. Byrde and C.V. Cutting, eds.). London-New York: Academic Press 1973.

    Google Scholar 

  • Gamborg, O.L., Wetter, L.R. (eds.): Plant Tissue Culture Methods. Saskatoon: National Research Council of Canada 1975.

    Google Scholar 

  • Gautheret, R.J.: La culture des tissus végétaux. Paris: Masson 1959.

    Google Scholar 

  • Harvey, A.E., Grasham, J.L.: Growth of Cronartium ribicola in the absence of physical contact with its host. Canad. J. Botany 48, 71–73 (1970).

    Article  Google Scholar 

  • Harvey, A.E., Grasham, J.L.: Inoculation of a non-host tissue culture with Cronartium ribicola. Canad. J. Botany 49, 881–882 (1971).

    Article  Google Scholar 

  • Heinz, D.J., Mee, G.W.P.: Morphological, cytogenetic and enzymatic variation in Saccharum species hybrid clones derived from callus tissue. Am. J. Botany 58, 257–262 (1971).

    Article  Google Scholar 

  • Helgeson, J.P., Kemp, J.D., Haberlach, G.T., Maxwell, D.P.: A tissue culture system for studying disease resistance: the black shank disease in tobacco callus cultures. Phytopathology 62, 1439–1443 (1972).

    Article  Google Scholar 

  • Hildebrandt, A.C.: Growth and differentiation of single plant cells and tissues. In: Les cultures de tissues de plantes, p. 71–93, Colloques Internationaux C.N.R.S. No. 193 (1971).

    Google Scholar 

  • Hirth, L., Durr, A.: Données récentes sur la multiplication du virus de la mosaique du tabac dans des tissus de tabac cultivés in vitro. In: Les cultures de tissus de plantes, p. 481–498, Colloques Internationaux C.N.R.S. No. 193 (1971).

    Google Scholar 

  • Hollings, M.: Disease control through virus-free stock. Ann. Rev. Phytopathol. 3, 367–396 (1965).

    Article  Google Scholar 

  • Ingram, D.S.: The expression of R-gene resistance to Phytophthora infestans in tissue cultures of Solanum tuberosum. J. Gen. Microbiol. 49, 99–108 (1967).

    PubMed  CAS  Google Scholar 

  • Ingram, D.S.: Growth of Plasmodiophora brassicae in host callus. J. Gen. Microbiol. 55, 9–18 (1969a).

    PubMed  CAS  Google Scholar 

  • Ingram, D.S.: Abnormal growth of tissues infected with Plasmodiophora brassicae. J. Gen. Microbiol. 56, 55–67 (1969b).

    PubMed  CAS  Google Scholar 

  • Ingram, D.S.: The susceptibility of Brassica callus to infection by Peronospora parasitica. J. Gen. Microbiol. 58, 391–401 (1969c).

    Google Scholar 

  • Ingram, D.S.: Growth of plant parasites in tissue culture. In: Plant Tissue and Cell culture, p. 392–421, (H.E. Street, ed.). Oxford-Edinburgh: Blackwell 1973.

    Google Scholar 

  • Ingram, D.S., Tommerup, I.C.: The study of obligate parasites in vitro. In: Fungal Pathogenicity and the Plant’s Response, p. 121–140, (R.J.W. Byrde, C.V. Cutting, eds.). London-New York: Academic Press 1973.

    Google Scholar 

  • Jackson, A.O., Zaitlin, M., Siegel, A., Francki, R.I.B.: Replication of tobacco mosaic virus. III Viral RNA metabolism in separated leaf cells. Virology 48, 655–665 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Johnson, R.N., Viglierchio, D.R.: Sugar beet nematode (Heterodera schachtii) reared on axenic Beta vulgaris root expiants. I Selected environmental factors affecting penetration. Nematologica 15, 129–143 (1969a).

    Article  Google Scholar 

  • Johnson, R.N., Viglierchio, D.R.: Sugar beet nematode (Heterodera schachtii) reared on axenic Beta vulgaris root expiants. II Selected environmental and nutritional factors affecting development and sex ratio. Nematologia 15, 144–152 (1969b).

    Article  Google Scholar 

  • Kassanis, B.: Plant tissue culture. In: Methods in Virology, vol. 1, p. 537–566, (K. Maramorosch, H. Koprowski, eds.). London-New York: Academic Press 1967.

    Google Scholar 

  • Keen, N.T., Horsch, R.: Hydroxyphaseollin production by various soybean tissues: a warning against use of “unnatural” host-parasite systems. Phytopathology 62, 439–442 (1972).

    Article  Google Scholar 

  • Keen, N.T., Reddy, M.N., Williams, P.H.: Isolation and properties of Plasmodiophora brassicae plasmodia from infected crucifer tissue and from tissue-culture callus. Phytopathology 59, 637–644 (1969).

    Google Scholar 

  • Krikorian, A.D., Steward, F.C.: Biochemical differentiation: the biosynthetic potentialities of growing and quiescent tissue. In: Plant Physiology, vol. V.B., p. 227–326, (F.C. Steward, ed.). New York: Academic Press 1969.

    Google Scholar 

  • Krishnamurthi, M., Tlaskal, J.: Fiji disease-resistant Saccharum officinarum var. Pindar sub-clones from tissue cultures. Proc. Intern. Soc. Sugar-Cane Technologists (Plant Breeding Section), 1–8 (1974).

    Google Scholar 

  • Maheshwari, R.: Applications of plant tissue and cell culture in the study of physiology of parasitism. Proc. Indian Acad. Sci. Sect. B 59, 152–172 (1969).

    Google Scholar 

  • Maheshwari, R., Hildebrandt, A.C., Allen, P.J.: Factors affecting the growth of rust fungi on host tissue cultures. Botan. Gaz. 128, 153–159 (1967).

    Article  Google Scholar 

  • Morel, G.: Recherches sur la culture associée de parasites obligatoires et de tissus végétaux. Ann. Épiphyties 14, 1–112 (1948).

    Google Scholar 

  • Mountain, W.B.: A method of culturing plant parasitic nematodes under sterile conditions. Proc. Helminthol. Soc. Wash. D.C. 22, 49–52 (1955).

    Google Scholar 

  • Murakishi, H.H., Hartman, J.X., Beachy, R.N., Pelcher, L.E.: Growth curve and yield of tobacco mosaic virus in tobacco callus cells. Virology 43, 62–68 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Murakishi, H.H., Hartman, J.X., Pelcher,L.E., Beachy, R.N.: Improved inoculation of cultured plant cells resulting in high virus titre and crystal formation. Virology 41, 365–367 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Murashige, T.: Plant propagation through tissue culture. Ann. Rev. Plant Physiol. 25, 135–166 (1974).

    Article  CAS  Google Scholar 

  • Otsuki, Y., Shimomura,T., Takebe, I.: Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties. Virology 50, 45–50 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Otsuki, Y., Takebe,I., Honda, Y., Matsui, C.: Ultrastructure of infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Virology 49, 188–194 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Pelcher, L.E., Murakishi, H.H., Hartman,J.X.: Kinetics of TMV-RNA synthesis and its correlation with virus accumulation and crystalline viral inclusion formation in tobacco tissue cultures. Virology 47, 787–796 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Raychaudhuri, S.P.: Plant viruses in tissue culture. Adv. Virus Res. 12, 175–206 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Reinert, R.A.: Virus activity and callus growth of infected and healthy callus tissues of Nicotiana tabacum grown in vitro. Phytopathology 56, 731–733 (1966).

    Google Scholar 

  • Robertson, N.F., Friend, J., Aveyard, M., Brown,J., Huffee, M., Homans, A.L.: The accumulation of phenolic acids in tissue culture pathogen combinations of Solanum tuberosum and Phytophthora infestons. J. Gen. Microbiol. 54, 261–268 (1968).

    PubMed  CAS  Google Scholar 

  • Sakai, F., Takebe,I.: RNA and protein synthesis in protoplasts isolated from tobacco leaves. Biochim. Biophys. Acta 224, 531–540 (1970).

    PubMed  CAS  Google Scholar 

  • Sakai, F.,Takebe, I.: A non-coat protein synthesized in tobacco mesophyll protoplasts infected by tobacco mosaic virus. Molec. Gen. Genet. 118, 93–96 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Samaddar, K.R., Scheffer, R.P.: Effect of the specific toxin in Helminthosporium victoriae on host cell membranes. Plant Physiol. 43, 21–28 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Sandstedt, R., Schuster, M.L.: The role of auxins in root knot nematode-induced growth on excised tobacco stem segments. Physiol. Plantarum 19; 960–967 (1966).

    Article  CAS  Google Scholar 

  • Sayre, R.M.: Plant Tissue Culture as a Tool in the Study of the Physiology of Root-knot Nematode Meloidogyne incognita Chit. Ph. D. Thesis, University of Nebraska, Lincoln, 1958.

    Google Scholar 

  • Shaw, J.G.: Effect of poly-L-ornithine on the attachment of tobacco mosaic virus to tobacco leaves and on uncoating of viral RNA. Virology 48, 380–385 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Siegel, A.,Zaitlin, M., Duda,C.T.: Replication of tobacco mosaic virus IV. Further characterization of viral related RNAs. Virology 53, 75–83 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Street, H.E. (ed.): Plant Tissue and Cell Culture. Oxford-Edinburgh: Blackwell 1973.

    Google Scholar 

  • Street, H.E. (ed.): Tissue Culture and Plant Science. London-New York: Academic Press 1974.

    Google Scholar 

  • Sunderland, N.: Anther culture: a progress report. Sci. Progr. (Oxford) 59, 527–549 (1971).

    Google Scholar 

  • Takebe, I.: The use of protoplasts in plant virology. Ann. Rev. Phytopathol. 13, 105–125 (1975).

    Article  Google Scholar 

  • Takebe, I., Otsuki, Y.: Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proc. Nat. Acad. Sci. U.S. 64, 843–848 (1969).

    Article  CAS  Google Scholar 

  • Takebe, I., Otsuki, Y., Aoki, S.: Infection of isolated tobacco mesophyll protoplasts by tobacco mosaic virus. In: Les cultures de tissus de plantes p. 503–511, Colloques Internationaux C.N.R.S. No. 193 (1971).

    Google Scholar 

  • Tiwari, M.M., Arya, H.C.: Sclerospora graminicola axenic culture. Science 163, 291–293 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Walkey, D.G.A., Cooper, V.C.: Some factors affecting the behaviour of plant viruses in tissue culture. Physiol. Plant Pathol. 2, 259–264 (1972).

    Article  Google Scholar 

  • Webster, J.M.: Production of oat callus and its susceptibility to a plant parasitic nematode. Nature 212, 1472 (1966).

    Article  CAS  Google Scholar 

  • Webster, J.M.: The influence of plant growth substances and their inhibitors on the host-parasite relationships of Aphelenchoides ritzemabosi in culture. Nematologica 13, 256–262 (1967a).

    Article  CAS  Google Scholar 

  • Webster, J.M.: Some effects of 2,4-D herbicides on nematode-infected cereals. Plant Pathol. 16, 23–26 (1967b).

    Article  CAS  Google Scholar 

  • Webster, J.M.,Lowe, D.: The effect of the synthetic plant growth substance, 2,4-dichlorophen-oxyacetic acid, on the host-parasite relationships of some plant-parasitic nematodes in monoxenic callus culture. Parasitology 56, 313–322 (1966).

    Article  PubMed  CAS  Google Scholar 

  • White, P.R.: The Cultivation of Animal and Plant Cells. London: Thames and Hudson 1954.

    Google Scholar 

  • White, P.R.: Tissue culture in the service of phytopathology. Indian Phytopathol. 21, 14–22 (1968).

    Google Scholar 

  • Williams, P.H.,Reddy, M.N., Strandberg, J.O.: Growth of non-infected and Plasmodiophora brassicae infected cabbage callus in culture. Canad. J. Botany 47, 1217–1221 (1969).

    Article  Google Scholar 

  • Willmer, E.N. (ed.): Cells and Tissues in Culture, vol. 3. London-New York: Academic Press 1966.

    Google Scholar 

  • Wu, J.H., Hildebrandt, A.C., Riker,A.J.: Virus-host relationships in plant tissue culture. Phytopathology 50, 587–594 (1960).

    Google Scholar 

  • Zaitlin, M., Beachy,R.N.: The use of protoplasts and separated cells in plant virus research. Advan. Virus Res. 19, 1–35 (1974a).

    Article  CAS  Google Scholar 

  • Zaitlin, M., Beachy, R.N.: Protoplasts and separated cells: some new vistas for plant virology. In: Tissue Culture and Plant Science (H.E. Street, ed.). London-New York: Academic Press 1974b.

    Google Scholar 

  • Zuckerman, B.M.: Gnotobiology. In: Plant Parasitic Nematodes, vol. II, p. 159–184, (B.M. Zuckerman, W.F. Mai, R.A. Rhodes, eds.). London-New York: Academic Press 1971.

    Google Scholar 

Download references

Authors
  1. D. S. Ingram
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Pflanzenpathologie und Pflanzenschutz der Universität, Grisebachstraße 6, 34 Göttingen, Federal Republic of Germany

    R. Heitefuss

  2. Madison, USA

    P. H. Williams

Rights and permissions

Reprints and permissions

Copyright information

© 1976 Springer-Verlag Berlin · Heidelberg

About this chapter

Cite this chapter

Ingram, D.S. (1976). Growth of Biotrophic Parasites in Tissue Culture. In: Heitefuss, R., Williams, P.H. (eds) Physiological Plant Pathology. Encyclopedia of Plant Physiology, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66279-9_28

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-66279-9_28

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-66281-2

  • Online ISBN: 978-3-642-66279-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation