Abstract
Due to the reducing conditions in common E.coli based cell-free protein Expression systems the formation of disulfide bonds is not possible. By changing the redox environment and by the addition of disulfide bond-forming catalysts it could be demonstrated that significant percentages of active disulfide-bonded proteins can be produced.
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A.R. Frand, I.W. Cuozzo, C.A. Kaiser (2000) Trends in Cell Biology, Vol. 10, p203
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© 2002 Springer-Verlag Berlin Heidelbreg
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Fernholz, E., Zaiss, K., Besir, H., Mutter, W. (2002). The Expression of Disulfide Bonded Proteins in Cell-Free Protein Expression. In: Spirin, A.S. (eds) Cell-Free Translation Systems. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59379-6_16
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DOI: https://doi.org/10.1007/978-3-642-59379-6_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-63956-2
Online ISBN: 978-3-642-59379-6
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