Abstract
Vitamin A, which maintains cellular differentiation of epithelial tissues, also has an effect on metaplastic and neoplastic lesions in the respiratory tract. The surface morphology of hamster tracheal epithelium during vitamin A deficiency and during reversal upon the administration of vitamin A was studied by scanning electron microscopy. Focal squamous metaplasia of the tracheal epithelium, confined upon the upper two thirds of the ventral surface of the trachea, occurs in vitamin A deficiency. Synchronous differentiation of the epithelium with sloughing of the existing surface occurs after reversal of the deficient state. This change is rapid, occurring within 4 days of the administration of vitamin A. The SEM enabled documentation of extent distribution and surface changes in vitamin A deficiency and reversal.
This work was performed in part under Contract No. NIH-NCI-72-3292, Division of Cancer Cause and Prevention, National Cancer Institute, National Institutes of Health.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
ANDERSON, T.F.: Techniques for the preservation of three-dimensional structure in preparing specimens for the electron microscope. Trans. N.Y. Acad. Sci. Ser. II. 13, 130–134 (1951).
GREENWOOD, M.F., HOLLAND, P.: The mammalian respiratory tract surface. A scanning electron microscopic study. Lab. Invest. 27, 296–304 (1972).
GRONIOWSKI, J., WALSKI, M., BICZYSKO, W.: Application of scanning electron microscopy for studies of the lung parenchyma. J. Ultrastruct. Res. 38, 473–481 (1972).
HARRIS, C.C., SPORN, M.B., KAUFMAN, D.G., SMITH, J.M., JACKSON, F.E., SAFFIOTTI, U.: Histogenesis of squamous metaplasia in the hamster tracheal epithelium caused by vitamin A deficiency or benzo(a)pyrene ferric oxide. J. nat. Cancer Inst. 48, 743–761 (1972).
HOLMA, B.: Scanning electron microscopic observation of particles deposited in the lung. Arch. Environ. Hlth 18, 330–339 (1969).
KARNOVSKY, J.: A formaldehyde-gluteraldehyde fixative of high osmololity for use in electron microscopy. J. Cell Biol. 27, 137A (1965).
KUHN, C., FINKE, E.H.: The topography of the pulmonary alveolus: Scanning electron microscopy using different fixations. J. Ultra-struct. Res. 38, 161–173 (1972).
LUPULESCU, A., BOYD, C.B.: Lung cancer: A transmission and scanning electron microscope study. Cancer 29, 1530–1538 (1972).
NOWELL, J.A., GILLESPIE, J.R., TYLER, W.S.: Scanning electron microscopy of chronic pulmonary emphysema: A study of the equine model. Proceedings of the Fourth Annual Scanning Electron Microscope Symp., pp. 297–304. Chicago, Ill. 1971.
NOWELL, J.A., TYLER, W.S.: Scanning electron microscopy of the surface morphology of mammalian lungs. Amer. Rev. Resp. Dis. 103, 313–328 (1971).
PORT, C.D., HENRY, M.C., KAUFMAN, D.G., HARRIS, C.C., KETELS, K.V.: Acute changes in the surface morphology of hamster tracheobronchial epithelium following benzo(a)pyrene and ferric oxide administration. Cancer Res. 33, 2498–2506 (1973).
ROTH, J., MEYER, H.W.: Electron microscope studies in mammalian lungs by freeze-etching. Exp. Path. 7, 71–83 (1972).
TYLER, W.S., DELORIMIER, A.A., MANUS, A.G., NOWELL, J.A.: Surface morphology of hypoplastic and normal lungs from newborn lambs. Proceedings of Fourth Annual Scanning Electron Microscope Symp., pp. 305–312. Chicago, Ill. 1971.
WANG, N., THURLBECK, W.M.: Scanning electron microscopy of the lung. Human Path. 1, 227–231 (1970).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1974 Springer-Verlag Berlin · Heidelberg
About this chapter
Cite this chapter
Port, C.D., Baxter, D.W., Harris, C.C. (1974). Surface Morphology of Tracheal Epithelium in Vitamin A Deficiency and Reversal. In: Karbe, E., Park, J.F. (eds) Experimental Lung Cancer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-61939-7_24
Download citation
DOI: https://doi.org/10.1007/978-3-642-61939-7_24
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-61941-0
Online ISBN: 978-3-642-61939-7
eBook Packages: Springer Book Archive