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Detection of Proteins on Two-Dimensional Electrophoresis Gels

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Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods

Part of the book series: Principles and Practice ((PRINCIPLES))

Abstract

In 2-D electrophoresis, the second dimension is most commonly the SDS separation. Consequently, detection on 2-D gels uses the methods developed for SDS gels. However, some issues which are often not so important in 1-D electrophoresis must be carefully taken care of in proteome-based approaches. Apart from the detection threshold, which is always a very important parameter, these issues include the dynamic range of detection, the linearity, the reproducibility between different staining experiments and the inter-protein variability. In addition, the interfacing of the staining methods with subsequent protein analysis methods (microsequencing, amino acid analysis, mass spectrometry) is more and more often an important parameter.

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Authors and Affiliations

  1. CEA-Laboratoire de Bioénergétique Cellulaire et Pathologique EA 2019, DBMS/BECP, CEA-Grenoble, 17 rue des Martyrs, F-38054, Grenoble Cedex 9, France

    T. Rabilloud & S. Charmont

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  1. T. Rabilloud
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  2. S. Charmont
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  1. CEA-Laboratoire de Bioénergétique Cellulaire et Pathologique EA 2019, DBMS/BECP, CEA-Grenoble, 17 rue des Martyrs, 38054, Grenoble Cedex 9, France

    Thierry Rabilloud

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© 2000 Springer-Verlag Berlin Heidelberg

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Rabilloud, T., Charmont, S. (2000). Detection of Proteins on Two-Dimensional Electrophoresis Gels. In: Rabilloud, T. (eds) Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57105-3_5

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  • DOI: https://doi.org/10.1007/978-3-642-57105-3_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-65792-7

  • Online ISBN: 978-3-642-57105-3

  • eBook Packages: Springer Book Archive

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