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Phage Display of Combinatorial Peptide and Protein Libraries and Their Applications in Biology and Chemistry

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Combinatorial Chemistry in Biology

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 243))

Abstract

The enormous potential of combinatorial approaches for studying problems in biology and chemistry has been clearly demonstrated over the last several years. In particular, phage display has become one of the major techniques for the use of combinatorial peptide and protein libraries. The reasons for the success of phage display are, at least, threefold: (1) phage display of combinatorial libraries creates a direct link between phenotype (the selectable properties of interest from the displayed library) and genotype (their sequences); (2) it offers the possibility to select and amplify single clones out of large libraries; (3) it allows in vitro as well as in vivo selections in order to evolve peptides and proteins with novel activities. Reported applications include selections of peptides as lead compounds in pharmaceutical research, redesign of protein structures and protein—protein interactions, screening of cDNA libraries and enzyme design. This diversity in applications has been made possible by the development of a variety of display formats and selection schemes and an increasing understanding of the biology of filamentous bacteriophages.

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Authors and Affiliations

  1. Lehrstuhl für Organische Chemie I, Bioorganische Chemie, Ruhr-Universität Bochum, D-44780, Bochum, Germany

    K. Johnsson

  2. XERION PHARMACEUTICALS GmbH, Fraunhoferstr. 9, D-82152, Martinsried, Germany

    L. Ge

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  1. Kekule-Institut für Organische Chemie und Biochemie, Gerhard-Domagk-Str. 1, D-53121, Bonn, Germany

    Michael Famulok

  2. Institut für Biochemie — Genzentrum, Ludwig Maximilians Universität München, Feodor-Lynnen-Str. 25, D-81377, München, Germany

    Ernst-L. Winnacker

  3. The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Chi-Huey Wong

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Johnsson, K., Ge, L. (1999). Phage Display of Combinatorial Peptide and Protein Libraries and Their Applications in Biology and Chemistry. In: Famulok, M., Winnacker, EL., Wong, CH. (eds) Combinatorial Chemistry in Biology. Current Topics in Microbiology and Immunology, vol 243. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60142-2_5

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  • DOI: https://doi.org/10.1007/978-3-642-60142-2_5

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