Abstract
This chapter is a primer for those investigators working with lichens for whom molecular techniques are a relatively new experience, or who are having problems obtaining consistent results due to difficulties in deriving nucleic acids of sufficient purity. We will deal with some of the particular problems of nucleic acid extraction from lichens, focusing on genomic DNA isolation protocols, although we will also make some suggestions about RNA isolation. Some of the published protocols will be considered and a CTAB (Cetyl-trimethyl ammonium bromide) based protocol for lichen DNA isolation will be explained in detail.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Armaleo D, Clerc P (1991) Lichen chimeras: DNA analysis suggests that one fungus forms two morphotypes. Exp Mycol 15:1–10
Armaleo D, Clerc P (1995) A rapid and inexpensive method for the purification of DNA from lichens and their symbionts. Lichenologist 27:207–213
Bruns TD, Fogel R, Taylor JW (1990) Amplification and sequencing of DNA from fungal herbarium specimens. Mycologia 82:175–184
Crespo A, Bridge PD, Hawksworth DL (1997) Amplification of fungal rDNA-ITS regions from non-fertile specimens of the lichen-forming genus Parmelia. Lichenologist 29:275–282
Cubero OF, Crespo A, Fatehi J, Bridge, PF (1999) DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized and other fungi. Pl Syst Evol 216:243–249
Doyle JJ, Dickson EE (1987) Preservation of plant samples for DNA restriction endonuclease analysis. Taxon 36:715–722
Grube M, DePriest PT, Gargas A, Hafellner J (1995) DNA isolation from lichen ascomata. Mycol Res 99:1321–1324
Höss M, Jaruga P, Zastawny TH, Dizdaroglu M, Pääbo S (1996) DNA damage and DNA sequence retrieval from ancient tissues. Nuc Acids Res 24:1304–1307
Jones AS (1963) Use of alkyltrimethylammonium bromides for the isolation of ribo-and the deoxyribo-nucleic acids. Nature 199:280–282
Lee SB, Taylor JW (1990) Isolation of DNA from fungal mycelia and single spores. In: Innis MA, Gelfand DH, Sninsky JJ and White TJ (eds) PCR Protocols. Academic Press, San Diego. 282–287
Pich U, Schubert I (1993) Midiprep method for isolation of DNA from plants with a high content of polyphenolics. Nuc Acids Res 21:14
Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. Plant Molecular Biology Manual A6:1–10
Samsbrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Second Edition. Cold Spring Harbor Laboratory Press. New York
Savolainen V, Cuènoud P, Spichiger R, Martinez MDP, Crèvecoeur M, Manen J (1995) The use of herbarium specimens in DNA phylogenetics; evaluation and improvement. Pl Syst Evol 190:87–98
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Cubero, O.F., Crespo, A. (2002). Isolation of Nucleic Acids From Lichens. In: Kranner, I.C., Beckett, R.P., Varma, A.K. (eds) Protocols in Lichenology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56359-1_23
Download citation
DOI: https://doi.org/10.1007/978-3-642-56359-1_23
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-41139-0
Online ISBN: 978-3-642-56359-1
eBook Packages: Springer Book Archive