Abstract
Sequencing peptides was an enormously cumbersome process until the development of tandem mass spectrometry (MS/MS). The main barrier to broad use of this technique has been the difficulty of spectrum interpretation. The SEQUEST software package attempts to ease this process by automatically matching tandem mass spectra to database sequences (Eng et al. 1994). Candidate sequences are culled from the database by several simple filters. Virtual spectra are constructed for these sequences, and these are compared to the observed spectrum. Sequences yielding spectra most similar to the observed one are reported to the user.
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© 2001 Springer-Verlag Berlin Heidelberg
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Tabb, D.L., Eng, J.K., Yates, J.R. (2001). Protein Identification by SEQUEST. In: Proteome Research: Mass Spectrometry. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56895-4_7
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DOI: https://doi.org/10.1007/978-3-642-56895-4_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-67256-2
Online ISBN: 978-3-642-56895-4
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